公开(公告)号：KR1020020060100A

公开(公告)日：2002-07-16

申请号：KR1020020001823

申请日：2002-01-08

Applicant: 충남대학교산학협력단

Inventor： 김철중 ,  김은 ,  신광순 ,  김현수

IPC: C12N15/86

CPC classification number: C12N15/86 ,  C12N5/0686 ,  C12N2740/16023 ,  C12N2740/16051 ,  C12N2770/36141 ,  G01N33/536 ,  G01N33/56988 ,  G01N33/6854 ,  G01N2333/16

Abstract: PURPOSE: Provided is HIV like particle which is composed of HIV-1 Gag, Env, Pol and/or Pro proteins expressed by an expression vector containing HIV-1 env, gag, pro, pol genes. The HIV like particle can be used as an antigen in a diagnosis kit of HIV infection and for HIV vaccine composition. CONSTITUTION: Alphavirus based expression vectors contain HIV-1 env, gag, pro and pol genes and they are as follows: pSFV/env-gag, pSFV/gag-env, pSFV/env-gag-pro, pSFV/env-gag-gagpro, pSFV/env-gag-gagΔpro, and pSFV/gagpol-env. The expression vectors further contains CTE(constitutive transport element) gene and they are as follows: pSFV/env-gag-gagpro-CTE, pSFV/env-gag-gagΔpro-CTE, pSFV/gagpol-env-MCTE, and pSF/gag-env-MCTE. HIV-like particle is manufactured by introducing one of the above vectors or its RNA transcript into an animal cell then expressing it.

Abstract translation: 目的：提供由含有HIV-1 env，gag，pro，pol基因的表达载体表达的由HIV-1Gag，Env，Pol和/或Pro蛋白组成的HIV样颗粒。 HIV样颗粒可用作HIV感染和HIV疫苗组成的诊断试剂盒中的抗原。 构成：基于甲病毒基因的表达载体含有HIV-1 env，gag，pro和pol基因，它们如下：pSFV / env-gag，pSFV / gag-env，pSFV / env-gag-pro，pSFV / env-gag- gagpro，pSFV / env-gag-gagΔpro和pSFV / gagpol-env。 表达载体还含有CTE（组成型转运元件）基因，它们如下：pSFV / env-gag-gagpro-CTE，pSFV / env-gag-gagΔpro-CTE，pSFV / gagpol-env-MCTE和pSF / gag -env-MCTE。 通过将上述载体之一或其RNA转录物引入动物细胞中然后进行表达来制备HIV样颗粒。

公开(公告)号：KR100522448B1

公开(公告)日：2005-11-15

申请号：KR1020020001823

申请日：2002-01-08

Applicant: 충남대학교산학협력단

Inventor： 김철중 ,  김은 ,  신광순 ,  김현수

IPC: C12N15/86