公开(公告)号：KR101455360B1

公开(公告)日：2014-10-28

申请号：KR1020110112974

申请日：2011-11-01

Applicant: 아주대학교산학협력단

Inventor： 이평천 ,  김종래

IPC: C12N1/21 ,  C12N15/70 ,  C12N15/52 ,  C12P23/00

Abstract: 본 발명은 대사회로를 재구축하여 수크로오스를 이용할 수 있도록 형질전환된 대장균에 관한 것이며, 이를 이용하여 수크로오스로부터 2차 대사산물을 생산할 수 있도록 하는 방법에 관한 것이다.

公开(公告)号：KR1020130048058A

公开(公告)日：2013-05-09

申请号：KR1020110112974

申请日：2011-11-01

Applicant: 아주대학교산학협력단

Inventor： 이평천 ,  김종래

IPC: C12N1/21 ,  C12N15/70 ,  C12N15/52 ,  C12P23/00

Abstract: PURPOSE: Recombinant E. coli with reconstructed sucrose metabolic pathways is provided to be used in a microorganism-based industry by transforming E.coli which produces a secondary metabolite using only sucrose as a carbides source. CONSTITUTION: Sucrose-using E.coli is a reconstructed PTS(phosphoenolpyruvate (PEP)-dependent phosphotransferase transport system) system or non-PTS system. The PTS system is prepared by transduction of sucrose phosphotransferase, sucrose hydrolase, and fructokinase. The non-PTS system is prepared by transduction of sucrose permease, sucrose hydrolase or phophorylase, and fructokinase. The PTS-syste-introduced E.coli is prepared by transformation with pAC-AAK plasmid which expresses scrA, sacA, and sack genes. The gene is derived from Staphylococcus xylosus and Lactobacillus plantarum.

Abstract translation: 目的：重组蔗糖代谢途径的重组大肠杆菌提供用于基于微生物的工业，通过转化大肠杆菌，其仅使用蔗糖作为碳化物源产生次级代谢物。 构成：使用蔗糖的大肠杆菌是重建的PTS（磷酸烯醇丙酮酸（PEP）依赖性磷酸转移酶转运系统）系统或非PTS系统。 PTS系统是通过转导蔗糖磷酸转移酶，蔗糖水解酶和果糖激酶来制备的。 非PTS系统通过蔗糖通透酶，蔗糖水解酶或磷酸化酶和果糖激酶的转导来制备。 通过用表达scrA，sacA和sack基因的pAC-AAK质粒转化来制备PTS-系统引入的大肠杆菌。 该基因来源于木糖葡萄球菌和植物乳杆菌。