公开(公告)号：KR1020030033719A

公开(公告)日：2003-05-01

申请号：KR1020010065808

申请日：2001-10-24

Applicant: 재단법인서울대학교산학협력재단 ,  주식회사 바이오앤진 ,  주식회사 보락

Inventor： 서진호 ,  고영호 ,  김명동 ,  정수련 ,  김상용

IPC: C12Q1/68

Abstract: PURPOSE: Provided are a method of quantitatively and qualitatively analyzing lactic acid bacteria and a PCR primer which is capable of quantitatively and qualitatively analyzing lactic acid bacteria present in fermented foods. The quantitative and qualitative analysis can be carried out by performing QC-PCR with a PlaF/PlaR primer and an MesF/MesR primer to determine the fermentation degree and aging degree of fermented foods, and the storage state and quality thereof. CONSTITUTION: A method for quantitatively analyzing lactic acid bacteria comprises the step of: adding a target DNA to a sample and mixing a competitor Cmes or Cpla and performing PCR with a specific primer set of Lactobacillus plantarum or a specific primer set of Leuconostoc mesenteroides; measuring PCR amplification of the competitor and target DNA; and calculating the concentration of the target DNA concentration from the PCR amplification rate and the concentration of the competitor.

Abstract translation: 目的：提供定量分析乳酸菌的方法和能够定量分析发酵食品中存在的乳酸菌的PCR引物。 可以通过使用PlaF / PlaR引物和MesF / MesR引物进行QC-PCR来确定发酵食品的发酵程度和老化程度及其储存状态和质量来进行定量和定性分析。 构成：用于定量分析乳酸菌的方法包括以下步骤：向样品中加入靶DNA并混合竞争剂Cmes或Cpla，并用特异性引物组植物乳杆菌或特定引物组的肠导线突触菌素进行PCR; 测量竞争者和靶DNA的PCR扩增; 并根据PCR扩增率和竞争者的浓度计算目标DNA浓度的浓度。

公开(公告)号：KR100781386B1

公开(公告)日：2007-11-30

申请号：KR1020050097034

申请日：2005-10-14

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김명동 ,  이태희 ,  오용주 ,  서진호

IPC: C12N15/12

Abstract: 본 발명은 히루딘 단백질을 코딩하는 유전자가 도입된 재조합 사카로마이세스 세레비지애(
Saccharomyces cerevisiae )에 프로테인다이설파이드아이소머라아제1를 코딩하는 유전자 및 이알오1을 코딩하는 유전자를 동시 도입시켜 히루딘 단백질을 생산하는 방법에 관한 것으로, 히루딘 단백질을 유가식 배양에 의하여 최종농도 1.1 g/L를 얻을 수 있다.
히루딘, 효모, 프로테인다이설파이드아이소머라아제1, 이알오1

公开(公告)号：KR100658534B1

公开(公告)日：2006-12-19

申请号：KR1020050035445

申请日：2005-04-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이태희 ,  김명동 ,  오용주 ,  서진호

IPC: C12N15/09

Abstract: 본 발명은 제한효소 절단에 의한 클로닝 방법으로 표적 유전자를 파쇄하는 방법에 관한 것으로 유전자 파쇄에 있어 공지의 방법에 비해 향상된 정확도와 편리성, 증대된 형질전환 효율을 제공하는 뛰어난 효과가 있다.
유전자 파쇄, 형질전환, 사카로마이스세스 세레비지에

公开(公告)号：KR1020090057760A

公开(公告)日：2009-06-08

申请号：KR1020070124481

申请日：2007-12-03

Applicant: 재단법인서울대학교산학협력재단 ,  강원대학교산학협력단

Inventor： 서진호 ,  안지현 ,  김명동 ,  조제원

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q2545/107

Abstract: A primer kit for detecting Lactobacillus brevis is provided to accurately monitor the Lactobacillus brevis from a fermentation material such as Kimchi. A primer kit for detecting a Lactobacillus brevis comprises: a primer set which contains a forward primer of the sequence number 5 and reverse primer of the sequence number 6 and amplifies a part of sequence in 16S rRNA gene of Lactobacillus brevis; a primer set which contains a forward primer of the sequence number 3 and reverse primer of the sequence number 4 and amplifies a part of sequence in dextransucrase gene; and a primer set which contains a forward primer of the sequence number 1 and reverse primer of the sequence number 2.

Abstract translation: 提供了一种用于检测短乳杆菌的引物试剂盒，用于从泡菜等发酵材料中准确地监测短乳杆菌（Lactobacillus brevis）。 用于检测短乳杆菌的引物试剂盒包括：引物组，其含有序列号5的正向引物和序列号6的反向引物，并扩增短乳杆菌16S rRNA基因中的一部分序列; 包含序列号3的正向引物和序列号4的反向引物的引物组，并扩增葡聚糖蔗糖酶基因中的一部分序列; 以及包含序列号1的正向引物和序列号2的反向引物的引物组。

公开(公告)号：KR1020070041166A

公开(公告)日：2007-04-18

申请号：KR1020050097034

申请日：2005-10-14

Applicant: 재단법인서울대학교산학협력재단

Inventor： 김명동 ,  이태희 ,  오용주 ,  서진호

IPC: C12N15/12

Abstract: 본 발명은 히루딘 단백질을 코딩하는 유전자가 도입된 재조합 사카로마이세스 세레비지애(
Saccharomyces cerevisiae )에 프로테인다이설파이드아이소머라아제1를 코딩하는 유전자 및 이알오1을 코딩하는 유전자를 동시 도입시켜 히루딘 단백질을 생산하는 방법에 관한 것으로, 히루딘 단백질을 유가식 배양에 의하여 최종농도 1.1 g/L를 얻을 수 있다.
히루딘, 효모, 프로테인다이설파이드아이소머라아제1, 이알오1

公开(公告)号：KR1020060112809A

公开(公告)日：2006-11-02

申请号：KR1020050035445

申请日：2005-04-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이태희 ,  김명동 ,  오용주 ,  서진호

IPC: C12N15/09

Abstract: A method for disrupting a target gene based on cloning through restriction enzyme cutting is provided to show improved accuracy, convenience and increased transformation efficiency compared to conventional methods, thereby being useful for genetic engineering and bio-industries. In the method for disrupting a target gene by inserting a nucleotide sequence fragment(c) which includes a nucleotide sequence fragment(a) located at the upper portion of 5' terminal at an open leading frame starting point of the target frame, and a nucleotide sequence fragment(b) from an open leading frame starting point of a selective marker to a certain point of the inside of the open leading frame starting point of the selective marker in sequence from the 5' terminal side; and a nucleotide fragment(f) which includes a nucleotide sequence fragment(d) from the starting point formed to have an overlapped portion with the nucleotide sequence fragment(b) to an open leading frame ending point of the selective marker and a nucleotide sequence fragment(e) located at the lower portion of 3' terminal at the open leading frame ending point of the target gene in sequence from the 5' terminal side into a host, the nucleotide sequence fragment(c) is obtained from the nucleotide sequence fragment(a) inserted via a restriction enzyme site and a vector(A) including all gene sequence of the selective marker and the nucleotide sequence fragment(f) is obtained from the all gene sequence of the selective marker and a vector(B) including the nucleotide sequence fragment(e) inserted via the restriction enzyme site.

Abstract translation: 提供了一种基于通过限制酶切割克隆来破坏靶基因的方法，以显示与常规方法相比提高的准确性，方便性和增加的转化效率，从而可用于遗传工程和生物工业。 在通过将位于5'末端的上部的核苷酸序列片段（a）的核苷酸序列片段（c）插入目标框架的开放的起始帧起始点的方法来破坏靶基因的方法， 序列片段（b）从选择性标记的开放引导帧起始点到选择性标记的开放引导帧起始点的内部的某个点从5'末端侧开始; 以及核苷酸片段（f），其包含起始点的核苷酸序列片段（d），其与核苷酸序列片段（b）的重叠部分与选择性标记的开放的前端结束点和核苷酸序列片段 （e）从5'末端侧依次位于靶基因的开放引导框架终点的3'末端的下位置，宿主核苷酸序列片段（c）由核苷酸序列片段（ a）通过限制酶位点插入，并且从选择性标记的全部基因序列和包含核苷酸的载体（B）获得包含选择性标记的所有基因序列和核苷酸序列片段（f）的载体（A） 通过限制酶位点插入的序列片段（e）。

公开(公告)号：KR100452082B1

公开(公告)日：2004-10-08

申请号：KR1020010065808

申请日：2001-10-24

Applicant: 재단법인서울대학교산학협력재단 ,  주식회사 바이오앤진 ,  주식회사 보락

Inventor： 서진호 ,  고영호 ,  김명동 ,  정수련 ,  김상용

IPC: C12Q1/68

Abstract: PURPOSE: Provided are a method of quantitatively and qualitatively analyzing lactic acid bacteria and a PCR primer which is capable of quantitatively and qualitatively analyzing lactic acid bacteria present in fermented foods. The quantitative and qualitative analysis can be carried out by performing QC-PCR with a PlaF/PlaR primer and an MesF/MesR primer to determine the fermentation degree and aging degree of fermented foods, and the storage state and quality thereof. CONSTITUTION: A method for quantitatively analyzing lactic acid bacteria comprises the step of: adding a target DNA to a sample and mixing a competitor Cmes or Cpla and performing PCR with a specific primer set of Lactobacillus plantarum or a specific primer set of Leuconostoc mesenteroides; measuring PCR amplification of the competitor and target DNA; and calculating the concentration of the target DNA concentration from the PCR amplification rate and the concentration of the competitor.

Abstract translation: 目的：提供定量和定性分析乳酸菌的方法和能够定量和定性分析发酵食品中存在的乳酸菌的PCR引物。 定量和定性分析可以通过使用PlaF / PlaR引物和MesF / MesR引物进行QC-PCR来确定发酵食品的发酵程度和老化程度，以及其储存状态和质量。 构成：定量分析乳酸菌的方法包括以下步骤：向样品中加入靶DNA并混合竞争者Cmes或Cpla，并用特定的植物乳杆菌（Lactobacillus plantarum）引物组或特定的Leuconostoc mesenteroide引物组进行PCR; 测量竞争者和靶DNA的PCR扩增; 并根据PCR扩增速率和竞争者的浓度计算目标DNA浓度的浓度。