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公开(公告)号：KR1020030013585A
公开(公告)日：2003-02-15
申请号：KR1020010047663
申请日：2001-08-08
Applicant: 충남대학교산학협력단
IPC: C12N15/86
CPC classification number: C12N7/02 , C12N5/067 , C12N15/86 , C12N2770/24223 , C12N2770/24251 , C12N2770/36141
Abstract: PURPOSE: Provided is a preparation of infectious HCV-like particles which are characteristically used as antigens for the detection HCV vaccine or HCV antibodies and for the inspection or examination of infection inhibiting drugs. CONSTITUTION: The preparation of infectious HCV-like particles is characterized by using a recombinant alphavirus expression vector(KCCM-10287) and a helper vector(KCCM-10286) which express HCV core, E1 and E2.
Abstract translation: 目的：提供特征性地用作检测HCV疫苗或HCV抗体的抗原以及感染抑制药物的检查或检查的感染性HCV样颗粒的制剂。 构成：感染性HCV样颗粒的制备的特征在于使用表达HCV核心，E1和E2的重组甲病毒表达载体（KCCM-10287）和辅助载体（KCCM-10286）。
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Patent Agency Ranking
公开(公告)号：KR100457790B1
公开(公告)日：2004-12-08
申请号：KR1020010047663
申请日：2001-08-08
Applicant: 충남대학교산학협력단
IPC: C12N15/86
Abstract: PURPOSE: Provided is a preparation of infectious HCV-like particles which are characteristically used as antigens for the detection HCV vaccine or HCV antibodies and for the inspection or examination of infection inhibiting drugs. CONSTITUTION: The preparation of infectious HCV-like particles is characterized by using a recombinant alphavirus expression vector(KCCM-10287) and a helper vector(KCCM-10286) which express HCV core, E1 and E2.
Abstract translation: 目的：提供一种感染性HCV样颗粒的制备物，其特征性地用作检测HCV疫苗或HCV抗体的抗原以及检测或检查感染抑制药物。 构成：通过使用表达HCV核心，E1和E2的重组α病毒表达载体（KCCM-10287）和辅助载体（KCCM-10286）来表征感染性HCV样颗粒的制备。
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公开(公告)号：KR1020020067833A
公开(公告)日：2002-08-24
申请号：KR1020010008201
申请日：2001-02-19
Applicant: 충남대학교산학협력단
IPC: C12N15/86
CPC classification number: C12N15/86 , C07K14/4703 , C07K14/4746 , C12N5/0693
Abstract: PURPOSE: An Alphavirus expression vector capable of expressing a cancer-inhibiting protein, its producing method and its use are provided, thereby effectively inhibiting and preventing the growth of a cancer cell. CONSTITUTION: The Alphavirus expression vector capable of expressing a cancer-inhibiting protein contains a first cancer-inhibiting gene which is operably linked under operation of a first subgenomic promoter, a second cancer-inhibiting gene which is operably linked under operation of a second subgenomic promoter, and a third cancer-inhibiting gene which is operably linked under operation of a third subgenomic promoter, in which the cancer-inhibiting gene is selected from pRb/p110 or its homologue p107 and p120, p53 protein, p21 protein, p21 protein(WAF-1), bak, BRCA1, BRCA2, PTEN, APC, DCC, NF-1, NF-2, WT-1, MEN-I, MEN-II, zac1, p73, VHL, FCC and MCC; and the Alphavirus is Sindbis virus or Semliki Forest virus(SFV).
Abstract translation: 目的：提供能够表达癌抑制蛋白的甲病毒表达载体及其制备方法及其用途，从而有效抑制和预防癌细胞的生长。 构成：能够表达癌症抑制蛋白的甲病毒载体表达载体含有第一癌抑制基因，其在第一亚基因组启动子的操作下可操作地连接，第二癌抑制基因在第二亚基因组启动子的操作下可操作地连接 ，以及第三癌抑制基因，其在第三亚基因组启动子的操作下可操作地连接，其中所述癌抑制基因选自pRb / p110或其同源物p107和p120，p53蛋白，p21蛋白，p21蛋白（WAF -1），bak，BRCA1，BRCA2，PTEN，APC，DCC，NF-1，NF-2，WT-1，MEN-1，MEN-II，zac1，p73，VHL，FCC和MCC; 甲病毒属于辛德毕斯病毒或辛格利奇森林病毒（SFV）。
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公开(公告)号：KR1020020060100A
公开(公告)日：2002-07-16
申请号：KR1020020001823
申请日：2002-01-08
Applicant: 충남대학교산학협력단
IPC: C12N15/86
CPC classification number: C12N15/86 , C12N5/0686 , C12N2740/16023 , C12N2740/16051 , C12N2770/36141 , G01N33/536 , G01N33/56988 , G01N33/6854 , G01N2333/16
Abstract: PURPOSE: Provided is HIV like particle which is composed of HIV-1 Gag, Env, Pol and/or Pro proteins expressed by an expression vector containing HIV-1 env, gag, pro, pol genes. The HIV like particle can be used as an antigen in a diagnosis kit of HIV infection and for HIV vaccine composition. CONSTITUTION: Alphavirus based expression vectors contain HIV-1 env, gag, pro and pol genes and they are as follows: pSFV/env-gag, pSFV/gag-env, pSFV/env-gag-pro, pSFV/env-gag-gagpro, pSFV/env-gag-gagΔpro, and pSFV/gagpol-env. The expression vectors further contains CTE(constitutive transport element) gene and they are as follows: pSFV/env-gag-gagpro-CTE, pSFV/env-gag-gagΔpro-CTE, pSFV/gagpol-env-MCTE, and pSF/gag-env-MCTE. HIV-like particle is manufactured by introducing one of the above vectors or its RNA transcript into an animal cell then expressing it.
Abstract translation: 目的：提供由含有HIV-1 env，gag，pro，pol基因的表达载体表达的由HIV-1Gag，Env，Pol和/或Pro蛋白组成的HIV样颗粒。 HIV样颗粒可用作HIV感染和HIV疫苗组成的诊断试剂盒中的抗原。 构成：基于甲病毒基因的表达载体含有HIV-1 env，gag，pro和pol基因，它们如下：pSFV / env-gag，pSFV / gag-env，pSFV / env-gag-pro，pSFV / env-gag- gagpro，pSFV / env-gag-gagΔpro和pSFV / gagpol-env。 表达载体还含有CTE（组成型转运元件）基因，它们如下：pSFV / env-gag-gagpro-CTE，pSFV / env-gag-gagΔpro-CTE，pSFV / gagpol-env-MCTE和pSF / gag -env-MCTE。 通过将上述载体之一或其RNA转录物引入动物细胞中然后进行表达来制备HIV样颗粒。
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公开(公告)号：KR100457791B1
公开(公告)日：2004-11-18
申请号：KR1020010008201
申请日：2001-02-19
Applicant: 충남대학교산학협력단
IPC: C12N15/86
Abstract: PURPOSE: An Alphavirus expression vector capable of expressing a cancer-inhibiting protein, its producing method and its use are provided, thereby effectively inhibiting and preventing the growth of a cancer cell. CONSTITUTION: The Alphavirus expression vector capable of expressing a cancer-inhibiting protein contains a first cancer-inhibiting gene which is operably linked under operation of a first subgenomic promoter, a second cancer-inhibiting gene which is operably linked under operation of a second subgenomic promoter, and a third cancer-inhibiting gene which is operably linked under operation of a third subgenomic promoter, in which the cancer-inhibiting gene is selected from pRb/p110 or its homologue p107 and p120, p53 protein, p21 protein, p21 protein(WAF-1), bak, BRCA1, BRCA2, PTEN, APC, DCC, NF-1, NF-2, WT-1, MEN-I, MEN-II, zac1, p73, VHL, FCC and MCC; and the Alphavirus is Sindbis virus or Semliki Forest virus(SFV).
Abstract translation: 目的：提供一种能够表达癌症抑制蛋白的甲病毒表达载体，其生产方法及其用途，由此有效地抑制和预防癌细胞的生长。 构成：能够表达癌症抑制蛋白的甲病毒表达载体含有第一种癌症抑制基因，该基因在第一亚基因组启动子的操作下可操作地连接，第二癌基因是在第二亚基因组启动子操作下可操作地连接的 和在第三亚基因组启动子的操作下可操作连接的第三癌抑制基因，其中所述癌抑制基因选自pRb / p110或其同源物p107和p120，p53蛋白，p21蛋白，p21蛋白（WAF -1），bak，BRCA1，BRCA2，PTEN，APC，DCC，NF-1，NF-2，WT-1，MEN-1，MEN-II，zac1，p73，VHL，FCC和MCC; 而α病毒是辛德毕斯病毒或塞姆利基森林病毒（SFV）。
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8.发明公开암억제 단백질을 발현하는 알파바이러스계 발현 벡터,이의 제조 방법 및 용도 有权基于ALPHAVIRUS的表达载体表达肿瘤抑制蛋白，其制备方法及其用途同时显示各种肿瘤抑制蛋白和有效抑制肿瘤的作用
公开(公告)号：KR1020040077600A
公开(公告)日：2004-09-04
申请号：KR1020040060593
申请日：2004-07-30
Applicant: 충남대학교산학협력단
CPC classification number: A61K48/00 , A61K2039/585 , C12N15/85
Abstract: PURPOSE: Alphavirus-based expression vectors expressing tumor suppressor proteins, a method for preparing the same and the use thereof are provided, thereby simultaneously expressing various tumor suppressor proteins, and consequently suppressing tumor effectively. CONSTITUTION: The alphavirus-based expression vector expressing tumor suppressor proteins comprises a first tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a first subgenomic promoter; a second tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a second subgenomic promoter; and a third tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a third subgenomic promoter, wherein the first, second and third tumor suppressing genes or angiogenesis suppressing genes are the same or different; the tumor suppressing gene is p53 gene or PTEN gene; the antigiogenesis suppressing gene is angiostatin gene; and the alphavirus-based expression vector expresses tumor suppressor proteins Semliki forest virus(SFV) and Sindbis virus.
Abstract translation: 目的：提供表达肿瘤抑制蛋白的基于甲病毒的表达载体，其制备方法及其用途，同时表达各种肿瘤抑制蛋白，从而有效抑制肿瘤。 构成：表达肿瘤抑制蛋白的基于甲病毒的表达载体包含与第一亚基因组启动子有效连接的第一肿瘤抑制基因或血管发生抑制基因; 与第二亚基因组启动子可操作地连接的第二肿瘤抑制基因或血管发生抑制基因; 和与第三亚基因组启动子可操作地连接的第三肿瘤抑制基因或血管发生抑制基因，其中第一，第二和第三肿瘤抑制基因或血管发生抑制基因相同或不同; 肿瘤抑制基因是p53基因或PTEN基因; 抗血管生成抑制基因是血管抑素基因; 并且基于甲病毒的表达载体表达肿瘤抑制蛋白Semliki森林病毒（SFV）和辛德毕斯病毒。
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