히알루론산과 알지네이트를 포함하는 상호관입고분자망목 구조의 세포전달용 지지체의 제조방법
    1.
    发明公开
    히알루론산과 알지네이트를 포함하는 상호관입고분자망목 구조의 세포전달용 지지체의 제조방법 有权
    聚合物网络(IPN)SCAFFED的制备方法用于包含羟丙基钠和硫酸钠的细胞递送

    公开(公告)号:KR1020120086948A

    公开(公告)日:2012-08-06

    申请号:KR1020110008315

    申请日:2011-01-27

    Abstract: PURPOSE: A manufacturing method of interpenetrating polymer network scaffold for cell delivery which includes hyaluronic acid and alginate are provided to provide porous hyaluronic acid-alginate supporter which recycles chondrocyte and enoughly delivers nutrients. CONSTITUTION: A manufacturing method of interpenetrating polymer network scaffold for cell delivery which includes hyaluronic acid and alginate comprises the following steps: melting hyaluronic acid and alginate into a sodium hydroxide aqueous solution; adding an epoxy-based cross linking agent and homogenizing the hyaluronic acid-alginate solution; hydrogelizing the homogenized hyaluronic acid-alginate solutions; washing the hydrogel with a calcium chloride aqueous solution; obtaining porosity by expanding the washed hyaluronic acid-alginate in a calcium chloride aqueous solution; eliminating calcium chloride remaining in the hyaluronic acid - alginate supporter using distilled water; and obtaining a porous hyaluronic acid supporter by freeze-drying the hydrogel.

    Abstract translation: 目的:提供包含透明质酸和藻酸盐的细胞递送的互穿聚合物网络支架的制造方法,以提供多孔透明质酸藻酸盐载体,其回收软骨细胞并足够输送营养物质。 构成:包含透明质酸和藻酸盐的细胞递送的互穿聚合物网络支架的制造方法包括以下步骤:将透明质酸和藻酸盐溶解成氢氧化钠水溶液; 加入环氧基交联剂并均化透明质酸 - 藻酸盐溶液; 水化均匀的透明质酸 - 藻酸盐溶液; 用氯化钙水溶液洗涤水凝胶; 通过在氯化钙水溶液中膨胀洗涤的透明质酸藻酸盐来获得孔隙度; 使用蒸馏水去除透明质酸 - 藻酸盐载体中残留的氯化钙; 并通过冷冻干燥水凝胶得到多孔透明质酸载体。

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