Abstract:
PURPOSE: A support and gene array for in situ generation of protein array is provided to quickly convert various kinds of DNA or mRNA to active protein of array form and enhance protein array analysis sensitivity. CONSTITUTION: A support for in situ generation of protein array contains a solid support and hydrogel which is coated on the solid support and comprise protein biosynthesis machinery. The hydrogel is agarose gel. The hydrogel is manufactured by spin coating in a film form. A gene array for in situ generation of protein array comprises the support for in situ generation of protein array and a genetic template spotted on the support.