公开(公告)号：KR1020110121492A

公开(公告)日：2011-11-07

申请号：KR1020100041102

申请日：2010-04-30

Applicant: 서울대학교산학협력단

Inventor： 김윤영 ,  구승엽 ,  오선경 ,  문신용 ,  최영민

IPC: A01N1/02 ,  C12N5/0735 ,  C12N5/07

CPC classification number: A01N1/0221 ,  C12N5/0657

Abstract: PURPOSE: A composition containing ROCK(Rho-associated kinase) inhibitors for cryopreservation of cardiac myocytes from human embryonic stem cells is provided to develop a cell therapeutic agent. CONSTITUTION: A composition for cryopreservation of cardiac myocytes differentiated from human embryonic stem cells contains ROCK(Rho-associated kinase) inhibitor. The human stem cells are human embryonic stem cells, human embryonic stem cell-derived embryoid body, or human pluripotent stem cells. The ROCK inhibitor is Y-27632, HA-1077, Y-39983, or Wf-536. A method for cryopreservation of differentiated cardiac myocytes comprise: a step of treating the composition to the differentiated cardiac myocytes for 0.25-5 hours; and a step of freezing.

Abstract translation: 目的：提供一种含有ROCK（Rho相关激酶）抑制剂的组合物，用于从人胚胎干细胞中冷冻保存心肌细胞，以开发细胞治疗剂。 构成：用于从人胚胎干细胞分化的心肌细胞的低温保存组合物含有ROCK（Rho-相关激酶）抑制剂。 人类干细胞是人类胚胎干细胞，人类胚胎干细胞衍生的胚状体，或人类多能干细胞。 ROCK抑制剂是Y-27632，HA-1077，Y-39983或Wf-536。 用于分化心肌细胞的低温保存方法包括：将组合物处理至分化心肌细胞0.25-5小时的步骤; 并冻结一步。

公开(公告)号：KR101213279B1

公开(公告)日：2012-12-18

申请号：KR1020100041102

申请日：2010-04-30

Applicant: 서울대학교산학협력단

Inventor： 김윤영 ,  구승엽 ,  오선경 ,  문신용 ,  최영민

IPC: A01N1/02 ,  C12N5/0735 ,  C12N5/07

Abstract: 본발명은인간배아줄기세포로부터분화유도된심근세포동결보존용조성물에관한것으로, 구체적으로 ROCK(Rho-associated kinase 억제제) 억제제를포함하는, 인간배아줄기세포로부터분화유도된심근세포의동결보존용조성물, 인간배아줄기세포로부터유도된심근세포에상기조성물을처리하는단계를포함하는, 분화유도된심근세포를동결보존시키는방법에관한것이다.

公开(公告)号：KR1020110088260A

公开(公告)日：2011-08-03

申请号：KR1020100008085

申请日：2010-01-28

Applicant: 서울대학교산학협력단

Inventor： 김윤영 ,  구승엽 ,  오선경 ,  문신용 ,  최영민

IPC: C12N5/077 ,  C12N5/0735 ,  C12N5/02

Abstract: PURPOSE: A method for inducing differentiation of human embryonic stem cells to cardiac myocytes is provided. CONSTITUTION: A method for inducing differentiation of human embryonic stem cells to cardiac myocytes comprises: a step of culturing the embryonic stem cells in a medium containing activin A for one day after differentiation; and a step of culturing the embryonic stem cells in a serum-free medium containing BMP2 for 1-5 days. The culture is performed by direct differentiation without formation of embryo bud. The concentrate of activin A is 50-100 ng/ml.

Abstract translation: 目的：提供一种诱导人胚胎干细胞分化为心肌细胞的方法。 构成：诱导人胚胎干细胞向心肌细胞分化的方法包括：在分化后一天培养含有激活素A的培养基中的胚胎干细胞的步骤; 以及在含有BMP2的无血清培养基中培养胚胎干细胞1-5天的步骤。 培养通过直接分化而不形成胚芽进行。 活化素A的浓缩物为50-100ng / ml。

公开(公告)号：KR101044785B1

公开(公告)日：2011-06-27

申请号：KR1020100108975

申请日：2010-11-04

Applicant: 서울대학교산학협력단

Inventor： 최영민 ,  문신용 ,  오선경

IPC: C12N5/0735 ,  C12N5/02

Abstract: PURPOSE: A method for preparing human embryonic stem cells without components derived from animal is provided to differentiate the stem cells to various organs. CONSTITUTION: A method for preparing human embryonic stem cells in a culture system without components derived from animal comprises: a step of culturing human foreskin cells(ATCC, CRL-2429, USA) into a feeder layer using humanized CELLstart; a step of performing co-culturing an embryo of human 4-cell stage with the feeder layer; a step of removing zona pellucida using Tyrode's solution; a step of removing trophoblase by mechanical dissection to isolate an inner cell mass(ICM); a step of performing co-culture of the ICM on the feeder layer in a medium containing 15% knockout SR xeno-feee, 8ng/ml of bFGF; and a step of isolating ICM and performing co-culture of the ICM dome with the feeder layer.

Abstract translation: 目的：提供不含动物成分的人胚胎干细胞的方法，以将干细胞分化为各种器官。 构成：在没有源自动物的组分的培养系统中制备人胚胎干细胞的方法包括：使用人源化CELLstart将人包皮细胞（ATCC，CRL-2429，USA）培养到饲养层中的步骤; 用饲养层共同培养人类4细胞阶段的胚胎的步骤; 使用Tyrode溶液去除透明带的步骤; 通过机械解剖去除滋养层以分离内细胞团（ICM）的步骤; 在含有15％敲除SR xeno-feee，8ng / ml bFGF的培养基中，在饲养层上进行ICM的共培养的步骤; 以及分离ICM并执行ICM圆顶与馈线层的共同培养的步骤。