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    使用PKD2基因的CYST表达转基因动物的生产方法

    公开(公告)号:KR1020070083375A

    公开(公告)日:2007-08-24

    申请号:KR1020060016662

    申请日:2006-02-21

    Abstract: A production method of a cyst expressed transgenic animal using PKD2 gene is provided to obtain a production method of an animal that expresses cyst only by the over expression of the PKD2 gene. A production method of a cyst expressed transgenic animal using PKD2 gene includes: a first step of manufacturing PKD2 protein expressing vector; a second step of producing a fertilized egg by inserting the expressed vector into the nucleus of the fertilized egg; and a third step of producing transgenic animal by planting the produced fertilized egg into the womb of a surrogate mother. A plasmid is cut by using restriction enzyme Xbal and Xhol(Promega) and introducing PKD2 gene to prepare the expression vector.

    Abstract translation: 提供使用PKD2基因的囊肿表达的转基因动物的制备方法,以获得仅通过PKD2基因的过表达来表达囊肿的动物的制备方法。 使用PKD2基因的囊肿表达的转基因动物的制备方法包括:制备PKD2蛋白质表达载体的第一步; 通过将表达的载体插入受精卵的细胞核中来产生受精卵的第二步骤; 以及通过将产生的受精卵种植到替代母亲的子宫中来生产转基因动物的第三步骤。 通过使用限制性内切酶XbaI和XhoI(Promega)切割质粒并引入PKD2基因以制备表达载体。

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