公开(公告)号：KR1020170053223A

公开(公告)日：2017-05-16

申请号：KR1020150155425

申请日：2015-11-06

Applicant: 전북대학교산학협력단

Inventor： 양문식 ,  김태금 ,  장용석 ,  김대혁 ,  김미영

IPC: C12N15/82 ,  A61K39/12

CPC classification number: Y02A50/386

Abstract: 본발명은식물바이러스발현시스템을이용한재조합뎅기바이러스백신을대량생산하는방법에관한것으로, 바이러스유래 RNA 중합효소를코딩하는유전자를포함하는 5' 프로벡터,또는유전자를포함하는 3' 프로벡터및 부위특이적리콤비나제(recombinase)를코딩하는유전자를포함하는제3의벡터로동시에형질전환시킨아그로박테리움을식물체에침윤하여식물체내에서 cEDⅢ또는 cEDIII-Co1 단백질을대량으로생산할수 있음을확인하였다. 본발명의식물바이러스발현시스템을이용하여재조합뎅기바이러스백신을대량생산할수 있으므로, 저생산비용으로, 안전하게뎅기바이러스에대한면역효과를갖는백신을대규모로생산하는데매우유용하다.

Abstract translation: 本发明涉及一种使用植物病毒表达系统批量生产重组登革病毒疫苗的方法，更具体地涉及一种使用5'端大规模生产重组登革病毒疫苗的方法， 它是由渗透的特定组合痢疾证实木聚糖酶（重组酶）农杆菌与第三载体同时转化，包括编码日光浴，可以产生在植物体上的大量的cEDⅢ或cEDIII-CO1蛋白的植物的基因。 因为通过使用本发明的植物病毒表达系统也可以大量生产重组登革病毒疫苗，以低的生产成本，并且是用于安全地生产具有大范围上登革热病毒的免疫效果的疫苗是非常有用的。

公开(公告)号：KR1020140090501A

公开(公告)日：2014-07-17

申请号：KR1020130002646

申请日：2013-01-09

Applicant: 전북대학교산학협력단

Inventor： 김대혁 ,  양문식 ,  장용석

IPC: C12N15/63 ,  C12N15/81 ,  C12N1/19 ,  A61K39/12

CPC classification number: Y02A50/386

Abstract: The present invention relates to a method for producing a recombinant dengue virus vaccine by using yeast, and to a dengue virus vaccine followed thereby. Recombinant scEDIII protein produced from yeast is capable of inducing an antibody to all dengue viruses of four serotypes, so a stable and safe recombinant dengue virus vaccine having cross-neutralizing activity can be provided. The present invention has an effect of easily purifying the antibody since the scEDIII protein produced by a rice α-amylase signal peptide sequence is secreted inside a medium.

Abstract translation: 本发明涉及使用酵母制造重组登革热病毒疫苗的方法及其后的登革热病毒疫苗。 从酵母产生的重组scEDIII蛋白能够诱导四种血清型的所有登革热病毒的抗体，因此可提供具有交叉中和活性的稳定和安全的重组登革热病毒疫苗。 本发明具有容易地纯化抗体的效果，因为由水稻α-淀粉酶信号肽序列产生的scEDIII蛋白质在培养基内分泌。

公开(公告)号：KR101418784B1

公开(公告)日：2014-07-11

申请号：KR1020130012708

申请日：2013-02-05

Applicant: 전북대학교산학협력단 ,  (주)엔비엠

Inventor： 박승문 ,  김대혁 ,  권태호

IPC: C12N9/14 ,  C12N15/62 ,  C12N15/63 ,  C12N15/81

Abstract: The present invention relates to a method for highly producing a multi-functional recombinant xylosidase/arabinofuranosidase enzyme which is derived from a phanerochaete chrysosporium. The present invention is able to largely produce a recombinant β-xylosidase/α-L-arabinofuranosidase which is a decomposition enzyme of hemicellulose which can be widely used in feed additives, paper manufacturing, detergent, and bio energy production by using a yeast (Pichia pastoris) transformed from a β-xylosidase/α-L- arabinofuranosidase gene which is derived from a Phanerochate chrysosporium.

Abstract translation: 本发明涉及一种高度生产多功能重组木糖苷酶/阿拉伯呋喃糖苷酶的方法，所述多功能重组木糖苷酶/阿拉伯呋喃糖苷酶衍生自金孢子虫（Phanerochaete chrysosporium）。 本发明能够在很大程度上产生作为半纤维素分解酶的重组β-木糖苷酶/α-L-阿拉伯呋喃糖苷酶，其可以广泛用于饲料添加剂，造纸，洗涤剂和使用酵母的生物能量生产（毕赤氏酵母 巴斯德毕赤氏酵母）从衍生自Phanerochate chrysosporium的β-木糖苷酶/α-L-阿拉伯呋喃糖苷酶基因转化。

公开(公告)号：KR1020120093573A

公开(公告)日：2012-08-23

申请号：KR1020110013223

申请日：2011-02-15

Applicant: 전북대학교산학협력단

Inventor： 박승문 ,  김대혁

IPC: C12N15/63 ,  C12N15/56 ,  C12N1/19 ,  C12N9/24

Abstract: PURPOSE: A method for producing endo-1,4-beta xylanase C using a recombinant expression vector is provided to obtain a large amount of endo-1,4-beta xylanase C. CONSTITUTION: A recombinant expression vector for producing a large amoount of endo-1,4-beta xylanase C contains: a nucleic acid sequence encoding an AOXI promoter regulated by methanol; and a nucleic acid encoding endo-1,4-beta-xylanase C containing white rot fungi- secretion signal sequences, which is operatively linked in a transcription direction. The white rot fungi is Phaenerocheate chrysosporium BKM-F-1767(KCTC 6147).

Abstract translation: 目的：提供使用重组表达载体产生内切-1,4-β木聚糖酶C的方法，以获得大量内切-1,4-β木聚糖酶C.构成：用于产生大量 内切-1,4-β木聚糖酶C包含：编码由甲醇调节的AOXI启动子的核酸序列; 以及编码含有白腐真菌分泌信号序列的内-1,4-β-木聚糖酶C的核酸，其在转录方向上可操作地连接。 白腐菌是Phaenerocheate chrysosporium BKM-F-1767（KCTC 6147）。

公开(公告)号：KR100567733B1

公开(公告)日：2006-04-04

申请号：KR1020050055565

申请日：2005-06-27

Applicant: 전북대학교산학협력단

Inventor： 김대혁 ,  양문식 ,  장용석

IPC: C12N15/81

Abstract: 본 발명은 이종중합형 패리틴을 포함하는 식품 첨가제에 관한 것이다. 특히 본 발명은 생체이용성이 우수한 형태의 철을 다량 저장할 수 패리틴을 포함하는 식품 첨가제에 관한 것으로, (a) 단일 숙주세포에 패리틴 라이트체인이 패리틴 헤비체인에 비하여 높은 분자비율로 발현되도록 고안한 패리틴 라이트체인 발현용 벡터 I 및 패리틴 헤비체인 발현용 벡터 II를 순차적으로 도입하고, (b) 상기 두 벡터가 모두 도입된 형질전환체를 선별하고 및 (c) 상기 형질전환체를 배양하여 이종중합형 패리틴을 발현시키는 단계를 포함하는 방법으로 제조되는 이종중합형 패리틴을 포함하는 식품 첨가제를 제공한다.
패리틴, 철, 이종중합형, 식품 첨가제.

公开(公告)号：KR1020030086062A

公开(公告)日：2003-11-07

申请号：KR1020020024424

申请日：2002-05-03

Applicant: 전북대학교산학협력단

Inventor： 장용석 ,  양문식 ,  김대혁

IPC: C12N15/83

CPC classification number: C12N15/8202 ,  A61K39/215 ,  A61K2039/542 ,  A61K2039/552 ,  C07K14/005 ,  C12N15/8258 ,  C12N2770/20034

Abstract: PURPOSE: Transformants expressing the epitope protein of porcine epidemic diarrhea virus and an oral vaccine composition containing the same are provided. The transformants can be easily stored, maintained, transported and administered to animals. CONSTITUTION: A recombinant vector is characterized by containing a PEDV epitope gene encoding PEDV epitope protein having the amino acid sequence of SEQ ID NO: 1, wherein X22 is Asp or Gly, X23 is Ser or Leu, X24 is Ser or Gly, X25 is Ser or Gly, X51 is Thr or Arg, X58 is Asn or Ser, X114 is Leu or Phe, and X137 is IIe or Val; and the PEDV epitope gene has the nucleotide sequence of SEQ ID NO: 2; the vector contains a plant expression vector, the PEDV epitope gene and a transcription terminator; the recombinant vector is pMY039(KCTC 10194BP). Transformants are produced with the recombinant vector, wherein the transformant is Agrobacterium, Escherichia coli or transgenic plants; the plant is selected from tobacco, Arabidopsis thaliana, potato, lettuce, radish, Chinese cabbage, carrot, tomato, bean, rice, barley, wheat and corn.

Abstract translation: 目的：提供表达猪流行性腹泻病毒表位蛋白质的转化体和含有其的口服疫苗组合物。 转化体可以容易地储存，维持，运输和给予动物。 构成：重组载体的特征在于含有编码具有SEQ ID NO：1的氨基酸序列的PEDV表位蛋白的PEDV表位基因，其中X22为Asp或Gly，X23为Ser或Leu，X24为Ser或Gly，X25为 Ser或Gly，X51为Thr或Arg，X58为Asn或Ser，X114为Leu或Phe，X137为IIe或Val; 并且所述PEDV表位基因具有SEQ ID NO：2的核苷酸序列; 载体含有植物表达载体，PEDV表位基因和转录终止子; 重组载体是pMY039（KCTC 10194BP）。 用重组载体产生转化体，其中转化体是农杆菌，大肠杆菌或转基因植物; 该植物选自烟草，拟南芥，马铃薯，莴苣，萝卜，大白菜，胡萝卜，番茄，豆，大米，大麦，小麦和玉米。

公开(公告)号：KR101500697B1

公开(公告)日：2015-03-10

申请号：KR1020130018689

申请日：2013-02-21

Applicant: 전북대학교산학협력단

Inventor： 김대혁 ,  양문식 ,  김정미

IPC: C12Q1/00 ,  C12Q1/24 ,  G01N33/569

Abstract: 본 발명은 마이코바이러스에 감염된 진균류의 마이코바이러스 제거 방법에 관한 것으로, 본 발명의 방법을 이용하면 마이코바이러스 (mycovirus)에 감염된 진균류에서 마이코바이러스를 간단하고 효율적으로 제거할 수 있다. 따라서, 바이러스가 없는 (vius-free) 균주를 대량으로 확보할 수 있으므로 버섯 종균 생산에 유용할 것으로 기대된다.

公开(公告)号：KR1020140100715A

公开(公告)日：2014-08-18

申请号：KR1020130013785

申请日：2013-02-07

Applicant: 전북대학교산학협력단 ,  (주)엔비엠

Inventor： 박승문 ,  김대혁 ,  권태호

IPC: C12N9/16 ,  C12N15/55 ,  C12N15/63 ,  C07K16/40

Abstract: The present invention relates to a method for high production of recombinant bifunctional acetyl xylan esterase with a carbohydrate binding module derived from phanerochaete chrysosporium. Acetyl xylan esterase produced from yeast which is transformed to an acetyl xylan esterase gene with a carbohydrate binding module(CBM) derived from phanerochaete chrysosporium not only has an activity of removing an acetyl group of a xylan backbone structure and decomposing xylan and lignin, but also improves an activity of decomposing biomass when used with xylanase at the same time, thereby being used as an industrial enzyme for feed additives, production of paper, a detergent, decomposition of lingo-cellulose-based biomass, and production of bio-energy.

Abstract translation: 本发明涉及一种高产量生产重组双功能乙酰木聚糖酯酶的方法，该方法具有衍生自phanerochaete chrysosporium的碳水化合物结合模块。 由酵母生产的由乙酰木聚糖酯酶基因转化成乙酰木聚糖酯酶基因的乙酰木聚糖酯酶不仅具有去除木聚糖骨架结构的乙酰基并分解木聚糖和木质素的活性， 提高了与木聚糖酶同时使用时分解生物质的活性，从而被用作饲料添加剂的工业酶，纸，洗涤剂的生产，基于纤维素的生物质的分解和生物能的生产。

公开(公告)号：KR1020130002311A

公开(公告)日：2013-01-07

申请号：KR1020120155351

申请日：2012-12-27

Applicant: 전북대학교산학협력단

Inventor： 박승문 ,  김대혁

IPC: C12N15/63 ,  C12N15/56 ,  C12N15/81 ,  C12N9/24

Abstract: PURPOSE: A recombinant expression vector for producing a large amount of endo-1,4-xylanase-A derived from Phanerochaete chrysosporium is provided to bleach pulp paper and to decompose hemicellulose. CONSTITUTION: A recombinant expression vector for producing a large amount of endo-1,4-beta xylanase-A is linked with a nucleic acid of sequence number 2 which encodes endo-1,4-beta xylanase-A without a nucleic acid sequence encoding AOXI promoter which is controlled by methanol, a nucleic acid sequence encoding an alpha-secretion factor derived from yeast, and a secretion signal sequence derived from Phanerochaete chrysosporium. The Phanerochaete chrysosporium is Phaenerocheate chrysosporium BKM-F-1767(KCTC 6147). The yeast which produces endo-1,4-beta xylanase-A is prepared by transforming with the recombinant expression vector. The yeast is Pichia pastoris.

Abstract translation: 目的：提供从Phanerochaete chrysosporium产生大量内切-1,4-木聚糖酶-A的重组表达载体，用于漂白纸浆和分解半纤维素。 构成：用于产生大量内切-1,4-β木聚糖酶-A的重组表达载体与编码内切-1,4-β木聚糖酶-A的序列号2的核酸连接，而不具有编码的核酸序列 由甲醇控制的AOXI启动子，编码来自酵母的α-分泌因子的核酸序列和源自Phanerochaete chrysosporium的分泌信号序列。 Phanerochaete chrysosporium是Phaenerocheate chrysosporium BKM-F-1767（KCTC 6147）。 通过用重组表达载体转化制备产生内切-1,4-β木聚糖酶-A的酵母。 酵母是巴斯德毕赤酵母。

公开(公告)号：KR1020120093571A

公开(公告)日：2012-08-23

申请号：KR1020110013220

申请日：2011-02-15

Applicant: 전북대학교산학협력단

Inventor： 박승문 ,  김대혁

IPC: C12N15/63 ,  C12N15/56 ,  C12N1/19 ,  C12N9/42

Abstract: PURPOSE: A method for producing a large amount of endo-1,4-beta xylanase A using a recombinant vector is provided to isolate and purify the enzyme by affinity chromatography. CONSTITUTION: A recombinant expression vector for producing a large amount of endo-1,4-beta xylanase A comprises: a nucleic acid sequence encoding an AOXI promoter regulated by methanol; and a nucleic acid sequence encoding endo-1,4-beta xylanase A containing secretion signal sequence derived from white rot fungi. The white rot fungi are Phaenerocheate chrysosporium BKM-F-1767(KCTC 6147). The endo-1,4-beta xylanase A containing white rot fungi-derived secretion signal sequences is a nucleic acid sequence of sequence number 1.

Abstract translation: 目的：提供使用重组载体产生大量内切-1,4-β木聚糖酶A的方法，通过亲和层析分离和纯化酶。 构成：用于产生大量内切-1,4-β木聚糖酶A的重组表达载体包括：编码由甲醇调节的AOXI启动子的核酸序列; 以及编码含有来自白腐真菌的分泌信号序列的内-1,4-β-木聚糖酶A的核酸序列。 白腐菌是Phaenerocheate chrysosporium BKM-F-1767（KCTC 6147）。 含有白腐真菌来源的分泌信号序列的内切-1,4-β木聚糖酶A是序列号1的核酸序列。