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1.发明授权
公开(公告)号：KR101344160B1
公开(公告)日：2013-12-23
申请号：KR1020110118418
申请日：2011-11-14
Applicant: 숭실대학교산학협력단
Abstract: 본발명은ⅰ)단백질코팅된금속나노입자를제조하는단계; ⅱ)상기코팅된단백질의질량을수정미세저울(QCM)을이용하여측정하는단계; ⅲ)상기나노입자를세포내로흡수시키고이를암시야현미경기술(DFM)을이용하여탐지하는단계; 및ⅳ)z-depth 의존공초점표면증강라만산란(SERS)을이용하여나노입자를탐지하는단계;를포함하는단백질코팅된금속나노입자를이용하여단세포내 흡수된단백질의질량과위치를측정하는방법및 장치에관한것이다.본발명에따르면단백질코팅된금속나노입자를이용하여세포내로흡수되는단백질의질량을측정할수 있고, 단백질의세포내 진입과정을트래킹(tracking) 및이미징(imaging)할수 있어나노입자를이용한약물전달시 이를미리제어하여원하는양과위치에약물을전달할수 있으며, 단백질과나노입자간 상호작용을이해하는데도움을줄 수있다.
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2.发明公开
公开(公告)号：KR1020130052995A
公开(公告)日：2013-05-23
申请号：KR1020110118418
申请日：2011-11-14
Applicant: 숭실대학교산학협력단
Abstract: PURPOSE: A method and an apparatus for measuring mass and location of proteins in a single cell using a protein-coated metal nanoparticle are provided to deliver a drug to a desired site. CONSTITUTION: A method for measuring mass and location of proteins in a single cell using a protein-coated metal nanoparticle comprises: a step of preparing the protein-coated metal nanoparticle; a step of measuring the mass of the protein by QCM(quartz crystal microbalance); a step of absorbing the protein-coated metal nanoparticle into a cell and detecting the nanoparticle using DFM(dark field microscopy); and a step of detecting the protein-coated metal nanoparticle using SERS(surface-enhanced raman spectroscopy).
Abstract translation: 目的：提供一种用于使用蛋白质包被的金属纳米颗粒测量单个细胞中的蛋白质的质量和位置的方法和装置，以将药物递送至期望的部位。 构成：使用蛋白质包被的金属纳米颗粒测量单个细胞中蛋白质的质量和位置的方法包括：制备蛋白质包被的金属纳米颗粒的步骤; 通过QCM（石英晶体微量天平）测量蛋白质的质量的步骤; 将蛋白质包被的金属纳米颗粒吸收到细胞中并使用DFM（暗视野显微镜）检测纳米颗粒的步骤; 以及使用SERS（表面增强拉曼光谱）检测蛋白质包被的金属纳米粒子的步骤。
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