公开(公告)号：KR1020140048002A

公开(公告)日：2014-04-23

申请号：KR1020120114456

申请日：2012-10-15

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  강솔 ,  박정안 ,  길의준

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/04 ,  C12Q2527/101

Abstract: The present invention relates to a primer set for an isothermal amplification reaction to detect Chrysanthemum stunt viroid (CSVd), a composition comprising the primer set, and a method for detecting CSVd using the composition. When the primer set for an isothermal amplification reaction to detect CSVd according to the present invention is used, the CSVd can be effectively detected without technical equipment on the spot for a short period of time. In addition, viroids can be detected even with a small amount of sample, and the diagnostic results can be promptly verified by the naked eye under the natural light by using a high-concentration of SYBR Green I. Therefore, Chrysanthemum infectious viroids that seriously damage Chrysanthemum farmhouses can be detected early, promptly, and accurately, thereby significantly reducing a huge economic loss due to the Chrysanthemum infectious viroids.

Abstract translation: 本发明涉及用于检测菊花特发性类病毒（CSVd）的等温扩增反应的引物组，包含引物组的组合物，以及使用该组合物检测CSVd的方法。 当使用根据本发明的用于检测CSVd的等温扩增反应的引物时，可以在没有技术设备的情况下，在现场短时间内有效地检测CSVd。 此外，即使使用少量的样品也能够检测到病毒，并且通过使用高浓度的SYBR Green I，可以在自然光下通过肉眼迅速验证诊断结果。因此，严重损害菊花感染性病毒 可以及早，准确地检测到菊花农舍，从而大大减少了菊花感染性病毒的巨大经济损失。