公开(公告)号：KR100814504B1

公开(公告)日：2008-03-18

申请号：KR1020060108483

申请日：2006-11-03

Applicant: 전북대학교산학협력단

Inventor： 강길선 ,  박기숙 ,  김수미 ,  김초민

IPC: C12N5/0793

CPC classification number: C12N5/0619 ,  C12N2501/115 ,  C12N2501/195 ,  C12N2506/1353

Abstract: A method for differentiating mesenchymal stem cells into neurons is provided to induce the differentiation of the mesenchymal stem cells into the neurons effectively using a poly lactic acid-co-glycolic acid(PLGA) film and show excellent efficiency compared with the differentiation into the neurons using a conventional culture solution. A method for differentiating mesenchymal stem cells into neurons comprises a step of differentiating the mesenchymal stem cells on a poly lactic acid-co-glycolic acid(PLGA) film including neurogenic differentiation inducing materials such as butylated hydroxy anisole(BHA), beta-mercaptoethanol and dimethylsulfoxide(DMSO), wherein the mesenchymal stem cells are pre-treated with a basic-fibroblast growth factor for 12-24 hours.

Abstract translation: 提供了将间充质干细胞分化为神经元的方法，以使用聚乳酸 - 共 - 乙醇酸（PLGA）膜有效地诱导间充质干细胞分化为神经元，与使用 常规培养方案。 用于将间充质干细胞分化为神经元的方法包括将间充质干细胞分离在聚乳酸 - 共 - 乙醇酸（PLGA）膜上的步骤，所述聚乳酸 - 共 - 乙醇酸（PLGA）膜包括神经源性分化诱导材料，例如丁基化羟基苯甲醚（BHA），β-巯基乙醇和 二甲基亚砜（DMSO），其中间充质干细胞用碱性成纤维细胞生长因子预处理12-24小时。