公开(公告)号：KR1020080093691A

公开(公告)日：2008-10-22

申请号：KR1020070037767

申请日：2007-04-18

Applicant: 재단법인서울대학교산학협력재단

Inventor： 남좌민 ,  장경진

IPC: C12Q1/00

CPC classification number: G01N33/5008 ,  C12N5/0068 ,  C12N2533/12 ,  C12N2535/00 ,  G01N33/54306

Abstract: A cell-assay method is provided to form various nano-structured matrices for cell culturing similar to in vivo environment easily and analyze influence of various structured three-dimensional surface and functional materials on cells on a chip. A cell-assay method comprises the steps of: (a) arraying nano-particles using a piezo-microarrayer on a glass slide; (b) arraying a functional material using the piezo-microarrayer on a nano-array obtained from the step(a) to modify the nano-particles; and (c) culturing cells on the surface of the nano-array modified by the functional material and analyzing them, wherein the nano-particle is at least one selected from the group consisting of gold nanosphere, gold nanotriangle, gold nanocube, gold nanotetrapod, gold nanohexagon, silver nanosphere, silver nanotriangle and a mixture thereof and the functional material is at least one selected from the group consisting of extracellular matrix(ECM) proteins, medicines for treatment, medicines for diagnosis, and growth factors. Additionally, a step of surface -treating a glass slide with a coating agent selected from silane-polyethyleneglycol and poly(L-lysine)-g-polyethyleneglycol before the step (a) is comprised in the cell-assay method.

Abstract translation: 提供细胞测定方法，容易地形成用于细胞培养的各种纳米结构基质，并分析各种结构的三维表面和功能材料对芯片上的细胞的影响。 细胞分析方法包括以下步骤：（a）使用压电微阵列器在载玻片上排列纳米颗粒; （b）使用压电微阵列器在由步骤（a）获得的纳米阵列上排列功能材料以修饰纳米颗粒; 和（c）在由功能材料修饰的纳米阵列的表面上培养细胞并对其进行分析，其中纳米颗粒是选自金纳米球，金纳米三角，金纳米管，金纳米四面体， 金纳米六角珠，银纳米球，银纳米三角及其混合物，功能材料为选自细胞外基质（ECM）蛋白，治疗药物，诊断用药物和生长因子中的至少一种。 此外，在步骤（a）之前，用选自硅烷 - 聚乙二醇和聚（L-赖氨酸）-g-聚乙二醇的涂层剂在玻璃载片上表面起作用的步骤包括在细胞测定方法中。