公开(公告)号：KR101079041B1

公开(公告)日：2011-11-02

申请号：KR1020090032462

申请日：2009-04-14

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  곽경진 ,  장지영 ,  남영우

IPC: C12N15/11 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00

Abstract: 본발명은오이유래의카이네이스유전자의프로모터및 이를이용한형질전환식물체에관한것으로, 보다상세하게는오이유래의저온스트레스내성유도카이네이스유전자의프로모터, 상기프로모터를포함하는재조합발현벡터, 상기재조합발현벡터를이용한형질전환식물체의제조방법및 상기방법에의해제조된저온스트레스에대한내성이증가된형질전환식물체에관한것으로, 본발명에따른오이유래카이네이스유전자의프로모터와이를이용한저온스트레스저항성형질전환식물의품종개발기술은다양한경제작물의수확량증대에크게기여할수 있을것이다.

公开(公告)号：KR1020100113909A

公开(公告)日：2010-10-22

申请号：KR1020090032462

申请日：2009-04-14

Applicant: 전남대학교산학협력단

Inventor： 강훈승 ,  곽경진 ,  장지영 ,  남영우

IPC: C12N15/11 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00

Abstract: PURPOSE: A promoter of Cucumis sativas-derived kinase gene and transgenic plant using the same are provided to enhance yield of various crops. CONSTITUTION: A promoter of Cucumis staivas-derived kinase gene comprises nucleotide sequence of sequence number 1. A recombinant expression vector has the promoter. The recombinant expression vector additionally contains a gene at downstream, encoding a target protein. The transformed microorganism is E.coli or Agrobacterium tumefaciens which is transformed with the recombinant expression vector. A transgenic plant is produced by transforming with the transformed microorganism. The transgenic plant has low temperature stress resistance. The plant is Arabidopsis thaliana.

Abstract translation: 目的：提供使用其的Cucumis Sativas衍生的激酶基因的启动子和转基因植物以提高各种作物的产量。 构成：Cucumis staivas来源的激酶基因的启动子包含序列号1的核苷酸序列。重组表达载体具有启动子。 重组表达载体另外在下游含有编码靶蛋白的基因。 转化的微生物是用重组表达载体转化的大肠杆菌或根癌土壤杆菌。 通过用转化的微生物转化来产生转基因植物。 转基因植物具有低温胁迫性。 该植物是拟南芥。