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1.发明授权
公开(公告)号：KR101581998B1
公开(公告)日：2016-01-04
申请号：KR1020130032561
申请日：2013-03-27
Applicant: 전남대학교산학협력단
IPC: C02F3/34
Abstract: 본발명에서는연안갯벌에서농화배양을통해엔도설판및 유기물분해능이매우우수한미생물 (균주명 EDB 2)를분리하여생리생화학적및 유전학적특성을분석한결과와 99%의높은상동성을보였다. 균주 EDB-2는호기성, 그람양성, 막대형이며, 핑크빛을띠고있다. 생장가능온도는 20℃~45℃, 최적온도는약 20℃~42℃로생장온도범위가비교적넓은것으로나타났다. Casein과 cellulose 가수분해능력은없지만 DNA, agar, starch 그리고 tween의분해능력이있는것으로나타나지질분해능이우수하여유기물로오염된수산폐수를정화하는데유용하게쓰일수 있을것이다.
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公开(公告)号：KR101475589B1
公开(公告)日：2014-12-22
申请号：KR1020130008983
申请日：2013-01-27
Applicant: 전남대학교산학협력단
Abstract: 본발명에서는연안갯벌에서농화배양을통해엔도설판및 유기물분해능이매우우수한미생물 (균주명 EDB 2)를분리하여그 특성을분석하였다. EDB2 균주에대한생리생화학적및 유전학적특성을분석한결과와 99%의높은상동성을보였다. 균주 EDB-2는호기성, 그람양성, 막대형이며, 핑크빛을띠고있다. 생장가능온도는 20℃~45℃, 최적온도는약 20℃~42℃로생장온도범위가비교적넓은것으로나타났다. Casein과 cellulose 가수분해능력은없지만 DNA, agar, starch 그리고 tween의분해능력이있는것으로나타나지질분해능이우수하여지질로오염된환경정화에응용가능성높은것으로나타났다. Acetoin, urease, α-glucosidase, &bgr;-glucosidase, mannitol, malate, alkaline phosphate, esterase lipase, leucine arylamidase, trypsin, acid phosphate, esculin, sorbitol 양성을보였다.
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公开(公告)号：KR1020140094684A
公开(公告)日：2014-07-31
申请号：KR1020130006225
申请日：2013-01-20
Applicant: 전남대학교산학협력단
Abstract: The present invention separates a 11 strain which is included in a bacillus as a result of identifying based on a 16S rRNA sequence by separately selecting and purely culturing 31 colonies which are selected according to the color and shape of a colony which appears by growing a microorganism, which lives in a perinereis aibuhitensis which is known to have an excellent organism decomposition in a coastal mud flat, in an MA medium. The present invention can be used to purify a mud flat environment which is contaminated with organic matters since seven species of bacillus spp. including bacills algicola, bacills anthrasis, bacills aquimaris, bacills barbaricus, bacills hwajinpoensis, bacills nanhiensis, and bacills vietnamensis live in a perinereis aibuhitensis as a research result of analyzing the diversity of 11 strains which are included in a Bacillus genus and two species of strains which are selected by a separation from the perinereis aibuhitensis have an excellent function for decomposing a protein, a carbohydrate, and a lipid and have a tolerance to salt.
Abstract translation: 本发明通过分别选择并纯化培养根据通过生长微生物出现的菌落的颜色和形状选择的31个菌落，分离作为基于16S rRNA序列鉴定的结果的杆菌中包含的11菌株 ，其生活在围绕aibuhitensis，其已知在MA培养基中在沿海泥浆平板中具有优异的生物分解。 本发明可用于净化有机物质污染的泥浆环境，因为七种芽孢杆菌属 包括杆菌algicola，杆菌anthrasis，杆菌aquimaris，杆菌barbaricus，杆菌hwajinpoensis，杆菌nanhiensis和生活在perinereis aibuhitensis的杆菌，作为分析芽孢杆菌属和两种菌株中的11个菌株的多样性的研究结果 通过从围is中分离而选择的蛋白质具有分解蛋白质，碳水化合物和脂质并且具有对盐的耐受性的极好的功能。
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公开(公告)号：KR101447423B1
公开(公告)日：2014-10-06
申请号：KR1020120141224
申请日：2012-12-06
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 11 균주를 분리하였다.
Bacills 속(genus)에 포함되는 11 균주의 다양성을 분석한 결과
Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, Bacills vietnamensis 등 모두 7 종 (species)의
Bacillus spp.가 갯지렁이(
Perinereis aibuhitensis )에 내생하는 것으로 조사되었다. 본 발명에서 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주들은 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 갯벌 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.-
公开(公告)号：KR1020140096470A
公开(公告)日：2014-08-06
申请号：KR1020130008983
申请日：2013-01-27
Applicant: 전남대학교산학협력단
Abstract: The present invention isolates a microorganism with a bacterial name of EDB 2 in a coastal mud flat via enrichment and analyzes the characteristics of the microorganism that has excellent capacity for decomposing edosulfan and organic matters. As a result of the analysis of the physiobiochemical and genetic characteristics of the EDB2 bacterium, the EDB2 bacterium is 99% similar to Rhodococcus pyridinivorans. The EDB2 bacterium is aerobic, Gram-positive, bar-shaped and pink. The growth temperature range of the EDB2 bacterium is relatively wide as growability temperature ranges from 20 to 45°C and optimal temperature ranges from approximately 20 to 42°C. The EDB2 bacterium has strong possibility of being applied to purify lipid-polluted environment as the EDB2 bacterium does not have hydrolytic capacity for casein and cellulose but has decomposing capacity for DNA, agar, starch and tween, thus having excellent lipid-decomposing capacity. The EDB2 bacterium is acetoin-, urease-, α-glucosidase-, β-glucosidase-, mannitol-, malate-, alkalinephosphate-, esterase lipase-, leucine arylamidase-, trypsin-, acid phosphate-, esculin-, and sorbitol-positive.
Abstract translation: 本发明通过富集在沿海泥浆平板上分离细菌名称为EDB 2的微生物，并分析具有优异的分解能力的微生物的特性。 作为EDB2细菌的生理化学和遗传特征分析的结果，EDB2细菌与吡啶红霉素相似99％。 EDB2细菌是有氧的，革兰氏阳性，棒状和粉红色。 EDB2细菌的生长温度范围相对较宽，因为生长温度范围为20至45℃，最佳温度范围为约20至42℃。 由于EDB2细菌不具有酪蛋白和纤维素的水解能力，但对DNA，琼脂，淀粉和吐温具有分解能力，因此EDB2细菌具有很好的应用于净化脂质污染环境的可能性，具有优异的脂质分解能力。 EDB2细菌是乙偶姻，脲酶，α-葡萄糖苷酶，β-葡萄糖苷酶，甘露糖醇，苹果酸，磷酸 - 磷酸酯酶，酯酶脂肪酶，亮氨酸芳基酰胺酶，胰蛋白酶，酸式磷酸酯，esculin-和山梨糖醇 - 正。
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Patent Agency Ranking
公开(公告)号：KR1020130118588A
公开(公告)日：2013-10-30
申请号：KR1020120041564
申请日：2012-04-20
Applicant: 전남대학교산학협력단
CPC classification number: G01N1/10 , E02D1/04 , G01N2001/1025 , G01N2001/1031 , G01N2001/1418
Abstract: PURPOSE: A vacuum suction type of mud flat deposit collector has a sample collecting tube that can naturally enter inside a mud flat without applying artificial forces on a sample collection tube upon sample collection. CONSTITUTION: A vacuum suction type of mud flat deposit collector comprises the upper cap (30) and the cylinder rod (41). Said upper cap forms the cover (20) capable of opening and closing in the center of the top face. In said cylinder rod, a through-hole is formed in the center of said cover, around which the cylinder (40) and the first handle (10) are connected respectively to both end parts. A part of said upper cap is provided with the second handle (11). A lower part of said upper cap comprises the sample collection tube (50) that is divided into more than one and is connected.
Abstract translation: 目的：真空抽吸式泥浆沉积物收集器具有样品采集管，可以自然地进入泥浆平台，而不在样品收集管上对样品采集管施加人造力。 构成：真空抽吸式泥浆沉积物收集器包括上盖（30）和气缸杆（41）。 所述上盖形成能够在顶面的中心打开和关闭的盖（20）。 在所述气缸杆中，在所述盖的中心形成通孔，所述气缸（40）和所述第一把手（10）分别连接到两个端部。 所述上盖的一部分设置有第二手柄（11）。 所述上盖的下部包括分为多于一个并连接的样品收集管（50）。
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公开(公告)号：KR101477899B1
公开(公告)日：2015-01-08
申请号：KR1020130031960
申请日：2013-03-26
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 균주를 분리하였다. 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주 CBW4와 EBW4는 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 양식장 폐수 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.Abstract translation: 本发明涉及一种泻湖（泻湖）
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公开(公告)号：KR1020140117747A
公开(公告)日：2014-10-08
申请号：KR1020130032144
申请日：2013-03-26
Applicant: 전남대학교산학협력단
IPC: C02F3/34
Abstract: According to the present invention, microbes which live in Perinereis aibuhitensis in coastal mud flats have been grown in an MA culture medium and colonies have appeared, wherein the Perinereis aibuhitensis is known to have excellent degradability of organic matter. Thirty-one colonies have been separately selected according to the colors and shapes of the colonies which have appeared in the MA culture medium. Strains belonging to Bacillus genus have been isolated as a result of identification on the basis of 16S rRNA sequences after a pure culture process of the 31 colonies. Bacillus strains EBW4 and CBW4 isolated from the Perinereis aibuhitensis have excellent degradabilities of protein, carbohydrate and lipid and have resistance to salt. Accordingly, the EBW4 and CBW4 can be practically used to purify the wastewater environment of a farm contaminated with organic matter.
Abstract translation: 根据本发明，在沿海泥浆平面上生活在围Per中的微生物已经在MA培养基中生长，出现菌落，其中已知百日蓟马具有优异的有机物降解性。 根据出现在MA培养基中的菌落的颜色和形状分别选择了三十一个菌落。 在31个菌落的纯培养过程之后，基于16S rRNA序列的鉴定结果已经分离了属于芽孢杆菌属的菌株。 从百日蓟马分离的芽孢杆菌菌株EBW4和CBW4具有优异的蛋白质，碳水化合物和脂质的降解性，具有耐盐性。 因此，EBW4和CBW4实际上可用于净化有机物污染的农场的废水环境。
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公开(公告)号：KR1020140117732A
公开(公告)日：2014-10-08
申请号：KR1020130031984
申请日：2013-03-26
Applicant: 전남대학교산학협력단
CPC classification number: C02F3/341 , C02F2101/30 , C12N1/20
Abstract: The present invention separated a strain included in Bacillus sp. from the result of identification based on base sequence 16S rRNA after pure cultivating, by separately selecting thirty one colonies selected according to colors and shapes of colonies, generated by growing microorganism, arbuscular, in perinereis aibuhitensis known to have excellent ability of decomposing organic matters in mud flat along the shore, in MA medium. A strain CBW4 selected by being separated from perinereis aibuhitensis can be usefully used in purifying the environment filled with waste water in a farm contaminated due to the organic matters, by having salinity tolerance and excellent ability of decomposing protein, carbohydrate and lipid.
Abstract translation: 本发明分离芽孢杆菌属菌株中包含的菌株。 从纯培养后的基因序列16S rRNA的鉴定结果，通过分离选择根据生长的微生物生长的菌落的颜色和形状选择的三十一个菌落，已知具有优异的分解有机物质的能力的围is 沿岸平坦，MA中。 通过从围is中分离出来的菌株CBW4可以有效地用于净化由于有机物污染的农场中充满废水的环境，具有耐盐性和分解蛋白质，碳水化合物和脂质的优异能力。
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公开(公告)号：KR101414361B1
公开(公告)日：2014-07-01
申请号：KR1020120129282
申请日：2012-11-15
Applicant: 전남대학교산학협력단
Abstract: 본 발명은 연안 갯벌에서 유기물 분해능이 매우 우수한 것으로 알려진 갯지렁이(
Perinereis aibuhitensis )에 내생하고 있는 미생물을 MA 배지에서 생장하여 나타난 집락(colony)의 색깔과 모양에 따라 선별한 31개의 집락을 별도로 선별하여 순수배양한 후 16S rRNA 염기서열에 기초하여 동정한 결과,
Bacillus 속에 포함되는 11 균주를 분리하였다.
Bacills 속(genus)에 포함되는 11 균주의 다양성을 분석한 결과
Bacills algicola, Bacills anthrasis, Bacills aquimaris, Bacills barbaricus, Bacills hwajinpoensis, Bacills nanhiensis, Bacills vietnamensis 등 모두 7 종 (species)의
Bacillus spp.가 갯지렁이(
Perinereis aibuhitensis )에 내생하는 것으로 조사되었다. 본 발명에서 갯지렁이로부터 분리하여 선별한 두
Bacillus 균주들은 단백질, 탄수화물, 지질 분해능이 뛰어나고 염분에 내성을 갖고 있으므로 유기물로 오염된 갯벌 환경을 정화하는데 유용하게 쓰일 수 있을 것이다.Abstract translation: 本发明涉及一种泻湖（泻湖）
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