公开(公告)号：KR1020100001907A

公开(公告)日：2010-01-06

申请号：KR1020080061997

申请日：2008-06-27

Applicant: 재단법인서울대학교산학협력재단

Inventor： 유현주 ,  박세진 ,  박명수 ,  지근억

IPC: A61K31/7048 ,  A61K36/062

CPC classification number: A61K31/7048 ,  A61K36/062

Abstract: PURPOSE: A method for preparing quercetin glucoside from rutin using rhamnosidase(EC 3.2.1.40) is provided to improve productivity of quercetin-3-glucoside having apoptosis ability to cancer cells. CONSTITUTION: A quercetin-3-glucoside having anticancer activity is converted from Aspergillus niger(KCTC 6906)-derived rhamnosidase. A method for isolating the rhamnosidase comprises: a step of shaking Aspergillus niger(KCTC 6906) in PD(potato dextrose) and ME(malt extract) at 30°C for five days; a step of filtering the culture medium using Whatman Glass Microfibre filter; a step of filtering again with bottle top filter at 0.22μm; a step of dissolving the filtered liquid in 85% ammonium sulfate, centrifuging to remove supernatant, and dissolving precipitate in buffer; and a step of isolating through DEAE-cellulose ion chromatography sephacryl S-200 gel chromatography.

Abstract translation: 目的：提供使用鼠李糖苷酶（EC 3.2.1.40）从芦丁制备槲皮素葡萄糖苷的方法，以提高具有对癌细胞凋亡能力的槲皮素-3-葡萄糖苷的生产力。 构成：具有抗癌活性的槲皮素-3-葡萄糖苷由黑曲霉（KCTC 6906）衍生的鼠李糖苷酶转化。 分离鼠李糖苷酶的方法包括：在30℃下在PD（马铃薯葡萄糖）和ME（麦芽提取物）中摇动黑曲霉（KCTC 6906）5天的步骤; 使用Whatman Glass Microfibre过滤器过滤培养基的步骤; 用0.22μm的瓶顶过滤器再次过滤; 将过滤的液体溶解在85％硫酸铵中的步骤，离心除去上清液，并将沉淀物溶解在缓冲液中; 并通过DEAE-纤维素离子色谱Sephacryl S-200凝胶色谱分离步骤。