Abstract:
PURPOSE: A method for distinguishing Cynanchum wilfordii and Cynanchum auriculatum is provided to enable distinguishing Cynanchum wilfordii and Cynanchum auriculatum by direct sequencing, SNP genotyping, and RFLP. CONSTITUTION: A method for distinguishing Cynanchum wilfordii and Cynanchum auriculatum using RFLP analysis comprises a step of preparing a biological sample from Cynanchum wilfordii and Cynanchum auriculatum; a step of isolating genome DNA from the biological sample; a step of amplifying DNS sequence having a sequence of sequence number 1 and amplifying DNA sequence of sequence number 2 of Cynanchum auriculatum; and a step of treating the amplified products with Xcml and detecting the cleaved DNA fragment.
inflexus ) 추출물을 유효성분으로 함유하는 미백용 화장료조성물에 관한 것이다. 또한, 본 발명은 산박하 추출물로부터 멜라닌생성 억제작용을 갖는 화학식 1로 표시되는 excisanin A 화합물을 분리 정제하는 방법을 제공한다. 산박하추출물, 미백, excisanin A
Abstract:
A cosmetic composition and a manufacturing method of the solvent fraction of the extracts of Distylium racemosum are provided to have excellent antioxidant effect, whitening effect and skin wrinkle generation suppressive effect. A cosmetic composition comprises a solvent fraction of extracts of Distylium racemosum or the extracts of Distylium racemosum as active ingredient. An active component of the solvent fraction of the extracts of Distylium racemosum or the extracts of Distylium racemosum is indicated as a chemical formula 1. A manufacturing method of the solvent fraction of the extracts of Distylium racemosum comprises steps of: extracting over 1 time it by dipping stem and leaf of the Distylium racemosum into a mixed solution of water and methanol or ethanol; obtaining methanol extraction or ethanol extraction by concentrating the obtained extracts; obtaining a hexane graduation layer by suspending the methanol extraction or the ethanol extraction into 20% methanol or ethanol and extracting it with hexane 3 times; and obtaining a solvent fractionation by adding ethylacetate and butanol successively and extracting it 3 times.
var . canescens ) 추출물을 유효성분으로 함유하는 미백 및 항산화효능을 갖는 화장료조성물에 관한 것이다. 또한 본 발명은 털산박하 추출물로부터 멜라닌생성 억제작용을 갖는 화학식 1로 표시되는 kamebakaurin 화합물을 분리 정제하는 방법을 제공한다. 털산박하추출물, 항산화, 미백, kamebakaurin
Abstract:
A cosmetic composition for antioxidation and whitening is provided to have the effect of suppressing melanogenesis by isolating kamebakaurin which is an active ingredient. A composition for antiaging and whitening comprises Isodon inflexus Var. Canesens extract or its solvent fraction as an active ingredient. The content of extract or solvent fraction is 0.00001-10 weight% based on total weight of the composition. A method for producing solvent fraction of Isodon inflexus Var. Canesens extract comprises: a step of dipping Isodon inflexus Var. Canesens plant with water and ethanol or methanol mixture solution and extracting once more to obtain ethanol or methanol extract; a step of suspending the extrac in 20% of ethanol or methanol and extracting three times to obtain hexane fraction layer; and a step of adding ethylacetate and butanol and to obtain solvent fraction.
Abstract:
본 발명은 차풀( Cassia mimosoides var. nomame )추출물의 제조방법 및 차풀 추출물을 유효성분으로 함유하는 피부노화방지 및 피부주름방지용 화장료조성물에 관한 것이다. 차풀 추출물에는 항산화물질인 화학식 1로 표시되는 루테오린 화합물을 포함하고 있으며, 이 루테오린 화합물이 항산화 및 항주름 효능을 포함한 피부노화방지효과를 나타낸다. 또한, 본 발명은 차풀 추출물로부터 루테오린의 분리·정제방법을 제공한다. 본 발명의 차풀 추출물에 함유되어 있는 루테오린화합물은 엘라스타아제 저해작용, mmp-1 저해작용, 라디칼 포착형 항산화작용등을 갖고 있다. 차풀 추출물, 루테오린, 항산화, 피부노화방지, 화장료
Abstract:
본 발명은 백수오 및 이엽우피소의 감별 방법 및 그 키트에 관한 것이다. 본 발명의 백수오 및 이엽우피소의 감별 방법 및 그 키트는 백수오의 ITS에 존재하는 Xcm I 제한효소 인식 부위와 이에 대한 이엽우피소의 대립 서열을 이용하거나 백수오와 이엽우피소의 ITS 서열을 이용함을 특징으로 한다.
Abstract:
A cosmetic and skin external composition of whitening and anti-inflammatory effect is provided to suppress the mRNA expression of genes which are related to melanogenesis, NO generation, inflammation using a henryin. A cosmetic composition for whitening and anti-inflammation comprises a henryin as an active ingredient. The henryin is contained in a ratio of 0.00001-10 weight% based on the total weight. A composition for skin external agent comprises 0.00001-10 weight% henryin as an active ingredient. The cosmetic and skin external agent for whitening and anti-inflammation is used in a form of cream, lotion, essence, and pack.