Abstract:
With respect to the present invention, microorganisms which are endogeneous in Perinereis aibuhitensis known to have excellent capacity to decompose organic matters in the coastal mud flat were grown in an MA medium, and 31 colonies were selected according to the color and shape of the colony and then purely cultured. As a result of identification based on the 16S rRNA nucleotide sequence, 11 strains included in the genus Bacillus were isolated. As a result of analysis of diversity of 11 strains included in the genus Bacills, it was investigated that a total of seven species of Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, and Bacills vietnamensis are endogenous in Perinereis aibuhitensis. In the present invention, two Bacillus strains isolated and selected from Perinereis aibuhitensis have excellent capacity to decompose protein, hydrocarbon, and lipid, and have resistance to salt, and thus can be useful to purify a mud flat contaminated with organic matters.
Abstract:
In the present invention, a microorganism that inhabits a lugworm ( Perinereis aibuhitensis ) that is known for excellent organic material decomposition in shore mud flats is grown in an MA culture medium so that colonies occur. Thirty-one colonies are separately selected by color and shape in order to be purely cultured and identified based on a 16S rRNA base sequence, so that eleven strains included in the Bacills genus are separated. As a result of analyzing the variety of the eleven strains included in the Bacills genus, it has been investigated that seven Bacillus species, such as Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, and Bacills vietnamensis , inhabit the lugworm ( Perinereis aibuhitensis ). In the present invention, two Bacillus strains that have been separated and selected from the lugworm have excellent protein, carbohydrate, and lipid decomposition and salinity resistance so as to be usefully used to purify a mud flat environment that is contaminated by organic material.
Abstract:
본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이( Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과, Bacillus 속에 포함되는 11 균주를 분리하였다. Bacills 속(genus)에 포함되는 11 균주의 다양성을 분석한 결과 Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, Bacills vietnamensis 등 모두 7 종 (species)의 Bacillus spp.가 갯지렁이( Perinereis aibuhitensis )에 내생하는 것으로 조사되었다. 본 발명에서 갯지렁이로부터 분리하여 선별한 두 Bacillus 균주들은 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 갯벌 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.
Abstract:
본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이( Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과, Bacillus 속에 포함되는 11 균주를 분리하였다. Bacills 속(genus)에 포함되는 11 균주의 다양성을 분석한 결과 Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, Bacills vietnamensis 등 모두 7 종 (species)의 Bacillus spp.가 갯지렁이( Perinereis aibuhitensis )에 내생하는 것으로 조사되었다. 본 발명에서 갯지렁이로부터 분리하여 선별한 두 Bacillus 균주들은 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 갯벌 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.
Abstract:
The present invention isolates a microorganism with a bacterial name of EDB 2 in a coastal mud flat via enrichment and analyzes the characteristics of the microorganism that has excellent capacity for decomposing edosulfan and organic matters. As a result of the analysis of the physiobiochemical and genetic characteristics of the EDB2 bacterium, the EDB2 bacterium is 99% similar to Rhodococcus pyridinivorans. The EDB2 bacterium is aerobic, Gram-positive, bar-shaped and pink. The growth temperature range of the EDB2 bacterium is relatively wide as growability temperature ranges from 20 to 45°C and optimal temperature ranges from approximately 20 to 42°C. The EDB2 bacterium has strong possibility of being applied to purify lipid-polluted environment as the EDB2 bacterium does not have hydrolytic capacity for casein and cellulose but has decomposing capacity for DNA, agar, starch and tween, thus having excellent lipid-decomposing capacity. The EDB2 bacterium is acetoin-, urease-, α-glucosidase-, β-glucosidase-, mannitol-, malate-, alkalinephosphate-, esterase lipase-, leucine arylamidase-, trypsin-, acid phosphate-, esculin-, and sorbitol-positive.