公开(公告)号：KR100831071B1

公开(公告)日：2008-05-20

申请号：KR1020070008514

申请日：2007-01-26

Applicant: 전남대학교산학협력단

Inventor： 이기영 ,  박정순 ,  정경환 ,  황현수

IPC: G01N33/569 ,  G01N33/48 ,  G01N33/53

Abstract: A method for detecting Streptococcus pneumoniae or Staphylococcus aureus in a sample is provided to detect the Streptococcus pneumoniae or Staphylococcus aureus accurately and very simply within a short time and also measure concentration of the Streptococcus pneumoniae or Staphylococcus aureus, thereby being easy to determine the existence of the bacteria and the outbreak of diseases. A method for detecting Streptococcus pneumoniae in a sample comprises the steps of: (a) applying the sample onto a biosensor where a pneumococcal polysaccharide is fixed on the surface; and (b) measuring surface plasmon resonance caused by interaction between the pneumococcal polysaccharide and the sample. A method for detecting Staphylococcus aureus comprises the steps of: (a) applying the sample onto a biosensor where at least one material selected from the group consisting of protopectin, pectic acid, pectin acid and pectin is fixed on the surface; and (b) measuring surface plasmon resonance caused by interaction between the pneumococcal polysaccharide and the sample.

Abstract translation: 提供样本中检测肺炎链球菌或金黄色葡萄球菌的方法，在短时间内准确，简便地检测肺炎链球菌或金黄色葡萄球菌，并测定肺炎链球菌或金黄色葡萄球菌的浓度，从而易于确定 细菌和疾病的爆发。 检测样品中肺炎链球菌的方法包括以下步骤：（a）将样品施用于表面上固定肺炎球菌多糖的生物传感器上; 和（b）测量由肺炎球菌多糖与样品之间的相互作用引起的表面等离子体共振。 一种检测金黄色葡萄球菌的方法包括以下步骤：（a）将样品施加到生物传感器上，其中至少一种选自由原始果胶，果胶酸，果胶酸和果胶的材料固定在表面上; 和（b）测量由肺炎球菌多糖与样品之间的相互作用引起的表面等离子体共振。