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公开(公告)号：KR101538089B1
公开(公告)日：2015-07-22
申请号：KR1020097005443
申请日：2006-12-20
Applicant: 서울대학교산학협력단
CPC classification number: C12N5/0696 , C12N2501/235 , C12N2502/03 , C12N2502/04 , C12N2502/13 , C12N2502/1305 , C12N2502/1311 , C12N2502/1317 , C12N2502/1323 , C12N2502/1329 , C12N2502/1335 , C12N2502/1341 , C12N2502/1347 , C12N2502/1352 , C12N2502/1358 , C12N2502/1364 , C12N2502/137 , C12N2502/1376 , C12N2502/1382 , C12N2502/1388 , C12N2502/1394
Abstract: 본발명은다음단계를포함하는배아줄기세포(ESC)-유사세포를제조하는방법에관한것이다: (a) 포유동물조직또는체액으로부터성체줄기세포를포함하는제1세포군을수득하는단계; (b) 상기단계 (a)에서의포유동물조직과상이한포유동물조직으로부터상기제1세포군과상이한제2체세포군을수득하는단계; (c) 상기제1세포군또는상기제2체세포군으로부터콜로니를형성하기위하여충분한시간동안상기제1세포군및 상기제2체세포군을배지에서공동배양하는단계; 및 (d) ESC-유사세포를제조하기위하여충분한시간동안상기콜로니의세포를계대배양하는단계.
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公开(公告)号：KR1020090074018A
公开(公告)日：2009-07-03
申请号：KR1020097005443
申请日：2006-12-20
Applicant: 서울대학교산학협력단
CPC classification number: C12N5/0696 , C12N2501/235 , C12N2502/03 , C12N2502/04 , C12N2502/13 , C12N2502/1305 , C12N2502/1311 , C12N2502/1317 , C12N2502/1323 , C12N2502/1329 , C12N2502/1335 , C12N2502/1341 , C12N2502/1347 , C12N2502/1352 , C12N2502/1358 , C12N2502/1364 , C12N2502/137 , C12N2502/1376 , C12N2502/1382 , C12N2502/1388 , C12N2502/1394
Abstract: 본발명은다음단계를포함하는배아줄기세포(ESC)-유사세포를제조하는방법에관한것이다: (a) 포유동물조직또는체액으로부터성체줄기세포를포함하는제1세포군을수득하는단계; (b) 상기단계 (a)에서의포유동물조직과상이한포유동물조직으로부터상기제1세포군과상이한제2체세포군을수득하는단계; (c) 상기제1세포군또는상기제2체세포군으로부터콜로니를형성하기위하여충분한시간동안상기제1세포군및 상기제2체세포군을배지에서공동배양하는단계; 및 (d) ESC-유사세포를제조하기위하여충분한시간동안상기콜로니의세포를계대배양하는단계.
Abstract translation: 提供胚胎干细胞（ESC）样细胞以从哺乳动物细胞产生ESC样细胞，而不用生殖细胞的操纵。 一种用于产生胚胎干细胞（ESC）样细胞的方法包括：从哺乳动物组织或体液获得含有成体干细胞的第一细胞群的步骤; 从其他哺乳动物的组织获得第二体细胞群的步骤; 共培养第一细胞群和第二体细胞群以从细胞群形成菌落的步骤; 以及将菌落细胞分培养足够时间以产生ESC样细胞的步骤。 第一个细胞组是含有两种或多种细胞类型的异质群体。 体细胞群是含有一种体细胞类型的同源群体。
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