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公开(公告)号：KR101010284B1
公开(公告)日：2011-01-24
申请号：KR1020070066935
申请日：2007-07-04
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: A61L27/227 , A61K38/39 , A61L27/34 , A61L2430/02 , C07K14/78 , C07K2319/00 , C08L89/00
Abstract: 본 발명은 PHSRN-RGD 포함 올리고펩타이드를 유효성분으로 함유하는 골형성 촉진용 조성물에 관한 것으로, 더욱 구체적으로 서열번호 1 및
서열번호 2
사이에 펩타이드 링커(서열번호 3)를 포함하는 골형성 촉진용 조성물에 관한 것이다. 본 발명의 조성물은 골아세포의 부착 및 분화를 유의하게 증가시키며, 골재생능력을 향상시키므로 골이식재 또는 조직공학용 지지체 등에 상기 조성물을 고정시켜 원하는 국소에 투여하여 골형성을 촉진하는데 유용하게 이용될 수 있다.
세포부착, 세포외 기질, 피브로넥틴(Fibronectin), 골아세포(Osteoblast)-
公开(公告)号：KR1020090033701A
公开(公告)日：2009-04-06
申请号：KR1020070098866
申请日：2007-10-01
Applicant: 재단법인서울대학교산학협력재단
Abstract: A method for primarily cultivating cells using frozen tissue is provided to facilitate animal cell culture by enabling an operator to massively secure cells to be primarily cultivated in a desired time and place. A method for primarily cultivating cells using frozen tissue comprises the following steps of: rapidly defrosting the frozen tissue at a temperature range of 35-38°C; treating the frozen tissue with proteinase before the frozen tissue is completely defrosted or making the frozen tissue into small pieces; and cultivating the cells. A method for freezing tissue has the following steps of: cutting off animal tissue, dipping the animal tissue in a cryopreservation liquid and gradually freezing the animal tissue for 6-48 hours to a temperature range of -50~-100°C; and cryopreserving the frozen tissue in liquid nitrogen. The cryopreservation liquid contains blood serum and anti-freezer.
Abstract translation: 提供了使用冷冻组织主要培养细胞的方法，以通过使操作者能够在期望的时间和地点大量地确保要主要培养的细胞来促进动物细胞培养。 使用冷冻组织主要培养细胞的方法包括以下步骤：在35-38℃的温度范围内快速除霜冷冻组织; 在冷冻组织完全解冻之前用蛋白酶处理冷冻组织，或将冷冻的组织制成小块; 并培养细胞。 冷冻组织的方法有以下步骤：切断动物组织，将动物组织浸入冷冻保存液中，逐渐将动物组织冷冻6-48小时至-50〜-100℃的温度范围; 并冷冻保存在液氮中的组织。 冷冻保存液含有血清和抗冻剂。
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公开(公告)号：KR1020100006452A
公开(公告)日：2010-01-19
申请号：KR1020080066683
申请日：2008-07-09
Applicant: 재단법인서울대학교산학협력재단
Abstract: PURPOSE: A method for culturing human embryonic stem cells using human umbilical cord-derived fibroblast is provided to maintain undifferentiation and pluripotency improve medical usefulness. CONSTITUTION: A human embryonic stem cell is cultured by co-culturing human embryonic stem cells with umbilical cord-derived fibroblast. The cell division of fibroblasts is suppressed using mitomycin C treatment or gamma irradiation when the growth rate of the fibroblasts is 60-80%.
Abstract translation: 目的：提供使用人脐带来源的成纤维细胞培养人胚胎干细胞的方法，以保持未分化和多能性提高医学用途。 构成：通过用脐带来源的成纤维细胞共培养人胚胎干细胞培养人胚胎干细胞。 当成纤维细胞的生长速率为60-80％时，使用丝裂霉素C处理或γ照射来抑制成纤维细胞的细胞分裂。
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公开(公告)号：KR1020090002736A
公开(公告)日：2009-01-09
申请号：KR1020070066935
申请日：2007-07-04
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: A61L27/227 , A61K38/39 , A61L27/34 , A61L2430/02 , C07K14/78 , C07K2319/00 , C08L89/00
Abstract: A composition for promoting the osteogenesis, and a bone graft material coated with the composition are provided to improve the bone regeneration by increasing the differentiation of osteoblast. A composition for promoting the osteogenesis contains an oligopeptide comprising A-L-B as an active ingredient, wherein A is PHSRN, B is RGD, and L is a linker peptide comprising 15~25 amino acids. Preferably the oligopeptide contains a peptide linker between the amino acid represented by the sequence number 1 and the amino acid represented by the sequence number 2.
Abstract translation: 提供促进成骨的组合物和涂覆有组合物的骨移植材料，以通过增加成骨细胞的分化来改善骨再生。 用于促进成骨的组合物含有包含A-L-B作为活性成分的寡肽，其中A是PHSRN，B是RGD，L是包含15〜25个氨基酸的接头肽。 优选地，寡肽在由序列号1表示的氨基酸和由序列号2表示的氨基酸之间含有肽接头。
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