公开(公告)号：KR101154323B1

公开(公告)日：2012-06-13

申请号：KR1020110107874

申请日：2011-10-21

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  김주열 ,  김원용

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/29

Abstract: PURPOSE: A transgenic plant with low temperature resistance or freezing tolerance is provided to ensure excellent germination rate at low temperature. CONSTITUTION: A plant with low temperature resistance or freezing tolerance is prepared by transforming a plant with a recombinant vector containing Oriza sativa-derived OsGRP(Oryza sativa glycine-rich RNA-binding protein) gene with a base sequence of sequence number 6. The plant is a dicotyledonous plant or mono-cotyledonous plant. The plant is Arabidopsis thaliana. A method for producing the plant comprises: a step of transforming a plant cells with the recombinant vector; and a step of redifferentiating the transgenic plant.

公开(公告)号：KR1020110122660A

公开(公告)日：2011-11-10

申请号：KR1020110107874

申请日：2011-10-21

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  김주열 ,  김원용

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/29

CPC classification number: C12N15/8273 ,  A01H4/008 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8216

Abstract: PURPOSE: A transgenic plant with low temperature resistance or freezing tolerance is provided to ensure excellent germination rate at low temperature. CONSTITUTION: A plant with low temperature resistance or freezing tolerance is prepared by transforming a plant with a recombinant vector containing Oriza sativa-derived OsGRP(Oryza sativa glycine-rich RNA-binding protein) gene with a base sequence of sequence number 6. The plant is a dicotyledonous plant or mono-cotyledonous plant. The plant is Arabidopsis thaliana. A method for producing the plant comprises: a step of transforming a plant cells with the recombinant vector; and a step of redifferentiating the transgenic plant.

Abstract translation: 目的：提供耐低温或耐冷冻的转基因植物，以确保低温下优秀的发芽率。 构成：通过用含有来自序列号为6的碱基序列的含有来自Oriza sativa的OsGRP（富含谷氨酸甘氨酸富含RNA结合蛋白）基因的重组载体转化植物来制备耐温性或耐寒性的植物。植物 是双子叶植物或单子叶植物。 该植物是拟南芥。 一种生产该植物的方法包括：用重组载体转化植物细胞的步骤; 以及重新分化转基因植物的步骤。

公开(公告)号：KR101131600B1

公开(公告)日：2012-03-30

申请号：KR1020090116374

申请日：2009-11-30

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  김주열 ,  김원용

IPC: C12N15/82 ,  C12N15/29 ,  A01H1/00

Abstract: 본 발명은 벼에서 유래한 RNA-결합 단백질 (OsGRP)을 암호화하는 유전자를 이용하여 식물에 저온 또는 동결 내성을 부여하는 방법 및 이 방법에 의하여 제조된 형질전환 식물체에 관한 것이다. 본 발명에서 제작한 식물체는 저온에서 탁월한 발아율 및 유모 생장을 나타내며, 동결 조건에서 우수한 생존율을 보여, 본 발명은 저온 및 동결 내성 작물을 생산하는데 이용될 수 있다.
벼 RNA-결합 단백질, OsGRP, 저온 내성, 동결 내성, 형질전환, 식물

公开(公告)号：KR101154322B1

公开(公告)日：2012-06-13

申请号：KR1020110107871

申请日：2011-10-21

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  김주열 ,  김원용

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/29

Abstract: PURPOSE: A method for enhancing low temperature resistance or freezing tolerance using a gene encoding RNA-binding protein(OsGRP) is provided to ensure excellent germination rate at low temperature. CONSTITUTION: A plant with low temperature resistance or freezing tolerance is prepared by a recombinant vector containing Oriza sativa-derived OsGRP(Oryza sativa glycine-rich RNA-binding protein) gene with a base sequence of sequence number 1. The plant is Arabidopsis thaliana. A method for producing the plant with low temperature resistance or freezing tolerance comprises: a step of transforming a plant cells with the recombinant vector; and a step of redifferentiating a transgenic plant form the transformed plant cells.

公开(公告)号：KR1020110122659A

公开(公告)日：2011-11-10

申请号：KR1020110107871

申请日：2011-10-21

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  김주열 ,  김원용

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/29

CPC classification number: C12N15/8273 ,  A01H4/008 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8216

Abstract: PURPOSE: A method for enhancing low temperature resistance or freezing tolerance using a gene encoding RNA-binding protein(OsGRP) is provided to ensure excellent germination rate at low temperature. CONSTITUTION: A plant with low temperature resistance or freezing tolerance is prepared by a recombinant vector containing Oriza sativa-derived OsGRP(Oryza sativa glycine-rich RNA-binding protein) gene with a base sequence of sequence number 1. The plant is Arabidopsis thaliana. A method for producing the plant with low temperature resistance or freezing tolerance comprises: a step of transforming a plant cells with the recombinant vector; and a step of redifferentiating a transgenic plant form the transformed plant cells.

Abstract translation: 目的：提供使用编码RNA结合蛋白（OsGRP）的基因来提高耐低温性或耐寒性的方法，以确保低温下优秀的发芽率。 构成：具有耐温性或耐冷冻性的植物是通过含有基因序列为1的含有源自芸苔的OsGRP（富含谷氨酸甘氨酸富含RNA结合蛋白）基因的重组载体制备的。该植物是拟南芥。 一种生产具有耐低温性或耐冷冻性的植物的方法包括：用重组载体转化植物细胞的步骤; 以及将转基因植物重新分化形成转化的植物细胞的步骤。

公开(公告)号：KR1020110059927A

公开(公告)日：2011-06-08

申请号：KR1020090116374

申请日：2009-11-30

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  김주열 ,  김원용

IPC: C12N15/82 ,  C12N15/29 ,  A01H1/00

Abstract: PURPOSE: A method for producing a transgenic plant having cold and freezing tolerance is provided to ensure excellent germination rate. CONSTITUTION: A transgenic plant having cold and freezing tolerance is prepared by transforming with a recombinant vector containing Oriza sativa-derived glycine-rich RNA-binding protein(OsGRP) gene. The OsGRP gene has a base among sequence numbers 1-6. The plant is Arabidopsis thaliana. A composition with cold and freezing tolerance in a plant contains OsGRP gene.

Abstract translation: 目的：提供一种具有耐寒和耐寒耐性的转基因植物的生产方法，以确保优良的发芽率。 构成：具有冷和冷冻耐受性的转基因植物通过用含有富含芸苔的富含甘氨酸的谷氨酸RNA结合蛋白（OsGRP）基因的重组载体转化来制备。 OsGRP基因在序列号1-6之间具有碱基。 该植物是拟南芥。 植物中具有冷和耐寒性的组合物含有OsGRP基因。