公开(公告)号：KR1020090033701A

公开(公告)日：2009-04-06

申请号：KR1020070098866

申请日：2007-10-01

Applicant: 재단법인서울대학교산학협력재단

Inventor： 이진 ,  남현 ,  이재천

IPC: C12N5/07 ,  C12N5/02 ,  A61M39/02

Abstract: A method for primarily cultivating cells using frozen tissue is provided to facilitate animal cell culture by enabling an operator to massively secure cells to be primarily cultivated in a desired time and place. A method for primarily cultivating cells using frozen tissue comprises the following steps of: rapidly defrosting the frozen tissue at a temperature range of 35-38°C; treating the frozen tissue with proteinase before the frozen tissue is completely defrosted or making the frozen tissue into small pieces; and cultivating the cells. A method for freezing tissue has the following steps of: cutting off animal tissue, dipping the animal tissue in a cryopreservation liquid and gradually freezing the animal tissue for 6-48 hours to a temperature range of -50~-100°C; and cryopreserving the frozen tissue in liquid nitrogen. The cryopreservation liquid contains blood serum and anti-freezer.

Abstract translation: 提供了使用冷冻组织主要培养细胞的方法，以通过使操作者能够在期望的时间和地点大量地确保要主要培养的细胞来促进动物细胞培养。 使用冷冻组织主要培养细胞的方法包括以下步骤：在35-38℃的温度范围内快速除霜冷冻组织; 在冷冻组织完全解冻之前用蛋白酶处理冷冻组织，或将冷冻的组织制成小块; 并培养细胞。 冷冻组织的方法有以下步骤：切断动物组织，将动物组织浸入冷冻保存液中，逐渐将动物组织冷冻6-48小时至-50〜-100℃的温度范围; 并冷冻保存在液氮中的组织。 冷冻保存液含有血清和抗冻剂。