公开(公告)号：KR100744833B1

公开(公告)日：2007-08-01

申请号：KR1020060018402

申请日：2006-02-24

Applicant: 전북대학교산학협력단

Inventor： 강길선 ,  박기숙 ,  김순희 ,  고종태 ,  최방실 ,  하현정 ,  고연경 ,  윤선중

IPC: A61F2/44 ,  A61F2/30 ,  A61F2/28 ,  A61F2/02

Abstract: An artificial intervertebral disc comprising a disc cell and a method of regenerating a tissue engineered disc using the same disc cell are provided to regenerate a disc using an artificial disc comprising a scaffold having the inside and outside comprising different component. An artificial intervertebral disc comprising a disc cell includes: an inside disc including a disc central shaft in a disc shape and comprising biodegradable porous scaffold including nucleus pulposus cells; and an outside disc surrounding the inside disc in a doughnut shape, and comprising biodegradable porous scaffold including annulus fibrous cells. The artificial intervertebral disc further includes a natural or synthetic biomaterial added to the biodegradable porous scaffold of the inside or the outside of the disc.

Abstract translation: 提供一种包括椎间盘细胞的人造椎间盘和使用相同椎间盘细胞再生组织工程椎间盘的方法，以使用包括内部和外部包含不同组分的支架的人造椎间盘再生椎间盘。 包括椎间盘细胞的人造椎间盘包括：内椎间盘，所述内椎间盘包括椎间盘中心轴并且包括包括髓核细胞的生物可降解多孔支架; 以及环形内圆盘的外圆盘，其为圆环形，并且包括包含环形纤维细胞的可生物降解的多孔支架。 人造椎间盘还包括添加到盘内部或外部的生物可降解多孔支架的天然或合成生物材料。

公开(公告)号：KR100814504B1

公开(公告)日：2008-03-18

申请号：KR1020060108483

申请日：2006-11-03

Applicant: 전북대학교산학협력단

Inventor： 강길선 ,  박기숙 ,  김수미 ,  김초민

IPC: C12N5/0793

CPC classification number: C12N5/0619 ,  C12N2501/115 ,  C12N2501/195 ,  C12N2506/1353

Abstract: A method for differentiating mesenchymal stem cells into neurons is provided to induce the differentiation of the mesenchymal stem cells into the neurons effectively using a poly lactic acid-co-glycolic acid(PLGA) film and show excellent efficiency compared with the differentiation into the neurons using a conventional culture solution. A method for differentiating mesenchymal stem cells into neurons comprises a step of differentiating the mesenchymal stem cells on a poly lactic acid-co-glycolic acid(PLGA) film including neurogenic differentiation inducing materials such as butylated hydroxy anisole(BHA), beta-mercaptoethanol and dimethylsulfoxide(DMSO), wherein the mesenchymal stem cells are pre-treated with a basic-fibroblast growth factor for 12-24 hours.

Abstract translation: 提供了将间充质干细胞分化为神经元的方法，以使用聚乳酸 - 共 - 乙醇酸（PLGA）膜有效地诱导间充质干细胞分化为神经元，与使用 常规培养方案。 用于将间充质干细胞分化为神经元的方法包括将间充质干细胞分离在聚乳酸 - 共 - 乙醇酸（PLGA）膜上的步骤，所述聚乳酸 - 共 - 乙醇酸（PLGA）膜包括神经源性分化诱导材料，例如丁基化羟基苯甲醚（BHA），β-巯基乙醇和 二甲基亚砜（DMSO），其中间充质干细胞用碱性成纤维细胞生长因子预处理12-24小时。