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公开(公告)号：KR101475589B1
公开(公告)日：2014-12-22
申请号：KR1020130008983
申请日：2013-01-27
Applicant: 전남대학교산학협력단
Abstract: 본발명에서는연안갯벌에서농화배양을통해엔도설판및 유기물분해능이매우우수한미생물 (균주명 EDB 2)를분리하여그 특성을분석하였다. EDB2 균주에대한생리생화학적및 유전학적특성을분석한결과와 99%의높은상동성을보였다. 균주 EDB-2는호기성, 그람양성, 막대형이며, 핑크빛을띠고있다. 생장가능온도는 20℃~45℃, 최적온도는약 20℃~42℃로생장온도범위가비교적넓은것으로나타났다. Casein과 cellulose 가수분해능력은없지만 DNA, agar, starch 그리고 tween의분해능력이있는것으로나타나지질분해능이우수하여지질로오염된환경정화에응용가능성높은것으로나타났다. Acetoin, urease, α-glucosidase, &bgr;-glucosidase, mannitol, malate, alkaline phosphate, esterase lipase, leucine arylamidase, trypsin, acid phosphate, esculin, sorbitol 양성을보였다.
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Patent Agency Ranking2.发明公开
公开(公告)号：KR1020140073228A
公开(公告)日：2014-06-16
申请号：KR1020120141224
申请日：2012-12-06
Applicant: 전남대학교산학협력단
Abstract: With respect to the present invention, microorganisms which are endogeneous in Perinereis aibuhitensis known to have excellent capacity to decompose organic matters in the coastal mud flat were grown in an MA medium, and 31 colonies were selected according to the color and shape of the colony and then purely cultured. As a result of identification based on the 16S rRNA nucleotide sequence, 11 strains included in the genus Bacillus were isolated. As a result of analysis of diversity of 11 strains included in the genus Bacills, it was investigated that a total of seven species of Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, and Bacills vietnamensis are endogenous in Perinereis aibuhitensis. In the present invention, two Bacillus strains isolated and selected from Perinereis aibuhitensis have excellent capacity to decompose protein, hydrocarbon, and lipid, and have resistance to salt, and thus can be useful to purify a mud flat contaminated with organic matters.
Abstract translation: 对于本发明，在MA培养基中生长已知具有优异的分解沿海泥浆中的有机物质能力的围is it中的内分泌微生物，并根据菌落的颜色和形状选择31个菌落， 然后纯文化。 作为基于16S rRNA核苷酸序列的鉴定的结果，分离了芽孢杆菌属中包含的11个菌株。 通过分析芽孢杆菌属11种菌株的多样性，研究了七种芽孢杆菌，芽孢杆菌，芽孢杆菌，芽孢杆菌，百日咳芽孢杆菌，百日咳芽孢杆菌和越桔芽孢杆菌共有七种内源性 Per。 在本发明中，分离并选自百日香蓟马的两种芽孢杆菌菌株具有分解蛋白质，烃和脂质并具有耐盐性的优异能力，因此可用于纯化被有机物污染的泥浆。
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公开(公告)号：KR1020140062949A
公开(公告)日：2014-05-27
申请号：KR1020120129282
申请日：2012-11-15
Applicant: 전남대학교산학협력단
Abstract: In the present invention, a microorganism that inhabits a lugworm ( Perinereis aibuhitensis ) that is known for excellent organic material decomposition in shore mud flats is grown in an MA culture medium so that colonies occur. Thirty-one colonies are separately selected by color and shape in order to be purely cultured and identified based on a 16S rRNA base sequence, so that eleven strains included in the Bacills genus are separated. As a result of analyzing the variety of the eleven strains included in the Bacills genus, it has been investigated that seven Bacillus species, such as Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, and Bacills vietnamensis , inhabit the lugworm ( Perinereis aibuhitensis ). In the present invention, two Bacillus strains that have been separated and selected from the lugworm have excellent protein, carbohydrate, and lipid decomposition and salinity resistance so as to be usefully used to purify a mud flat environment that is contaminated by organic material.
Abstract translation: 在本发明中，在MA培养基中生长以在岸边泥浆平面中优异的有机物质分解为特征的居住在具有优良有机物分解的蛔虫（Perinereis aibuhitensis））的微生物，从而发生菌落。 通过颜色和形状分别选择三十一个菌落，以便基于16S rRNA碱基序列纯化培养和鉴定，从而分离包含在11个菌属中的11个菌株。 作为分析“芽孢杆菌”中包含的十一个菌株的多样性的结果，已经研究了七种芽孢杆菌属物种，例如细菌藻类，芽孢杆菌性溃疡 ，百日咳杆菌（Bacills aquimaris），芽孢杆菌（Bacills barbaricus），百日咳芽孢杆菌（Bacills hwajinpoensis），百日咳芽孢杆菌（Bacills nanhiensis）和越南小菜蛾（Bacills vietnamensis）居住在蛔虫（ Perinereis aibuhitensis ）。 在本发明中，已经从蛔虫中分离和选择的两种芽孢杆菌菌株具有优异的蛋白质，碳水化合物和脂质分解和耐盐性，从而有用地用于纯化平坦的 被有机材料污染。
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公开(公告)号：KR101414361B1
公开(公告)日：2014-07-01
申请号：KR1020120129282
申请日：2012-11-15
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 11 균주를 분리하였다.
Bacills 속(genus)에 포함되는 11 균주의 다양성을 분석한 결과
Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, Bacills vietnamensis 등 모두 7 종 (species)의
Bacillus spp.가 갯지렁이(
Perinereis aibuhitensis )에 내생하는 것으로 조사되었다. 본 발명에서 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주들은 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 갯벌 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.Abstract translation: 本发明涉及一种泻湖（泻湖）
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公开(公告)号：KR101447423B1
公开(公告)日：2014-10-06
申请号：KR1020120141224
申请日：2012-12-06
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 11 균주를 분리하였다.
Bacills 속(genus)에 포함되는 11 균주의 다양성을 분석한 결과
Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, Bacills vietnamensis 등 모두 7 종 (species)의
Bacillus spp.가 갯지렁이(
Perinereis aibuhitensis )에 내생하는 것으로 조사되었다. 본 발명에서 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주들은 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 갯벌 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.-
公开(公告)号：KR1020140096470A
公开(公告)日：2014-08-06
申请号：KR1020130008983
申请日：2013-01-27
Applicant: 전남대학교산학협력단
Abstract: The present invention isolates a microorganism with a bacterial name of EDB 2 in a coastal mud flat via enrichment and analyzes the characteristics of the microorganism that has excellent capacity for decomposing edosulfan and organic matters. As a result of the analysis of the physiobiochemical and genetic characteristics of the EDB2 bacterium, the EDB2 bacterium is 99% similar to Rhodococcus pyridinivorans. The EDB2 bacterium is aerobic, Gram-positive, bar-shaped and pink. The growth temperature range of the EDB2 bacterium is relatively wide as growability temperature ranges from 20 to 45°C and optimal temperature ranges from approximately 20 to 42°C. The EDB2 bacterium has strong possibility of being applied to purify lipid-polluted environment as the EDB2 bacterium does not have hydrolytic capacity for casein and cellulose but has decomposing capacity for DNA, agar, starch and tween, thus having excellent lipid-decomposing capacity. The EDB2 bacterium is acetoin-, urease-, α-glucosidase-, β-glucosidase-, mannitol-, malate-, alkalinephosphate-, esterase lipase-, leucine arylamidase-, trypsin-, acid phosphate-, esculin-, and sorbitol-positive.
Abstract translation: 本发明通过富集在沿海泥浆平板上分离细菌名称为EDB 2的微生物，并分析具有优异的分解能力的微生物的特性。 作为EDB2细菌的生理化学和遗传特征分析的结果，EDB2细菌与吡啶红霉素相似99％。 EDB2细菌是有氧的，革兰氏阳性，棒状和粉红色。 EDB2细菌的生长温度范围相对较宽，因为生长温度范围为20至45℃，最佳温度范围为约20至42℃。 由于EDB2细菌不具有酪蛋白和纤维素的水解能力，但对DNA，琼脂，淀粉和吐温具有分解能力，因此EDB2细菌具有很好的应用于净化脂质污染环境的可能性，具有优异的脂质分解能力。 EDB2细菌是乙偶姻，脲酶，α-葡萄糖苷酶，β-葡萄糖苷酶，甘露糖醇，苹果酸，磷酸 - 磷酸酯酶，酯酶脂肪酶，亮氨酸芳基酰胺酶，胰蛋白酶，酸式磷酸酯，esculin-和山梨糖醇 - 正。
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