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1.发明授权
公开(公告)号：KR101549633B1
公开(公告)日：2015-09-03
申请号：KR1020130013612
申请日：2013-02-06
Applicant: 전남대학교산학협력단
Abstract: 본발명은감마아미노뷰티르산(GABA)을고수율로생산하는락토바실러스브레비스 K203균주및 이를이용한감마아미노뷰티르산생산방법에관한것으로그람염색법및 16S rDNA 염기서열분석을수행함으로써본 발명의K203균주를동정하였고, 얇은막 크로마토그래피와이온교환크로마토그래피를수행한후 배양액내 GABA를냉동건조하여정제함으로써고수율로 GABA를생산하는K203균주및 그특성을 GABA의대량생산에적용할수 있는뛰어난효과가있다.
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2.发明公开신규한 락토바실러스 브레비스 K203 균주 및 이를 이용한 감마 아미노뷰티르산의 고효율 생산방법 有权新颖的LACTOBACILLUS BREVIS K203和GAMMA AMINO BUTYRIC ACID（GABA）使用相同的高效生产
公开(公告)号：KR1020140100370A
公开(公告)日：2014-08-14
申请号：KR1020130013612
申请日：2013-02-06
Applicant: 전남대학교산학협력단
Abstract: The present invention relates to a Lactobacillus brevis K203 strain producing gamma-amino butyric acid (GABA) and a method using the same for producing GABA. The L.brevis K203 strain of the present invention is identified by analyzing a base sequence of 16S rDNA and using Gram staining method; and GABA in a culture medium is refined by freezing and drying after performing a thin film chromatography and an ion exchange chromatography. Therefore, the present invention has an advantage that the L.brevis K203 strain producing GABA in high yield and characteristics thereof are capable of being applied to mass production of GABA.
Abstract translation: 本发明涉及产生γ-氨基丁酸（GABA）的短乳杆菌K203菌株及其生产GABA的方法。 通过分析16S rDNA的碱基序列并使用革兰氏染色法鉴定本发明的L.brevis K203菌株; 通过在进行薄膜色谱法和离子交换色谱法后的冷冻干燥来提纯培养基中的GABA。 因此，本发明的优点在于，以高产率和特性产生GABA的L.brevis K203菌株能够适用于大量生产GABA。
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