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Patent Agency Ranking
公开(公告)号：KR100836833B1
公开(公告)日：2008-06-11
申请号：KR1020060055689
申请日：2006-06-21
Applicant: 충남대학교산학협력단
Abstract: 본 발명은 남세균 시네코시스티스 (
Synechocystis
sp.) PCC6803으로부터의 포도당 유도성 유전자의 프로모터 부위를 포함하는 미생물을 이용한, 포도당 유도성 바이오센서에 관한 것으로서, 본 발명에 따른 바이오센서는 포도당에 민감하게 반응할 뿐만 아니라, 에너지대사를 교란하는 환경독성 물질, 예를 들어, 중금속, 시안, 제초제, 유전자 전사·번역 억제제, 및 기준치 농도의 위해물질도 검출할 수 있으므로, 포도당과 같은 영양물질이나 에너지대사 교란물질을 검출하거나 유전자 발현을 억제하는 항생제의 생체검정 및 세포 어레이 제작에도 사용됨으로써, 제약산업, 항생제나 제초제와 같은 신약개발이나 수생태계 환경독성 물질 검출 및 잔류농약이나 중금속의 실시간·비파괴적 검출에 유용하게 활용될 수 있다.
포도당 유도성 유전자, 프로모터, 남세균, 시아노박테리아, 발광유전자, 환경독성, 바이오센서-
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3.发明公开
公开(公告)号：KR1020090131310A
公开(公告)日：2009-12-29
申请号：KR1020080057116
申请日：2008-06-18
Applicant: 충남대학교산학협력단
Abstract: PURPOSE: A composition for controlling cell size of Synechocystis sp. containing cya1 and cya2 genes is provided to suppress respiration of Synechocystis sp. and control cell size. CONSTITUTION: A composition for controlling cell size of Synechocystis sp. contains cya 1(adenylate cyclase) and cya 2(guanylate cyclase genes). The genes comprise sequence of sequence numbers 1 and 2. The Synechocystis sp. is Synechocystis sp. PCC6803 strain. The cya 1 and cya 2 genes suppress respiration to glucose metabolism of Synechocystis sp. The composition causes overexpression of cya 1 and cya 2 genes so the cell size of the Synechocystis sp. is increased. The cya 1 and cya 2 genes are deleted in Synechocystis sp. PCC6803 mutant cell line.
Abstract translation: 目的：用于控制集胞藻的细胞大小的组合物 提供含有cya1和cya2基因以抑制集胞藻的呼吸。 并控制细胞大小。 构成：用于控制集胞藻的细胞大小的组合物 含有cya 1（腺苷酸环化酶）和cya 2（鸟苷酸环化酶基因）。 基因包含序列号1和序列2。 是集胞藻（Synechocystis sp。） PCC6803菌株。 cya 1和cya 2基因抑制呼吸作用对集胞藻的葡萄糖代谢。 该组合物导致cya 1和cya 2基因的过表达，因此集胞藻属的细胞大小。 增加了。 cya 1和cya 2基因在Synechocystis sp。中被删除。 PCC6803突变体细胞系。
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4.发明公开
公开(公告)号：KR1020090131309A
公开(公告)日：2009-12-29
申请号：KR1020080057115
申请日：2008-06-18
Applicant: 충남대학교산학협력단
Abstract: PURPOSE: An svr1(synechocystis 4-vinyl reductase 1) is provided to control cell size of cyanobacteria and research cell size regulation of other bacteria. CONSTITUTION: An svr1(synechocystis 4-vinyl reductase 1) regulates cell size of Synechocystis sp. The svr1 gene comprises a sequence of sequence numbr 1. A host cells are transformed using a recombinant vector containing svr1 gene. The host cell is Synechocystis sp. PCC6803. The cell size of the Synechocystis sp. is regulated by expressing svr1 gene. When the svr1 gene is overexpressed, the cell size of Synechocystis sp. is reduced. When the expression of svr1 is suppressed, the cell size of Synechocystis sp. is increased.
Abstract translation: 目的：提供svr1（synechocystis 4-乙烯基还原酶1）以控制蓝细菌的细胞大小，并研究其他细菌的细胞大小调节。 构成：svr1（synechocystis 4-乙烯基还原酶1）调节集胞藻的细胞大小。 svr1基因包含序列号1的序列。使用含有svr1基因的重组载体转化宿主细胞。 宿主细胞是集胞藻（Synechocystis sp。 6803。 集胞藻的细胞大小 通过表达svr1基因来调控。 当svr1基因过表达时，集胞藻的细胞大小 降低了。 当svr1的表达被抑制时，集胞藻的细胞大小 增加了。
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公开(公告)号：KR1020070121088A
公开(公告)日：2007-12-27
申请号：KR1020060055689
申请日：2006-06-21
Applicant: 충남대학교산학협력단
CPC classification number: C12Q1/6897 , C12Q1/6825 , G01N33/5308
Abstract: A biosensor is provided to be used for screening nutritive materials such as glucose, heavy metals or cyanogen or be applied to biopsy of herbicides or antibiotics, thereby being usefully employed to develop new drugs, detect environment-toxic materials of aquatic ecosystem, and screen residual pesticides and heavy metals at real-time in a non-destructive way. A glucose inducible biosensor comprises a microorganism including a promoter portion of a glucose inducible gene derived from Synechocystis sp. PCC6803, which is selected from the group consisting of atp I, ndbA, catD1, tkt, pgi, pdh, ppc and cydA. To screen glucose, environment-toxic materials, inhibitors of transcription or translation or harmful materials having the concentration over reference value, the biosensor is used.
Abstract translation: 提供生物传感器用于筛选葡萄糖，重金属或氰的营养物质，或用于除草剂或抗生素的活检，从而有效地用于开发新药，检测水生生态系统的环境毒性物质，筛选残留物 农药和重金属以非破坏性的方式实时传播。 葡萄糖诱导型生物传感器包括微生物，其包含源自集胞藻（Synechocystis sp。）的葡萄糖诱导型基因的启动子部分。 PCC6803，其选自atp I，ndbA，catD1，tkt，pgi，pdh，ppc和cydA。 为了筛选葡萄糖，环境毒性物质，转录或翻译抑制剂或浓度超过参考值的有害物质，使用生物传感器。
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