백수오와 이엽우피소의 감별 방법 및 그 키트
    1.
    发明公开
    백수오와 이엽우피소의 감별 방법 및 그 키트 有权
    用于分离CYNANCHUM WILFORDII和CYNANCHUM AURICULATUM及其制品的方法

    公开(公告)号:KR1020120062614A

    公开(公告)日:2012-06-14

    申请号:KR1020110109104

    申请日:2011-10-25

    CPC classification number: C12Q1/683 C12Q1/6858 C12Q2600/124 C12Q2600/156

    Abstract: PURPOSE: A method for distinguishing Cynanchum wilfordii and Cynanchum auriculatum is provided to enable distinguishing Cynanchum wilfordii and Cynanchum auriculatum by direct sequencing, SNP genotyping, and RFLP. CONSTITUTION: A method for distinguishing Cynanchum wilfordii and Cynanchum auriculatum using RFLP analysis comprises a step of preparing a biological sample from Cynanchum wilfordii and Cynanchum auriculatum; a step of isolating genome DNA from the biological sample; a step of amplifying DNS sequence having a sequence of sequence number 1 and amplifying DNA sequence of sequence number 2 of Cynanchum auriculatum; and a step of treating the amplified products with Xcml and detecting the cleaved DNA fragment.

    Abstract translation: 目的:通过直接测序,SNP基因分型和RFLP分析Cynanchum wilfordii和Cynanchum auriculatum的方法,以区分Cynanchum wilfordii和Cynanchum auriculatum。 构成:使用RFLP分析来区分枸杞子和Cynanchum auriculatum的方法包括从Cynanchum wilfordii和Cynanchum auriculatum制备生物样品的步骤; 从生物样品中分离基因组DNA的步骤; 扩增具有序列号1的序列的DNS序列并扩增Cynanchum auriculatum的序列号2的DNA序列的步骤; 以及用Xcml处理扩增产物并检测切割的DNA片段的步骤。

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