公开(公告)号：KR101469849B1

公开(公告)日：2014-12-08

申请号：KR1020080027881

申请日：2008-03-26

Applicant: 서울대학교산학협력단

Inventor： 김빛내리 ,  허인하 ,  주철민

IPC: C12Q1/68

Abstract: 본원발명은마이크로RNA 활성측정방법에관한것으로, 더욱상세하게는본발명의방법은우리딘화를검출하여 miRNA 생성시우리딘화에영향을미치는피검화합물선별하는데유용하게이용될수 있다.

公开(公告)号：KR1020090103964A

公开(公告)日：2009-10-05

申请号：KR1020080027881

申请日：2008-03-26

Applicant: 서울대학교산학협력단

Inventor： 김빛내리 ,  허인하 ,  주철민

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12N15/63 ,  G01N33/5008

Abstract: PURPOSE: A method for measuring microRNA activity is provided to detect uridinylation and screen a test sample compound which affects uridinylation when miRNA is generated by detecting uridinylation. CONSTITUTION: A method for screening uridinylation controlling compound comprises: a step of transducing an expression vector which expresses Lin28 protein in a test cell line; a step of treating a test sample compound to the transformed test cell line; and a step of screening uridinylated miRNA by grinding the treated test cell line.

Abstract translation: 目的：提供一种测量微小RNA活性的方法，以检测尿苷酸化，并筛选出通过检测尿苷酸化产生miRNA产生影响尿苷酸化的测试样品化合物。 构成：筛选尿苷酸化控制化合物的方法包括：在测试细胞系中转导表达Lin28蛋白的表达载体的步骤; 将测试样品化合物处理转化的测试细胞系的步骤; 以及通过研磨处理的测试细胞系筛选尿苷酸化的miRNA的步骤。