公开(公告)号：KR100462805B1

公开(公告)日：2004-12-29

申请号：KR1020020011883

申请日：2002-03-06

Applicant: 재단법인서울대학교산학협력재단

Inventor： 남중희 ,  권영기 ,  강경돈 ,  이기훈

IPC: C12Q1/37

Abstract: PURPOSE: A method for the enzyme hydrolysis of sericine and a reactor used therefor are provided, therefore the high purity, low molecular weight sericine can be obtained and used enzyme can be reused, so that the low molecular weight sericine can be cheaply obtained. CONSTITUTION: The method for the enzyme hydrolysis of sericine comprises the steps of: partially hydrolyzing a fiber to expose hydroxyl group (-OH) and carboxyl group(-COOH) on the surface of the fiber; activating the surface of the fiber using a cross-linking agent; fixing an enzyme on the activated surface of the fiber; loading the fiber in two separate enzyme reactors; and circulating a sericine solution into the enzyme reactors to decompose the sericine, in which the fiber is polyester or nylon, the cross-linking agent is glutaraldehyde, and the enzyme is trypsin or alpha-chymotrypsin(wherein, the trypsin is fixed on a first enzyme reactor and the alpha-chymotrypsin is fixed on a second enzyme reactor).

Abstract translation: 目的：提供丝胶酶的酶水解方法及其所用的反应器，因此可以获得高纯度，低分子量的丝胶蛋白，并且可以将酶重新使用，从而可以便宜地获得低分子量丝胶蛋白。 构成：丝素酶水解的方法包括以下步骤：部分水解纤维以暴露纤维表面上的羟基（-OH）和羧基（-COOH）; 使用交联剂活化纤维的表面; 将酶固定在纤维的活化表面上; 将纤维装入两个分开的酶反应器中; 并将丝胶蛋白溶液循环到酶反应器中以分解丝胶，其中纤维是聚酯或尼龙，交联剂是戊二醛，酶是胰蛋白酶或α-胰凝乳蛋白酶（其中胰蛋白酶固定在第一 酶反应器并将α-胰凝乳蛋白酶固定在第二个酶反应器上）。

公开(公告)号：KR1020020026507A

公开(公告)日：2002-04-10

申请号：KR1020020011883

申请日：2002-03-06

Applicant: 재단법인서울대학교산학협력재단

Inventor： 남중희 ,  권영기 ,  강경돈 ,  이기훈

IPC: C12Q1/37

Abstract: PURPOSE: A method for the enzyme hydrolysis of sericine and a reactor used therefor are provided, therefore the high purity, low molecular weight sericine can be obtained and used enzyme can be reused, so that the low molecular weight sericine can be cheaply obtained. CONSTITUTION: The method for the enzyme hydrolysis of sericine comprises the steps of: partially hydrolyzing a fiber to expose hydroxyl group (-OH) and carboxyl group(-COOH) on the surface of the fiber; activating the surface of the fiber using a cross-linking agent; fixing an enzyme on the activated surface of the fiber; loading the fiber in two separate enzyme reactors; and circulating a sericine solution into the enzyme reactors to decompose the sericine, in which the fiber is polyester or nylon, the cross-linking agent is glutaraldehyde, and the enzyme is trypsin or alpha-chymotrypsin(wherein, the trypsin is fixed on a first enzyme reactor and the alpha-chymotrypsin is fixed on a second enzyme reactor).

Abstract translation: 目的：提供丝氨酸酶水解方法和用于其的反应器的方法，因此可以获得高纯度，低分子量的丝氨酸，并且可以重复使用酶，从而可以廉价地获得低分子量的丝氨酸。 构成：丝氨酸酶水解的方法包括以下步骤：部分水解纤维以暴露纤维表面的羟基（-OH）和羧基（-COOH）; 使用交联剂活化纤维的表面; 将酶固定在纤维的活化表面上; 将纤维加载到两个单独的酶反应器中; 并将丝氨酸溶液循环到酶反应器中以分解其中纤维是聚酯或尼龙的丝氨酸，交联剂是戊二醛，酶是胰蛋白酶或α-糜蛋白酶（其中，胰蛋白酶固定在第一 酶反应器和α-胰凝乳蛋白酶固定在第二酶反应器上）。