公开(公告)号：KR1020100044465A

公开(公告)日：2010-04-30

申请号：KR1020080103610

申请日：2008-10-22

Applicant: 전북대학교산학협력단

Inventor： 강호영 ,  김삼웅 ,  이존화

IPC: C12N15/66 ,  C12N15/09

Abstract: PURPOSE: A recombinant vector for a development/secretion of a foreign protein is provided to improve the secretion efficiency and to confirm the development of the foreign protein by combining and epitope. CONSTITUTION: A recombinant vector for a development/secretion of a foreign protein contains a signal peptide, a protein development verifier epitope and an insertion site for the foreign protein. The signal peptide is either an OmpW signal peptide, a StfA signal peptide or a AgfA signal peptide. The protein development verifier epitope is either a His epitope, an RGS-His epitope, a T7 epitope or a V5 epitope. The insertion site for the foreign protein comprises an EcoRI, BamHI, SalI and HindIII cutting region. The producing method of the foreign protein comprises the following steps: culturing a transformant obtained by transforming a host cell using the recombinant vector; and confirming the foreign protein at a western blot.

Abstract translation: 目的：提供外源蛋白发育/分泌的重组载体，以提高分泌效率，并通过组合和表位证实外源蛋白的发育。 构成：用于外源蛋白质的发育/分泌的重组载体含有信号肽，蛋白质发育验证子表位和外来蛋白质的插入位点。 信号肽是OmpW信号肽，StfA信号肽或AgfA信号肽。 蛋白质发育验证者表位是His表位，RGS-His表位，T7表位或V5表位。 外源蛋白质的插入位点包含EcoRI，BamHI，SalI和HindIII切割区。 外源蛋白的制造方法包括以下步骤：培养使用重组载体转化宿主细胞获得的转化体; 并在western印迹中确认外源蛋白。

公开(公告)号：KR1020100044466A

公开(公告)日：2010-04-30

申请号：KR1020080103611

申请日：2008-10-22

Applicant: 전북대학교산학협력단

Inventor： 강호영 ,  김삼웅 ,  이존화

IPC: A61K39/112 ,  A61P37/06

CPC classification number: A61K39/0275 ,  A61K2039/52 ,  Y10S514/885

Abstract: PURPOSE: A live vaccine composition containing bacteria incapable of surviving at the environment is provided to easily produce and store the vaccine, and to safely and effectively prevent diseases when injecting to domestic animals. CONSTITUTION: A live vaccine composition containing auxotrophic bacteria which is lack of essential genes necessary for the outgrowth of a cell, and need to be supplied with the essential genes from a auxotrophy recombinant vector. The auxotrophic bacteria are either the Salmonella typhi, the S. typhimurium, the S. enteritidis, the S. galinarum, the S. dublin, the S. Cholerae-suis or the S. pullorum. The essential genes necessary for the outgrowth of the cell is aspartate kinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate reductase, glutamate:succinyl-diaminopimelate aminotransferase, or succinyldiaminopimelate desuccinylase.

Abstract translation: 目的：提供一种含有不能在环境中存活的细菌的活疫苗组合物，用于容易地生产和储存疫苗，并在注射到家畜时安全有效地预防疾病。 构成：含有营养缺陷型细菌的活疫苗组合物，其缺乏细胞生长所需的基本基因，需要从营养缺陷型重组载体中提供必需基因。 营养缺陷型细菌是伤寒沙门氏菌，鼠伤寒沙门氏菌，肠炎沙门氏菌，加勒红细菌，都柏林，霍乱弧菌或沙门氏菌。 细胞生长必需的必需基因是天冬氨酸激酶，天冬氨酸半醛脱氢酶，二氢吡啶二羧酸合成酶，二氢吡啶二羧酸还原酶，四氢吡啶二羧酸还原酶，谷氨酸：琥珀酰 - 二氨基庚酸酯氨基转移酶或琥珀酰二氨基庚二酸脱琥珀酰酶。

公开(公告)号：KR101044332B1

公开(公告)日：2011-06-29

申请号：KR1020080103610

申请日：2008-10-22

Applicant: 전북대학교산학협력단

Inventor： 강호영 ,  김삼웅 ,  이존화

IPC: C12N15/66 ,  C12N15/09

Abstract: 본 발명은 외래 단백질의 발현ㆍ분비용 재조합 벡터에 관한 것으로, 더욱 상세하게는 시그널 펩티드, 단백질 발현 확인용 표지(epitope), 외래 유전자 삽입 부위를 함유하는 외래 단백질의 발현ㆍ분비용 재조합 벡터, 이를 숙주세포에 도입하여 수득되는 형질전환 미생물 및 상기 형질전환 미생물을 이용한 외래 단백질의 분비생산방법에 관한 것이다.
본 발명에 따르면, 기존의 시그널 펩티드로 분비되는 시스템에 비하여 외래 단백질의 분비 효율을 높일 수 있고, 단백질 발현 확인용 표지(epitope)를 결합하여 편리하게 외래 단백질의 발현을 확인할 수 있다.
β-lactamase(bla), agfA, ompW, stfA, signal peptide, His-epitope

公开(公告)号：KR100873168B1

公开(公告)日：2008-12-10

申请号：KR1020070022620

申请日：2007-03-07

Applicant: 전북대학교산학협력단

Inventor： 강호영 ,  김삼웅 ,  이존화 ,  문기환

IPC: C12N1/21 ,  C12N15/03 ,  C12N15/09

Abstract: 본 발명은
lon 유전자 및/또는
cpxR 유전자가 결실된 살모넬라 변이균주, 그 제조방법 및 상기 변이균주를 함유하는 약독화된 살모넬라 백신조성물에 관한 것이다.
본 발명에 따른 약독화된 살모넬라 생백신은 생산 및 보관이 용이하여 경제적이며, 가축에 투여 시, 안전하고 효율적으로 살모넬라 감염을 예방할 수 있다.
살모넬라, 생백신, 약독화, 변이균주

公开(公告)号：KR1020080082156A

公开(公告)日：2008-09-11

申请号：KR1020070022620

申请日：2007-03-07

Applicant: 전북대학교산학협력단

Inventor： 강호영 ,  김삼웅 ,  이존화 ,  문기환

IPC: C12N1/21 ,  C12N15/03 ,  C12N15/09

Abstract: An attenuated Salmonella live vaccine is provided to be easily and economically produced and stored and prevent Salmonella infection safely and efficiently when administered to a domestic animal. A method for preparing a Salmonella live vaccine comprises a step of deleting a lon or a cpxR gene from a chromosome DNA of a Salmonella strain such as Salmonella typhimurium, S. typhi, S. paratyphi, S. sendai, S. gallinarium and S. enteritidis. A vector includes a DNA where a DNA fragment linked to a 5'-terminal of the lon gene derived from the Salmonella strain and a DNA fragment linked to a 3'-terminal of the lon gene are bonded. An attenuated Salmonella vaccine composition comprises a Salmonella mutant where the lon gene and the cpxR gene are deleted from the chromosome DNA of the Salmonella and a pharmaceutically acceptable carrier.

Abstract translation: 提供减毒的沙门氏菌活疫苗，以便容易经济地生产和储存，并且当施用于家畜时安全有效地预防沙门氏菌感染。 一种制备沙门氏菌活疫苗的方法，包括从沙门氏菌菌株如鼠伤寒沙门氏菌，伤寒沙门氏菌，副伤寒沙门氏菌，沙门氏菌，沙门氏菌和沙门氏菌等染色体DNA中删除lon或cpxR基因的步骤。 肠炎。 载体包括与来自沙门氏菌属菌株的lon基因的5'末端连接的DNA片段和与lon基因的3'端连接的DNA片段连接的DNA。 减毒沙门氏菌疫苗组合物包含沙门氏菌突变体，其中lon基因和cpxR基因从沙门氏菌的染色体DNA和药学上可接受的载体中缺失。