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公开(公告)号：WO2011052818A1
公开(公告)日：2011-05-05
申请号：PCT/KR2009/006267
申请日：2009-10-28
Applicant: 서울대학교산학협력단 , 김효수 , 강현재 , 이은주 , 박영배
IPC: C12N5/0775 , C12N5/02 , A61K35/48
CPC classification number: C12N5/0662 , A61K35/48 , C12N2500/38 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/155 , C12N2501/165 , C12N2501/39 , C12N2506/02
Abstract: 본 발명은 인간 만능줄기세포로부터 중배엽 줄기세포를 생산하는 방법에 관한 것으로, 인간 만능줄기세포에서 배아체를 형성하는 단계; 상기 배아체를 조직배양접시에 부착 후 자연적으로 중배엽 줄기세포로 분화를 유도하는 단계; 및 상기 중배엽 줄기세포의 동일성을 유지하면서 계속 증식 배양하는 단계를 포함한다. 본 발명은 그 유전적 배경의 상이함과 무관하게 모든 인간 만능줄기세포에 광범위하게 적용될 수 있는 규정화된 중배엽 줄기세포 분화유도방법을 제공한다. 궁극적으로, 본 발명은 인간 만능줄기세포를 사용하여 재생의학 및 세포치료분야에서 필요로 하는 중배엽 줄기세포를 지속적으로 대량 공급함으로써 세포치료제의 실용화를 가능하게 하고, 나아가 심혈관계질환, 신경계질환 등의 난치병 치료에 크게 기여할 수 있을 것으로 기대된다.
Abstract translation: 本发明涉及从人多能干细胞产生间充质干细胞的方法，包括以下步骤：从人多能干细胞形成胚体; 将胚胎体附着到培养皿中以诱导胚胎体自然分化成间充质干细胞; 并增殖间充质干细胞，同时仍维持间充质干细胞的特性。 本发明还涉及用于诱导间充质干细胞分化的标准化方法，其可以广泛地应用于所有人多能干细胞，而不管遗传背景的差异。 最终，本发明可以提供使用人多能干细胞的恒定和定量大量的间充质干细胞，其是再生医学和干细胞治疗所需的。 因此，可以实现细胞治疗剂的实际应用，并且期望由本发明高度受益于心血管疾病和神经障碍等困难病例的治疗。
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公开(公告)号：KR1020150137341A
公开(公告)日：2015-12-09
申请号：KR1020140065031
申请日：2014-05-29
Applicant: 서울대학교산학협력단 , 성균관대학교산학협력단
CPC classification number: C12M3/00 , C12N5/0068 , C12N5/0607 , C12N2533/74
Abstract: 본발명은, 다공성 3차원적층담체를이용하여중간엽줄기세포를연골세포특이적으로분화시키는방법, 이방법에의해분화된연골세포함유다공성 3차원적층담체, 및이 담체를포함하는관절염치료제에관한것이다. 본발명에의하면, 별도의연골세포분화유도배지를사용하지않고도간단하고도효율적인방법으로, 중간엽줄기세포를연골세포특이적으로분화시킬수 있는조직공학적방법을제공할수 있으며, 본발명의방법에의하여제조된중간엽줄기세포함유다공성 3차원적층담체는, 3차원구조가안정적이고, 공극간상호연결성이우수하여, 세포의생존과성장에필요한산소와양분의공급이원활히이루어져세포생존율이우수하기때문에, 유용한세포성장판및 관절염치료제로이용될수 있다.
Abstract translation: 本发明涉及一种使用多孔三维层状载体将间充质干细胞特异性分化为软骨细胞的方法，涉及含有通过该方法分化的软骨细胞的多孔三维层叠支架，以及包含该支持体的关节炎治疗剂。 根据本发明，提供了一种组织工程方法，其可以通过使用简单有效的方法将间充质干细胞特异性地分化成软骨细胞，而不使用设计用于诱导软骨细胞分化的单独培养基。 包含通过本发明的方法制备的间充质干细胞的多孔三维层压支架具有稳定的三维结构，并且在气隙之间具有优异的互连性，因此平稳地提供氧气和细胞存活和生长所需的营养物质。 因此，本发明的多孔三维层叠载体具有优异的细胞存活率，因此有用地用作关节炎和细胞生长板的治疗剂。
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公开(公告)号：KR101135636B1
公开(公告)日：2012-04-17
申请号：KR1020090102458
申请日：2009-10-27
Applicant: 서울대학교산학협력단
IPC: C12N5/074 , A61K35/545
CPC classification number: C12N5/0662 , A61K35/48 , C12N2500/38 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/155 , C12N2501/165 , C12N2501/39 , C12N2506/02
Abstract: Provided is a method for producing mesenchymal stem cells from human pluripotent stem cells, the method including: a) forming embryonic bodies from human pluripotent stem cells; b) attaching the embryonic bodies to a culture dish to induce natural differentiation of the embryonic bodies into mesenchymal stem cells; and c) performing continuous proliferative culturing of the mesenchymal stem cells while still maintaining the identity of the mesenchymal stem cells. Also, provided is a standardized method for inducing differentiation of mesenchymal stem cells, which can be broadly applied to all human pluripotent stem cells regardless of a difference in the genetic background thereof. Ultimately, the present invention can continuously mass-produces the mesenchymal stem cells necessary for regenerative medicine and cell therapy by using human pluripotent stem cells, thereby realizing practical uses of cell therapy products, and further the present invention is expected to highly contribute to treatments of incurable diseases, such as cardiovascular diseases and neurological disorders.
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公开(公告)号：KR1020150018204A
公开(公告)日：2015-02-23
申请号：KR1020130094833
申请日：2013-08-09
Applicant: 서울대학교산학협력단
IPC: C12N5/0775 , C12N5/0735 , C12N5/02 , C07K14/475 , A61K38/18
Abstract: The present invention relates to a method for preparing a stem cell aggregate having improved engraftment, and a composition for preventing and treating ischemic disease, comprising a stem cell aggregate prepared by the method. More specifically, it was established that the present invention can be effectively used to prevent or treat ischemic disease by verifying that, when stem cells are suspension-cultured to construct a stem cell aggregate within a null-additive basal medium not containing bovine serum, which is derived from an animal, a buffer solution having deteriorated safety, and antibiotic components capable of increasing resistance, the stem cell aggregate produces a large amount of vasculogenic factors capable of improving the neovascularization of ischemic tissues, and when the stem cell aggregate is transplanted in the ischemic tissue, the engraftment ratio significantly increases compared with single cell transplantation.
Abstract translation: 本发明涉及一种制备具有改善植入物的干细胞聚集体的方法，以及用于预防和治疗缺血性疾病的组合物，其包含通过该方法制备的干细胞聚集体。 更确切地说，确定本发明可以有效地用于预防或治疗缺血性疾病，通过验证当干细胞被悬浮培养以在不含牛血清的无效添加剂基础培养基内构建干细胞聚集体时，其中 衍生自动物，安全性降低的缓冲溶液和能够增加抗性的抗生素成分，干细胞聚集体产生能够改善缺血组织新生血管形成的大量血管生成因子，并且当将干细胞聚集体移植到 与单细胞移植相比，缺血组织，植入率显着增加。
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公开(公告)号：KR1020120025117A
公开(公告)日：2012-03-15
申请号：KR1020100087303
申请日：2010-09-07
Applicant: 서울대학교산학협력단
CPC classification number: A23L13/40 , A23L3/26 , A23V2002/00
Abstract: PURPOSE: A method of removing bad smell from ground beef cooked by irradiating with radiation is provided to use garlic, onion, or their mixture for preventing the oxidation of the beef. CONSTITUTION: A method of removing bad smell from ground beef cooked by irradiating with radiation comprises the following steps: adding more than one ingredient selected from garlic or onion to raw beef; and storing the mixture at 4 deg C for 8-15 hours before irradiating the mixture with radiation. The mixture contains 0.01-0.2wt% of garlic and 0.2-5wt% of onion. The intensity of the radiation for the irradiation process is 0.5-5kGy.
Abstract translation: 目的：采用大蒜，洋葱或其混合物来防止牛肉氧化，从而通过辐射照射而煮熟的牛肉中消除不良气味的方法。 构成：通过辐射辐射煮熟的牛肉去除不良气味的方法包括以下步骤：向生牛肉中加入多种选自大蒜或洋葱的成分; 并将混合物在4℃下储存8-15小时，然后用辐射照射混合物。 该混合物含有0.01-0.2重量％的大蒜和0.2-5重量％的洋葱。 照射过程的辐射强度为0.5-5kGy。
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Patent Agency Ranking
公开(公告)号：KR1020110045760A
公开(公告)日：2011-05-04
申请号：KR1020090102458
申请日：2009-10-27
Applicant: 서울대학교산학협력단
IPC: C12N5/074 , A61K35/545
CPC classification number: C12N5/0662 , A61K35/48 , C12N2500/38 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/155 , C12N2501/165 , C12N2501/39 , C12N2506/02 , C12N5/0696 , A61K35/545
Abstract: PURPOSE: A method for producing mesenchymal stem cells from human pluripotent stem cells is provided to continuously produce a large amount of mesenchymal stem cells. CONSTITUTION: A method for producing mesenchymal stem cells from human pluripotent stem cells comprises: a step of forming an embryoid body from the human pluripotent stem cells; a step of culturing the embryoid body to naturally induce differentiation to mesenchymal stem cells; a step of culturing and proliferating the mesenchymal stem cells. A cell therapeutic agent contains the mesenchymal stem cells. The cell therapeutic agent is used for forming adipocytes, osteocytes, chondrocytes, muscular cells, or nerve cells.
Abstract translation: 目的：提供从人多能干细胞产生间充质干细胞的方法，以连续产生大量的间充质干细胞。 构成：从人多能干细胞产生间充质干细胞的方法包括：从人多能干细胞形成胚状体的步骤; 培养胚状体以自然诱导分化成间充质干细胞的步骤; 培养和增殖间充质干细胞的一个步骤。 细胞治疗剂含有间充质干细胞。 细胞治疗剂用于形成脂肪细胞，骨细胞，软骨细胞，肌细胞或神经细胞。
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公开(公告)号：KR102037823B1
公开(公告)日：2019-10-29
申请号：KR1020130094832
申请日：2013-08-09
Applicant: 서울대학교산학협력단
IPC: C07K14/475 , C12P21/00 , A61K38/18
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公开(公告)号：KR1020150018203A
公开(公告)日：2015-02-23
申请号：KR1020130094832
申请日：2013-08-09
Applicant: 서울대학교산학협력단
IPC: C07K14/475 , C07K1/14 , C12N5/0775 , C12N5/0735
Abstract: The present invention relates to a method for mass-producing angiogenesis factors using stem cell aggregates and a composition for treating and preventing ischemic diseases including culture medium of the stem cell aggregates. More specifically, in the present invention, stem cell aggregates are made in a buffer solution, which has less stability and less fetal bovine serum derived from animals and a non-additive basal medium, which has no antibiotics to increase tolerance via a suspension culture, thereby mass-producing angiogenesis factors from the stem cell aggregate culture medium while the survival rate of the angiogenesis factor remarkably increases, when transplanted to ischemic tissues, compared with that of transplanted single cells, so the composition of the present invention can be used for efficiently treating or preventing ischemic diseases.
Abstract translation: 本发明涉及使用干细胞聚集体大规模生产血管生成因子的方法和用于治疗和预防包括干细胞聚集体的培养基的缺血性疾病的组合物。 更具体地说，在本发明中，干细胞聚集体制成缓冲溶液，该缓冲溶液具有较少的稳定性和较少的来自动物的胎牛血清和非添加剂的基础培养基，该培养基没有抗生素通过悬浮培养增加耐受性， 从而与移植的单细胞相比，干细胞聚集培养基的血管生成因子大量生成，同时血管生成因子的存活率显着增加，移植到缺血组织中，因此本发明的组合物可以有效地使用 治疗或预防缺血性疾病。
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公开(公告)号：KR101147674B1
公开(公告)日：2012-05-24
申请号：KR1020100087303
申请日：2010-09-07
Applicant: 서울대학교산학협력단
Abstract: PURPOSE: A method of removing bad smell from ground beef cooked by irradiating with radiation is provided to use garlic, onion, or their mixture for preventing the oxidation of the beef. CONSTITUTION: A method of removing bad smell from ground beef cooked by irradiating with radiation comprises the following steps: adding more than one ingredient selected from garlic or onion to raw beef; and storing the mixture at 4 deg C for 8-15 hours before irradiating the mixture with radiation. The mixture contains 0.01-0.2wt% of garlic and 0.2-5wt% of onion. The intensity of the radiation for the irradiation process is 0.5-5kGy.
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公开(公告)号：KR1020120025116A
公开(公告)日：2012-03-15
申请号：KR1020100087301
申请日：2010-09-07
Applicant: 서울대학교산학협력단
CPC classification number: A23L13/40 , A23L3/263 , A23L5/20 , A23L5/30 , A23V2002/00
Abstract: PURPOSE: A bad smell removal method of raw ground beef irradiated with radiation using garlic or onion is provided to effectively prevent the oxidation of the beef. CONSTITUTION: A bad smell removal method of raw ground beef comprises the following steps: inserting garlic, onion, or a mixture thereof to raw ground beef; and storing the mixture for 8-15 hours at 4 deg C before irradiating the mixture with radiation. The mixture contains less than 0.1wt% of garlic and more than 0.5wt% of onion.
Abstract translation: 目的：提供用大蒜或洋葱辐射的生碎牛肉的不良气味去除方法，以有效防止牛肉的氧化。 构成：原料牛肉的不良气味去除方法包括以下步骤：将大蒜，洋葱或其混合物加入生牛肉中; 并在4摄氏度下将混合物储存8-15小时，然后用辐射照射混合物。 混合物含有小于0.1wt％的大蒜和大于0.5wt％的洋葱。
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