公开(公告)号：KR1020100077508A

公开(公告)日：2010-07-08

申请号：KR1020080135463

申请日：2008-12-29

Applicant: 충남대학교산학협력단

Inventor： 황성주 ,  김승수 ,  박찬혁

IPC: A61K9/127 ,  A61K31/7048

CPC classification number: A61K9/1277 ,  A61K31/7048 ,  A61K47/24 ,  A61K47/28

Abstract: PURPOSE: A method for manufacturing amphotericin B-containing liposome is provided to enhance inclusion efficiency of amphotericin B and to obtain liposome with formulation stability. CONSTITUTION: A method for manufacturing amphotericin B-containing liposome comprises: a step of preparing mixture solution having amphotericin B, phospholipid, and sterol to obtain mixture solution; a step of mixing aqueous phase having water and a supercritical fluid together with the mixture solution to obtain emulsion of supercritical fluid/aqueous phase; and a step of discharging supercritical fluid by decompression to form liposome solution. The solvent for making the mixture solution is chloroform, methanol, ethanol, ether, or mixture thereof.

Abstract translation: 目的：提供一种制备两性霉素B的脂质体的方法，以提高两性霉素B的包合效率，并获得具有制剂稳定性的脂质体。 构成：含两性霉素B的脂质体的制造方法，包括：制备具有两性霉素B，磷脂和固醇的混合溶液的步骤，得到混合溶液; 将具有水和超临界流体的水相与混合溶液混合以获得超临界流体/水相的乳液的步骤; 以及通过减压排出超临界流体以形成脂质体溶液的步骤。 用于制备混合溶液的溶剂是氯仿，甲醇，乙醇，乙醚或其混合物。