公开(公告)号：KR100839585B1

公开(公告)日：2008-06-19

申请号：KR1020070035083

申请日：2007-04-10

Applicant: 조선대학교산학협력단

Inventor： 최철희 ,  이태범

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q2531/113 ,  C12Q2600/16

Abstract: A method and a kit for detecting an anti-cancer drug resistant gene are provided to detect various resistant genes through multiplex PCR using 5 primer sets capable of detecting 5 or 4 anti-cancer drug resistant gene simultaneously. A detection kit of an anti-cancer drug resistant gene using multiplex PCR is characterized in that it amplifies at least one gene set selected from the group consisting of: a gene set consisting of CFTR(cystic fibrosis transmembrane conductance regulator), ATP7B(Cupper transporting P-type ATPaseB), MVP(major vault protein), ABCC1(Multidrug Resistance Protein 1) and ABCG2[ATP-binding cassette, sub-family G(WHITE), member 2]; a gene set consisting of GAPD(glyceraldehyde-3-phosphate dehydrogenase), SLC28A1[solute carrier family 28(sodium-coupled nucleoside transporter), member 1], SLC9A1(Sodium/Hydrogen Exchanger 1) and ABCC11[ATP-binding cassette, sub-family C(CFTR/MRP), member 11]; a gene set consisting of ABCC6(ATP-binding cassette subfamily C member 6), ABCG1(ATP binding cassette transporter G1), ABCB4[ATP-binding cassette, sub-family B(MDR/TAP), member 4], ABCC4[ATP-binding cassette, sub-family C(CFTR/MRP), member 4] and SLC1A1[solute carrier family 31(copper transporters), member 1]; a gene set consisting of ABCC2[ATP-binding cassette, sub-family C(CFTR/MRP), member 2], ABCC5[ATP-binding cassette, subfamily C(CFTR/MRP), member 5], ABCC10[ATP-binding cassette, sub-family C(CFTR/MRP), member 10] and ABCC3[ATP-binding cassette, sub-family C(CFTR/MRP), member 3]; and a gene set consisting of SLC28A2[solute carrier family 28(sodium-coupled nucleoside transporter), member 2], ABCA2[solute carrier family 28(sodium-coupled nucleoside transporter), member 2], SLC29A2[solute carrier family 29(nucleoside transporters), member 2], ABCB1[ATP-binding cassette, sub-family B(MDR/TAP), member 1] and ABCA3[ATP-binding cassette, sub-family A(ABC1), member 3]. A method for detecting the anti-cancer drug resistant gene using multiplex PCR comprises a step of amplifying at least one set selected from the group consisting of: a set 1 consisting of a primer described as SEQ ID : NOs. 1 to 10; a set 2 consisting of a primer described as SEQ ID : NOs. 11 to 18; a set 3 consisting of a primer described as SEQ ID : NOs. 19 to 28; a set 4 consisting of a primer described as SEQ ID : NOs. 29 to 36; and a set 5 consisting of a primer described as SEQ ID : NOs. 37 to 46.

Abstract translation: 提供了一种用于检测抗癌药物抗性基因的方法和试剂盒，用于通过使用能够同时检测5或4种抗癌药物抗性基因的5种引物组的多重PCR检测各种抗性基因。 使用多重PCR的抗癌药物抗性基因的检测试剂盒的特征在于其扩增至少一种选自以下的基因集：由CFTR（囊性纤维化跨膜传导调节剂）组成的基因组，ATP7B（Cupper运输 P型ATP酶B），MVP（主要蛋白质蛋白），ABCC1（多药耐药蛋白1）和ABCG2 [ATP结合盒，亚家族G（WHITE），成员2]; 由GAPD（甘油醛-3-磷酸脱氢酶），SLC28A1 [溶质载体家族28（钠 - 偶联核苷转运蛋白），成员1]，SLC9A1（钠/氢交换器1）和ABCC11 [ATP结合盒，子 - 家族C（CFTR / MRP），成员11]; 由ABCC6（ATP结合盒亚家族C成员6），ABCG1（ATP结合盒转运蛋白G1），ABCB4 [ATP结合盒，亚B族（MDR / TAP），成员4]，ABCC4 [ATP 结合盒，亚科C（CFTR / MRP），成员4]和SLC1A1 [溶质载体家族31（铜转运蛋白），成员1]; 由ABCC2 [ATP结合盒，亚类C（CFTR / MRP），成员2]，ABCC5 [ATP结合盒，亚科C（CFTR / MRP），成员5]，ABCC10 [ATP结合 盒子，亚科C（CFTR / MRP），成员10]和ABCC3 [ATP结合盒，子系C（CFTR / MRP），成员3]; 和由SLC28A2 [溶质载体家族28（钠偶联核苷转运蛋白），成员2]，ABCA2 [溶质载体家族28（钠 - 偶联核苷转运蛋白），成员2]，SLC29A2 [溶质载体家族29（核苷 转运蛋白），成员2]，ABCB1 [ATP结合盒，亚家族B（MDR / TAP），成员1]和ABCA3 [ATP结合盒，亚家族A（ABC1），成员3]。 使用多重PCR检测抗癌药物抗性基因的方法包括扩增选自以下的至少一组的步骤：由SEQ ID NO：所示的引物组成的组1。 1至10; 由SEQ ID NO：所示引物组成的组2。 11至18; 由SEQ ID NO：所示的引物组成的组3。 19到28; 由SEQ ID NO：所示的引物组成的组4。 29至36; 和由SEQ ID NO：所示的引物组成的集合5。 37到46。