Abstract:
The present invention relates to a method for high production of thermostable recombinant arabinanase enzyme originated from phanerochaete chrysosporium. If arabinanase and xylanase produced from transformed yeast of the present invention are processed in biomass, the efficiency of biomass decomposition is increased by synergism of the two enzymes. Arabinanase of the present invention has high value in industrial utilization as a coenzyme added to feed additives, additives for paper manufacture, detergent additives, additives for biomass preprocess to produce bio-energy, etc.
Abstract:
본 발명의 백색부후균 유래의 엔도-1,4-베타 자일라나제 C 대량생산을 위한 재조합 발현벡터 및 이를 이용한 엔도-1,4-베타 자일라나제 C 대량생산방법은 종래의 재조합 발현벡터 및 이를 이용한 대량생산방법에 비하여 현저하게 많은 양의 엔도-1,4-베타 자일라나제 C를 대량으로 생산할 수 있다.
Abstract:
본 발명의 백색부후균 유래의 엔도-1,4-베타 자일라나제 A 대량생산을 위한 재조합 발현벡터 및 이를 이용한 엔도-1,4-베타 자일라나제 A 대량생산방법은 종래의 재조합 발현벡터 및 이를 이용한 대량생산방법에 비하여 현저하게 많은 양의 엔도-1,4-베타 자일라나제 A를 대량으로 생산할 수 있다.
Abstract:
PURPOSE: A gene encoding manganese peroxidase derived from Phanerochaete chrysosporium BKM-F-1767 and an expression method thereof are provided to produce bioethanol. CONSTITUTION: A gene encoding manganese peroxidase(MnP) has a base sequence of sequence number 1, derived from Phanerochaete chrysosporium BKM-F-1767 and an amino acid of sequence number 2. An expression method of a gene encoding MnP comprises: a step of inserting a gene encoding MnP into a pPICZαA expression vector and preparing a recombinant expression vector; a step of transducing the recombinant expression vector into Pichia pastoris GS115 and transforming with an alcoholoxide I(AOX 1) promoter in the vector for extracellular expression. An enzyme agent for decomposing ligneous biomass contains expressed MnP as an active ingredient.
Abstract:
PURPOSE: A recombinant expression vector and a method for preparing endo-1,4-beta xylanase-C are provided to obtain a large amount of endo-1,4-beta xylanase-C. CONSTITUTION: A recombinant expression vector for preparing a large amount of endo-1,4-beta xylanase-C is operatively linked with a nucleic acid sequence of sequence number 2 encoding endo-1,4-beta xylanase-C without a nucleic acid sequence encoding AOXI promoter which is regulated by methanol, a nucleic acid sequence encoding alpha-secretion factor derived from yeast, and a secretion signal sequence derived from Phanerochaete chrysosporium. The Phanerochaete chrysosporium is Phaenerocheate chrysosporium BKM-F-1767(KCTC 6147). A method for preparing endo-1,4-beta xylanase-C comprises: a step of preparing the recombinant expression vector; a step of transforming the recombinant expression vector to yeast; and a step of culturing the transformed yeast in a culture machine containing methanol and isolating and purifying endo-1,4-beta xylanase-C.
Abstract:
The present invention relates to a method for highly producing a multi-functional recombinant xylosidase/arabinofuranosidase enzyme which is derived from a phanerochaete chrysosporium. The present invention is able to largely produce a recombinant β-xylosidase/α-L-arabinofuranosidase which is a decomposition enzyme of hemicellulose which can be widely used in feed additives, paper manufacturing, detergent, and bio energy production by using a yeast (Pichia pastoris) transformed from a β-xylosidase/α-L- arabinofuranosidase gene which is derived from a Phanerochate chrysosporium.
Abstract:
PURPOSE: A method for producing endo-1,4-beta xylanase C using a recombinant expression vector is provided to obtain a large amount of endo-1,4-beta xylanase C. CONSTITUTION: A recombinant expression vector for producing a large amoount of endo-1,4-beta xylanase C contains: a nucleic acid sequence encoding an AOXI promoter regulated by methanol; and a nucleic acid encoding endo-1,4-beta-xylanase C containing white rot fungi- secretion signal sequences, which is operatively linked in a transcription direction. The white rot fungi is Phaenerocheate chrysosporium BKM-F-1767(KCTC 6147).
Abstract:
본 발명은 백색 부후균 유래의 탄수화물 결합 모듈을 갖는 재조합 아세틸 자일란 에스터라아제 다기능 효소의 고생산 방법에 관한 것으로, 백색 부후균 유래의 탄수화물 결합 모듈 (CBM)을 갖는 아세틸 자일란 에스터라아제 유전자로 형질전환된 효모에서 생산된 아세틸 자일란 에스터라아제는 자일란 구조 (backbone)의 아세틸기를 제거하고 자일란 및 리그닌을 분해하는 활성을 가질 뿐만 아니라, 자일라나아제와 동시에 사용했을 때 바이오매스 분해 활성을 증가시킬 수 있으므로 사료 첨가제, 종이 제조, 세제, 리그노셀룰로오스계 바이오매스 분해 및 바이오 에너지 생산 등을 위한 산업적 효소로 유용하게 이용할 수 있을 것으로 기대된다.