Abstract:
PURPOSE: A novel porcine transmissible gastroenteritis virus(TGEV) is provided to use in TGEV research as a genetic marker and to use as an active ingredient of vaccine composition. CONSTITUTION: A porcine transmissible gastroenteritis virus(TGEV) contains a spike comprising an amino acid of sequence number 1. The TGEV contains a nucleotide sequence encoding an amino acid sequence. The TGEV has deposit number KCTC 11494BP. The TGEV is detected by determining nucleotide variation at the site 655, 1122-1123, 1126, 1145-1153, 2446, 3704 and 4231-4232 of nucleotide sequence number 2. A vaccine composition contains the TGEV as an active ingredient.
Abstract:
본 발명의 일 구체예는 바이러스 항원 H1N1(A/Swine/Korea/GC0503/2005) 또는 H3N2(A/Swine/Korea/GC0407/2005)를 이용한 인플루엔자 혈청 진단 방법 및 이를 위한 혈청 진단용 키트에 관한 것이다. 돼지 인플루엔자, ELISA
Abstract:
PURPOSE: A multiplex PCR primer for detecting PERV(Porcine endogenous retrovirus) infection is provided to efficiently detect porcine mitochondria DNA and envelope glycoprotein(env) gene. CONSTITUTION: A pair for diagnosing of determining PERV infection comprises: a primer pair having an oligonucleotide of sequence numbers 5 and 6, which specifically encodes envA; a primer pair having an oligonucleotide of sequence numbers 7 and 8, which specifically encodes envB; a primer pair with an oligonucleotide of sequence enumber 9 and 10, which specifically encodes envC; and a primer pair with an oligonucleotide of sequence numbers 11 and 12, which specifically encodes pol.
Abstract:
PURPOSE: A method for manufacturing an ELISA kit using heparin-treated PRRSV antigen is provided to minimize non-specific reaction and to significantly detect a target antibody of PRRSV. CONSTITUTION: A method for manufacturing ELISA kit for detecting PRRSV antibody comprises: a step of mixing PRRSV antigen and heparin; and a step of coating the surface of a dish using the mixture. The PRRSV antigen is CP07-401-9, LCS-EU, or mixture of CP07-401-9 and LCS-EU. A method for diagnosing PRRSV comprises: a step of reacting porcine serum with a dish coated with heparin-treated PRRSV antigen and a dish coated with a control group; a step of reacting with a second antibody; and a step of calculating PRRSV titer.
Abstract:
본 발명은 돼지의 내인성 레트로바이러스(Porcine endogenous retrovirus, PERV)의 감염 여부 검출용 다중 PCR 프라이머, 상기 프라이머를 포함하는 바이러스 감염 여부 진단용 키트 및 이를 이용한 진단 방법에 관한 것이다. 본 발명의 프라이머를 이용한 다중 PCR 결과, 돼지 유래 세포주 뿐만 아니라, 돼지에서 채취한 간 조직, 혈액 및 모근으로부터 분리한 DNA에서 PERV의 아형에 상관없이 역전사 효소 유전자( pol ), 외피 당단백질(envelope glycoprotein, env ) 유전자 및 돼지 미토콘드리아 DNA가 높은 효율로 탐지되었으므로, 본 발명의 프라이머는 돼지의 PERV 감염 여부를 진단하는데 유용하게 사용할 수 있다. 돼지 내인성 레트로바이러스(Porcine endogenous retrovirus, PERV), 다중 PCR(multiplex PCR), 인공장기, 장기 이식
Abstract:
PURPOSE: An influenza serodiagnosis assay using virus antigen H1N1 or H3N2 is provided to economically and easily diagnose porcine influenza serum. CONSTITUTION: A serodiagnosis assay of influenza comprises: a step of contacting animal serum sample with influenza virus antigen H1N1(A/Swine/Korea/GC0503/2005)(KCTC-11078BP) or H3N2(A/Swine/Korea/GC0407/2005)(KCTC-11079BP); and a step of detecting the presence of antibody bound to influenza virus antigen in the serum sample. The detection is performed by measuring OD(optical density) through ELISA. The animal is pig. A kit for serodiagnosis assay of swine influenza contains influenza antigen H1N1(A/Swine/Korea/GC0503/2005)(KCTC-11078BP) or H3N2(A/Swine/Korea/GC0407/2005)(KCTC-11079BP).
Abstract translation:目的:提供使用病毒抗原H1N1或H3N2的流行性血清学诊断法,以便经济和容易地诊断猪流感血清。 构成:流感病毒血清诊断法包括:将动物血清样品与流感病毒抗原H1N1(A / Swine / Korea / GC0503 / 2005)(KCTC-11078BP)或H3N2(A / Swine / Korea / GC0407 / 2005)接触的步骤, (KCTC-11079BP); 以及检测血清样品中与流感病毒抗原结合的抗体的存在的步骤。 通过ELISA测量OD(光密度)来进行检测。 动物是猪。 用于猪流感病毒血清诊断试剂盒含有流感抗原H1N1(A / Swine / Korea / GC0503 / 2005)(KCTC-11078BP)或H3N2(A / Swine / Korea / GC0407 / 2005)(KCTC-11079BP)。