公开(公告)号：KR100777232B1

公开(公告)日：2007-11-28

申请号：KR1020060119228

申请日：2006-11-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이인원 ,  이예련 ,  윤성환

IPC: C12N1/14

Abstract: A strain for mass-producing zearalenone is provided to increase the productivity of the zearalenone compared to conventional strains significantly. And a method for mass-producing the zearalenone through liquid or solid culturing the strain is provided to produce the zearalenone at low cost with high efficiency. A Gibberella zeae for mass-producing zearalenone is deposited as a deposition no. KCCM 10796P. A method for increasing the zearalenone production comprises a step of overexpressing ZEB2 in a strain in which ZEB1 is eliminated in zearalenone production pathways of the Gibberella zeae. A method for mass-producing the zearalenone comprises a step of culturing the strain in a solid medium.

Abstract translation: 提供了用于批量生产玉米赤霉烯酮的菌株，以显着提高玉米赤霉烯酮的生产力。 提供通过液体或固体培养菌株大量生产玉米赤霉烯酮的方法，以低成本高效率生产玉米赤霉烯酮。 用于批量生产玉米赤霉烯酮的玉米赤霉菌被沉积为沉积物。 KCCM 10796P。 增加玉米赤霉烯酮生产的方法包括在玉米赤霉菌的玉米赤霉烯酮生产途径中消除ZEB1的菌株中过量表达ZEB2的步骤。 用于批量生产玉米赤霉烯酮的方法包括在固体培养基中培养菌株的步骤。

公开(公告)号：KR100777231B1

公开(公告)日：2007-11-28

申请号：KR1020060119227

申请日：2006-11-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이인원 ,  이예련 ,  윤성환

IPC: C12N1/16 ,  C12N1/00

Abstract: A beta- zearalenol mass-producing strain is provided to increase the productivity of the beta-zearalenol compared to conventional strains significantly. And a method for mass-producing the beta-zearalenol through liquid or solid culturing the strain is provided to produce the beta-zearalenol efficiently at low cost. A Gibberella zeae mass-producing beta-zearalenol is deposited as a deposition no. KCCM 10795P. A method for increasing the beta-zearalenol production is characterized in that ZEB2 is overexpressed in a strain in which ZEB1 is eliminated in zearalenone production pathways. A method for mass-producing the beta-zearalenol is characterized in that the Gibberella zeae is cultured on a solid medium.

Abstract translation: 提供了一种β-谷甾烯醇大量生产菌株，以显着提高β-玉米赤霉烯醇的生产力。 提供了通过液体或固体培养菌株大量生产β-玉米赤霉烯醇的方法，以低成本有效地生产β-玉米赤霉烯醇。 玉米赤霉素大量生产β-玉米赤霉烯醇作为沉积物沉积。 KCCM 10795P。 增加β-玉米赤霉烯醇生产的方法的特征在于ZEB2在玉米赤霉烯酮生产途径中消除ZEB1的菌株中过表达。 用于大规模生产β-玉米赤霉烯醇的方法的特征在于将赤霉病菌在固体培养基上培养。

公开(公告)号：KR100777229B1

公开(公告)日：2007-11-28

申请号：KR1020060119226

申请日：2006-11-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이인원 ,  정선요 ,  김정은 ,  윤성환

IPC: C12N1/14

Abstract: A method for economically mass-producing zearalenone is provided to obtain large amount of the zearalenone by removing a PKS 12 which synthesizes aurofusarin in the production pathways of the zearalenone. A Gibberella zeae mass-producing zearalenone is deposited as a deposition no. KCCM 10794P. A method for increasing the zearalenone production comprises a step of blocking the aurofusarin biosynthesis pathway of the Gibberella zeae by deleting a PKS12 gene. A method for mass-producing the zearalenone comprises a step of solid-culturing the strain.

Abstract translation: 提供了一种经济地生产玉米赤霉烯酮的方法，通过去除在玉米赤霉烯酮生产途径中合成香叶素的PKS 12来获得大量的玉米赤霉烯酮。 玉米赤霉菌大量生产玉米赤霉烯酮作为沉积物沉积。 KCCM 10794P。 增加玉米赤霉烯酮生产的方法包括通过缺失PKS12基因来阻断玉米赤霉病的aurofusarin生物合成途径。 批量生产玉米赤霉烯酮的方法包括固定培养菌株的步骤。