公开(公告)号：KR100781062B1

公开(公告)日：2007-11-30

申请号：KR1020060120416

申请日：2006-12-01

Applicant: 재단법인서울대학교산학협력재단

Inventor： 정종주 ,  김정호 ,  최양도 ,  서주석

IPC: C12N9/24 ,  C12N9/14

Abstract: A trehalose synthesizing fusion protein is provided to synthesize and hydrolyze maltooligosyltrehalose and increase the production efficiency of trehalose due to excellent heat-resistance. A maltooligosyltrehalose synthesis hydrolase(MhMTSHase) protein derived from Metallosphaera hakonesis(JCM8857) includes an amino acid sequence of SEQ ID : NO. 2. An MhMTSH gene encodes the MhMTSHase protein and includes a sequence of SEQ ID : NO. 1. A method for producing the protein comprises a step of culturing E. coli transformed by a recombinant vector including the MhMTSH gene. A method for producing trehalose comprises a step of adding substrate such as a water-soluble starch or a liquid corn starch to the MhMTSHase protein and then reacting it at a temperature of 65-75 deg.C under the pH of 5.0-6.0.

Abstract translation: 提供海藻糖合成融合蛋白以合成和水解麦芽糖基海藻糖，并且由于优异的耐热性而提高海藻糖的生产效率。 衍生自金丝酵母属（JCM8857）的麦芽寡糖基海藻糖合成水解酶（MhMTSHase）蛋白包含SEQ ID NO： MhMTSH基因编码MhMTSHase蛋白，并包括SEQ ID NO： 1.一种生产蛋白质的方法包括培养由包含MhMTSH基因的重组载体转化的大肠杆菌的步骤。 海藻糖的制造方法包括向MhMTSHase蛋白质添加水溶性淀粉或液体玉米淀粉等底物，然后在5.0〜6.0的pH下，在65-75℃的温度下进行反应。