公开(公告)号：KR1020100062336A

公开(公告)日：2010-06-10

申请号：KR1020080120932

申请日：2008-12-02

Applicant: 서울대학교산학협력단

Inventor： 홍석철 ,  이남경 ,  이경진 ,  박진성 ,  현주용

IPC: C12Q1/68 ,  G01N33/48

CPC classification number: G06F19/16 ,  C12Q1/68

Abstract: PURPOSE: A method for measuring elasticity of DNA targeting drug-bound DNA and a method for measuring binding degree of DNA and DNA targeting drug are provided to find physical property of DNA. CONSTITUTION: A method for measuring elasticity of DNA targeting drug-bound DNA is performed by measuring tension and length to a binding DNA. A method for measuring relation of tension and length is defined by WLC having changing continuous length by formula 3 in force-length curve of WLC model of formula 1 in case of lack of interelation between kinks generated by binding. The binding with DNA targeting drug is performed in a proper tension and salt concentration.

Abstract translation: 目的：提供一种测定靶向药物结合DNA的DNA弹性的方法以及测定DNA和DNA靶向药物结合程度的方法，以找出DNA的物理性质。 构成：通过测量结合DNA的张力和长度来测量靶向药物结合的DNA的DNA的弹性的方法。 用于测量张力和长度关系的方法由WLC定义，WLC在结合产生的扭结之间缺乏相互作用的情况下，通过公式3的WLC模型的力 - 长度曲线中具有连续长度改变的连续长度。 与DNA靶向药物的结合以适当的张力和盐浓度进行。