Abstract:
본 발명은 백신으로 사용할 수 있는 돼지생식기 및 호흡기 증후군(PRRS) 바이러스의 키메라 바이러스에 관한 것이다. 본 발명의 PRRSV 키메라 바이러스는 모균주보다 약독화되어 있어 병원성이 낮고 안정성이 높으면서, 중화항체의 분비를 향상시켜 PRRS 질환의 효과적인 예방 및 치료용 백신으로 사용할 수 있다.
Abstract:
본 발명은 유전체의 특정 위치에 코돈쌍 탈최적화를 통한 침묵 돌연변이가 도입된 유럽형 돼지 생식기 호흡기 증후군 바이러스의 변이주 및 이의 용도에 관한 것이다. 본 발명이 제공하는 바이러스 변이주는 야생형 균주에 비해 약독화되었으며, 돼지 체내에서의 증식성이 저하되어 유럽형 돼지 생식기 호흡기 증후군 예방용 백신으로 활용될 수 있다.
Abstract:
PURPOSE: A primer and kit for detection and pathotype-diagnosis of Newcastle disease virus are provided, thereby simultaneously detecting the Newcastle disease virus and diagnosing pathotype of the Newcastle disease virus, so that death rate of domestic animals by the Newcastle disease virus can be reduced. CONSTITUTION: The primer for detection and pathotype-diagnosis of Newcastle disease virus is selected from a primer set having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2, a primer set having the nucleotide sequences of SEQ ID NO:2 and SEQ ID NO:3, and a primer set having the nucleotide sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3. The method for pathotype-diagnosis of Newcastle disease virus comprises the steps of: (1) obtaining RNA from a sample; (2) carrying out reverse transcription of the RNA to obtain cDNA; (3) PCR amplifying the cDNA using the primer; (4) subjecting the PCR product to electrophoresis; and (5) deciding the Newcastle disease virus as non-pathogenic virus when the PCR product shows a band of 379bp, or as pathogenic virus when the PCR product shows two bands of 379bp and 204bp.
Abstract:
PURPOSE: A nucleocapsid gene of infectious bronchitis virus is provided, which gene is useful as a molecular marker for determining and classifying the type of infectious bronchitis virus. CONSTITUTION: The nucleocapsid gene of infectious bronchitis virus has the nucleotide sequence set forth in SEQ ID NO:1, wherein the nucleocapsid gene is selected from the nucleotide sequences set forth in SEQ ID NO:2 to SEQ ID NO:39. The method for determining the type of infectious bronchitis virus comprises the steps of: (1) PCR amplifying a testing DNA using primers having the nucleotide sequences set forth in SEQ ID NO:40 and SEQ ID NO:41; (2) sequencing the PCR amplified product; and (3) comparing the sequenced nucleotide sequence with the nucleocapsid gene of SEQ ID NO:1.
Abstract translation:目的:提供感染性支气管炎病毒的核衣壳基因,该基因可用作确定和分类感染性支气管炎病毒类型的分子标记物。 构成:感染性支气管炎病毒的核衣壳基因具有SEQ ID NO:1所示的核苷酸序列,其中核衣壳基因选自SEQ ID NO:2〜SEQ ID NO:39所示的核苷酸序列。 用于确定感染性支气管炎病毒类型的方法包括以下步骤:(1)使用具有SEQ ID NO:40和SEQ ID NO:41所示核苷酸序列的引物PCR扩增测试DNA; (2)对PCR扩增产物进行测序; 和(3)将测序的核苷酸序列与SEQ ID NO:1的核衣壳基因进行比较。
Abstract:
PURPOSE: Reverse transcriptase from Reticuloendotheliosis virus and a mass-production method thereof are provided. The enzyme is useful for diagnosis of disease or gene cloning by synthesis of cDNA from RNA. CONSTITUTION: The reverse transcriptase from Reticuloendotheliosis virus has the amino acid sequence set forth in SEQ ID NO:2. A gene encoding the reverse transcriptase from Reticuloendotheliosis virus has the nucleotide sequence set forth in SEQ ID NO:1. The method for mass-producing the reverse transcriptase from Reticuloendotheliosis virus comprises the steps of: constructing a vector containing the reverse transcriptase gene of SEQ ID NO:1; transforming Escherichia coli with the vector to produce a transformed E. coli; culturing the transformed E. coli in a medium; isolating cultured cells containing the expressed recombinant Reticuloendotheliosis virus reverse transcriptase; dissolving the isolated cells in a denaturation buffer solution containing urea or guanidine chloride; and reacting the dissolved protein with a renaturation buffer solution to refold the protein.
Abstract:
PURPOSE: Provided are primers for detection of newcastle disease virus, and methods for diagnosing the newcastle disease by simultaneously performing detection and pathotyping thereof and for genotyping of the newcastle disease virus by RFLP(restriction fragment length polymorphism). CONSTITUTION: The primers for detection of newcastle disease virus are characteristically represented by the SEQ ID NO:1 and 2. The primers for pathotyping of the newcastle disease virus are characteristically represented by the SEQ ID NO:3 and 4. The method for genotyping of the newcastle disease virus is characterized by RFLP using Hinf I and Pst I as restriction enzymes, and PCR products using the primers of SEQ ID NO:1 and 2.
Abstract translation:目的:提供了用于检测新城疫病毒的引物,以及通过同时检测和分型以及用RFLP(限制性片段长度多态性)对新城疫病毒进行基因分型来诊断新城疫的方法。 构成:用于检测新城疫病毒的引物的特征在于SEQ ID NO:1和2.用于新城疫病毒的pathotyping的引物的特征在于SEQ ID NO:3和4.用于基因分型的方法 新城疫病毒的特征在于使用Hinf I和Pst I作为限制性酶的RFLP,以及使用SEQ ID NO:1和2的引物的PCR产物。
Abstract:
PURPOSE: F(fusion) protein and HN(haemagglutinin-neuraminidase) protein of Newcastle disease virus strains and genes thereof are provided which are used as marker molecules for diagnosing newcastle disease virus and for development of a vaccine therefor. CONSTITUTION: The F protein consists of amino acids represented by one of SEQ ID NOs:14 to 26. F protein gene consists of nucleotide sequence represented by one of SEQ ID NOs: 1-13. The HN protein consists of amino acids represented by one of SEQ ID NOs:34 to 40. HN protein gene consists of nucleotide sequence represented by one of SEQ ID NOs: 27-33.
Abstract translation:目的:提供新城疫病毒株的F(融合)蛋白质和HN(血细胞凝集素 - 神经氨酸酶)蛋白质及其基因,其用作诊断新城疫病毒的标志物分子并用于开发其疫苗。 构成:F蛋白由SEQ ID NO:14至26中的一个表示的氨基酸组成.F蛋白基因由SEQ ID NO:1-13之一表示的核苷酸序列组成。 HN蛋白由SEQ ID NO:34至40之一表示的氨基酸组成.HN蛋白基因由SEQ ID NO:27-33之一所示的核苷酸序列组成。
Abstract:
본 발명은 서열번호 1 로 기재되는 전염성 기관지염 바이러스(Infectious bronchitis virus)의 뉴클레오캡시드(Nucleocapsid) 유전자에 관한 것으로서, 보다 상세하게는 서열번호 2 내지 서열번호 39 로 기재되는 염기서열을 갖는 전염성 기관지염 바이러스의 뉴클레오캡시드 유전자 및 상기 유전자를 이용하여 전염성 기관지염 바이러스의 타입을 결정하는 방법에 관한 것이다. 본 발명의 뉴클레오캡시드 유전자는 전염성 기관지염 바이러스의 타입 결정 및 분류를 위한 분자 마커로 유용하게 사용될 수 있다.