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公开(公告)号：KR1020110023427A
公开(公告)日：2011-03-08
申请号：KR1020090081317
申请日：2009-08-31
Applicant: 전북대학교산학협력단
Abstract: PURPOSE: A solid culture composition for passage culturing inonotus obliquus mycelia is provided to offer the optimum cultivation condition for the inonotus obliquus mycelia for mass producing protein-bound polysaccharide. CONSTITUTION: A solid culture composition for passage culturing inonotus obliquus mycelia contains 16~32g/L of glucose, 8~16g/L of brown rice, 8~16g/L of peptone, and 3~8g/L of magnesium sulfate. The solid culture composition is obtained by additionally adding distilled water, and sterilizing the mixture.
Abstract translation: 目的：提供一种固体培养组合物，用于通过培养阳离子斜方菌丝体，为大量生产蛋白质结合多糖的马蹄莲菌丝体提供最佳的培养条件。 构成：含有16〜32g / L葡萄糖，8〜16g / L的糙米，8〜16g / L的胨和3〜8g / L的硫酸镁的固体培养物。 固体培养组合物通过另外加入蒸馏水并对混合物进行灭菌而获得。
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公开(公告)号：KR101100292B1
公开(公告)日：2011-12-28
申请号：KR1020090074871
申请日：2009-08-13
Applicant: 전북대학교산학협력단
Abstract: 본 발명은 단백다당체 생산능을 가지는 신균주 차가버섯(
Inonotus obliquus ) M5에 관한 것으로, 보다 상세하게는 차가버섯(
Inonotus obliquus ) 균사체를 UV처리한 후 원형질체를 형성시키고 상기 원형질체의 재생과정으로 제조된 신균주 차가버섯(
Inonotus obliquus ) M5 및 상기의 신균주를 배양하고 그 배양액으로부터 단백다당체를 생산하는 단계를 포함하는 것을 특징으로 하는 단백다당체의 제조방법에 관한 것이다.
본 발명의 신균주 차가버섯(
Inonotus obliquus ) M5는 친균주인 차가버섯 균주에 비해 균체성장률 및 단백다당체의 생산성이 높으며, 따라서 본 발명에 의해 면역증강, 항암, 항당뇨 효능 등을 나타내는 차가버섯 유래 단백다당체의 대량공급이 가능하다.
차가버섯M5, 단백다당체, 차가버섯 균사체, 원형질체, 재생-
公开(公告)号：KR1020110023489A
公开(公告)日：2011-03-08
申请号：KR1020090081409
申请日：2009-08-31
Applicant: 전북대학교산학협력단
Abstract: PURPOSE: A liquid culture medium composition for culturing inonotus obliquus mycelia is provided to mass-produce protein-bound polysaccharide having an immunity enhancement effect. CONSTITUTION: A liquid culture medium composition for culturing inonotus obliquus mycelia contains 8~16g/L of glucose, 20~32g/L of fructose, and 4~8g/L of soytone peptone. The culturing method of the inonotus obliquus mycelia on the liquid culture medium composition comprises the following steps: passage culturing the inonotus obliquus mycelia on an agar medium; static culturing the inonotus obliquus mycelia on a stationary culture medium; and liquid culturing the inonotus obliquus mycelia on a liquid medium.
Abstract translation: 目的：提供一种用于培养内耳斜管菌丝体的液体培养基组合物，用于大量生产具有免疫增强作用的蛋白质结合多糖。 构成：用于培养阴茎菌丝体的液体培养基组合物含有8〜16g / L的葡萄糖，20〜32g / L的果糖和4〜8g / L的大豆胨蛋白胨。 在液体培养基组合物上培养阳离子菌丝体的培养方法包括以下步骤：在琼脂培养基上培养阳离子斜方菌丝体; 在静止培养基上静置培养黑痣菌丝体; 并在液体培养基上液体培养阴离子菌体。
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公开(公告)号：KR1020110023416A
公开(公告)日：2011-03-08
申请号：KR1020090081286
申请日：2009-08-31
Applicant: 전북대학교산학협력단
Abstract: PURPOSE: A culture medium composition for cultivating inonotus obliquus mycelia is provided to mass-produce the inonotus obliquus mycelia and protein-bound polysaccharide with the anti-cancer and anti-diabetic effect. CONSTITUTION: A culture medium composition for cultivating inonotus obliquus mycelia contains 22g of glucose, 30g of oil, 6g of casein, 0g of soytone peptone, 3g of KH2PO4(calcium hydrogen phosphate), and 1L of distilled water. The oil is oleic acid. The inonotus obliquus mycelia are passage cultured on an agar medium, static cultured on a static cultivation medium, and liquid cultured on a liquid medium.
Abstract translation: 目的：提供一种用于培养斜颈菌丝体的培养基组合物，以大量生产具有抗癌和抗糖尿病作用的阴茎斜纹丝菌丝体和蛋白质结合多糖。 构成：用于培养斜颈菌丝体的培养基组合物含有22g葡萄糖，30g油，6g酪蛋白，0g大豆蛋白胨，3g KH 2 PO 4（磷酸氢钙）和1L蒸馏水。 油是油酸。 将inonotus obliquus菌丝体在琼脂培养基上培养，在静态培养基上静置培养，并在液体培养基上培养液体。
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公开(公告)号：KR1020090090855A
公开(公告)日：2009-08-26
申请号：KR1020080016373
申请日：2008-02-22
Applicant: (주)큐젠바이오텍 , 한국생산기술연구원 , 전북대학교산학협력단
Abstract: A method for culturing a Sparassis crispa mycelia is provided to massively produce Sparassis crispa mycelia and shorten culture time through semi-continuous culture of liquid phase. A medium for culturing Sparassis crispa mycelia in liquid phase comprises 0.01~3.0% of glucose, 0.01~0.5% of yeast extract, 0.01~0.2% malt extract, 0.01~1% of potassium monophosphate, and 0.01~0.04% of iron sulfate. A method for culturing the 0.01~0.04% in liquid phase comprises: a step of germinating a spore of Sparassis crispa mycelia; a step of culturing in liquid phase medium at 23‹C, 200 rpm of shaking speed for seven days; and a step of collecting beta-glucan from Sparassis crispa mycelia.
Abstract translation: 提供培养Sparassis crispa菌丝体的方法，大规模生产Sparassis crispa菌丝体，通过液相半连续培养缩短培养时间。 在液相中培养Sparassis crispa菌丝体的培养基包括0.01〜3.0％的葡萄糖，0.01〜0.5％的酵母提取物，0.01〜0.2％的麦芽提取物，0.01〜1％的单磷酸钾和0.01〜0.04％的硫酸铁。 一种在液相中培养0.01〜0.04％的方法，包括：将Sparassis crispa菌丝体孢子发芽的步骤; 在液相培养基中以23℃，200rpm振荡速度培养7天的步骤; 并从Sparassis crispa菌丝体中收集β-葡聚糖的步骤。
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Patent Agency Ranking
公开(公告)号：KR101119143B1
公开(公告)日：2012-03-19
申请号：KR1020090081286
申请日：2009-08-31
Applicant: 전북대학교산학협력단
Abstract: 본 발명은 항-당뇨 효능을 보이는 차가버섯 (
Inonotus
obliquus ) 유래 단백 다당체를 고농도로 생산하기 위한 차가버섯 균사체 고농도 생산용 액상배지 조성물 및 이를 이용한 차가버섯 균사체의 배양 방법에 관한 것이다. 보다 상세하게는, 탄소원으로서의 효율이 높은 오일 또는 올레인산이 첨가된 차가버섯 액상 배양용 배지 조성물 및 이를 이용한 균사체의 배양 방법에 관한 것으로, 본 발명은 종래의 차가버섯 균사체 액상 배지에 비해서 차가버섯 균사체 및 세포내 단백 다당체가 약 1.5 배 증가된 신규 산업용 액상 배지의 조성물이다.
차가버섯, 오일, 올레인산, 이노노토스 오블리쿠스, 액상배지-
公开(公告)号：KR1020110021156A
公开(公告)日：2011-03-04
申请号：KR1020090078782
申请日：2009-08-25
Applicant: 전북대학교산학협력단
Abstract: PURPOSE: A method for producing a large amount of extracellular proteoglycan derived from Inonotus obliquus is provided to apply to a pharmaceutical product and food product field. CONSTITUTION: A liquid culture medium for preparing extracellular proteoglycan from Inonotus obliquus mycelium and contains carbon source and nitrogen source in weight ratio of 6:1-9:1. The liquid culture medium is prepared by mixing with distilled water, adjusting at pH 5.0-6.0, and sterilizing at 110-130°C for 12-18 minutes.
Abstract translation: 目的：提供一种产生大量斜视白蛉的细胞外蛋白多糖的方法，适用于药品和食品领域。 构成：用于从Inonotus obliquus菌丝体制备细胞外蛋白多糖的液体培养基，含有碳源和氮源，重量比为6：1-9：1。 通过与蒸馏水混合制备液体培养基，调节pH为5.0-6.0，在110-130℃下灭菌12-18分钟。
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8.发明授权베타-1,6-분지-베타-1,3-글루칸을 고농도로 생산하는 치마버섯 신균주 큐지143-1 및 당해 균주를 사용한 베타-1,6-분지-베타-1,3-글루칸의 제조방법 有权베타-1,6- - 지 - -1타-1,3-을고농도로생산하는치마버섯신균주큐지143-1및균해균주를사용한베타-1,6분지 - 베타-1,3-글루칸의자조 방법
公开(公告)号：KR100909857B1
公开(公告)日：2009-07-29
申请号：KR1020080079595
申请日：2008-08-13
Applicant: (주)큐젠바이오텍 , 전북대학교산학협력단
Abstract: 본 발명은 베타-1,6-분지-베타-1,3-글루칸을 고농도로 생산하는 치마버섯 신균주에 관한 것으로, 보다 상세하게는 종래의 치마버섯에 비해 액체 배양에서 베타-1,6-분지-베타-1,3-글루칸의 생산성이 2.5배 높은 매우 우수한 신균주 큐지143-1 및 이로부터 베타-1,6-분지-베타-1,3-글루칸을 제조하는 방법을 제공한다.
베타-1,6-분지-베타-1,3-글루칸, QG143-1, 치마버섯 신균주, Schizophyllum communeAbstract translation: 提供了裂褶菌群QG143-1和使用它们的β-1,6-支链-β-1,3-葡聚糖的制备方法以提高β-1,6-支链-β-1,3-葡聚糖的生产率， 3-葡聚糖，与野生的裂褶菌群相比，并且在大量培养的情况下通过高细胞生长速率解决氧气和传质的不良影响。 Schizophyllum commune菌株是Schizophyllum commune QG143-1（登录号：KACC93060P）菌株。 Schizophyllum commune菌株对β-1,6-支链β-1,3-葡聚糖具有高生产力。 β-1,6-支链-β-1,3-葡聚糖是通过培养裂褶菌群落QG143-1菌株获得的。 培养是液体培养。
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9.发明公开베타-1,6-분지-베타-1,3-글루칸을 고농도로 생산하는 치마버섯 신균주 큐지143-1 및 당해 균주를 사용한 베타-1,6-분지-베타-1,3-글루칸의 제조방법 有权制造β-1,6-分支-β-1,3-GLUCAN的高浓度的SCHIZOPHYLLUM COMMUNE QG143-1以及使用其制备β-1,6-分支-β-1,3-GLUCAN的方法
公开(公告)号：KR1020090028403A
公开(公告)日：2009-03-18
申请号：KR1020080079595
申请日：2008-08-13
Applicant: (주)큐젠바이오텍 , 전북대학교산학협력단
Abstract: A Schizophyllum commune QG143-1 and a preparation method of beta-1,6-branched-beta-1,3-glucan, using the same are provided to increase the productivity of the beta-1,6-branched-beta-1,3-glucan, compared with wild Schizophyllum commune and to solve bad effects reaching oxygen and mass transfer by high cell growth rate in case of the mass culture. A Schizophyllum commune strain is the Schizophyllum commune QG143-1 (accession number : KACC93060P) strain. The Schizophyllum commune strain has high productivity for beta-1,6-branched-beta-1,3-glucan. The beta-1,6-branched-beta-1,3-glucan is obtained by cultivating the Schizophyllum commune QG143-1 strain. The cultivation is the liquid cultivation.
Abstract translation: 提供了裂殖酵母QG143-1和使用其的β-1,6-支链-β-1,3-葡聚糖的制备方法以提高β-1,6-支链-β-1,3-葡聚糖的生产率， 3-葡聚糖，与野生裂殖酵母菌相比，在大规模培养的情况下，通过高细胞生长速率解决达到氧气和质量传递的不良影响。 裂殖酵母菌株是裂殖酵母QG143-1（登录号：KACC93060P）菌株。 裂殖酵母菌株对β-1,6-支链-β-1,3-葡聚糖具有高生产力。 通过培养裂殖酵母菌QG143-1菌株获得β-1,6-支链-β-1,3-葡聚糖。 栽培是液体栽培。
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10.发明公开항균 및 진정 활성을 갖는 치마버섯 유래의베타-1,6-분지-베타-1,3-글루칸의 안용 조성물로써의 용도 无效β-1,6-BRANCHED-β-1,3-GLUCAN从SCHIZOPHYLLUM COMMUNE FR的应用 对于眼科解决方案的成分
公开(公告)号：KR1020090022691A
公开(公告)日：2009-03-04
申请号：KR1020070088262
申请日：2007-08-31
Applicant: (주)큐젠바이오텍 , 한국생산기술연구원 , 전북대학교산학협력단
IPC: A61K31/716 , A61P27/02
CPC classification number: A61K31/716 , A61K9/113 , A61K36/07 , A61K47/30 , Y10S514/912
Abstract: An ophthalmic liquid including beta-1,6-branched-beta-1,3-glucan derived from schizophyllum commune Fr. Is provided to maintain moisture in eyeballs and prevent ophthalmologic diseases. An ophthalmic liquid comprises beta-1,6-branched-beta-1,3-glucan derived from schizophyllum commune Fr., and a water-soluble polymeric compound. The water-soluble polymeric compound is selected from the group consisting of polyvinyl alcohol, polyvinylpyrrolidone, hydroxypropylmethylcellulose, methyl cellulose, polyethylene glycol, hydroxyethylenecellulose, and carboxyvinyl polymer. The concentration of the beta-1,6-branched-beta-1,3-glucan is 0.000001~99w/v%.
Abstract translation: 包括衍生于裂殖酵母菌株的β-1,6-支链-β-1,3-葡聚糖的眼科液 是为了保持眼球水分，防止眼科疾病。 眼科液体包含衍生自裂殖酵母属Fr.的β-1,6-支链-β-1,3-葡聚糖和水溶性聚合物。 水溶性高分子化合物选自聚乙烯醇，聚乙烯吡咯烷酮，羟丙基甲基纤维素，甲基纤维素，聚乙二醇，羟乙基纤维素和羧基乙烯基聚合物。 β-1,6-支链-β-1,3-葡聚糖的浓度为0.000001〜99w / v％。
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