公开(公告)号：KR1020140107804A

公开(公告)日：2014-09-05

申请号：KR1020130021817

申请日：2013-02-28

Applicant: 충남대학교산학협력단

Inventor： 김민규 ,  김근중 ,  이지혜 ,  김은영 ,  김마리아 ,  김종상 ,  김홍주 ,  임재삼 ,  백준종 ,  이정웅

IPC: C12N15/65 ,  C12N15/62 ,  C12N5/10 ,  A01K67/027

Abstract: The present invention, as a method for mass-producing medicine to treat rheumatoid arthritis, relates to a method for producing medicine by expressing gene from mammary gland of a transformed animal using expression vector including base sequence specifically expressed and controlled in mammary gland of a pig. More specifically, the present invention is production of a pig, a transformed animal through somatic cell clone after making recombinant protein to mass-produce TNF-R fusion protein and then transfecting the recombinant protein into a somatic cell of a pig.

Abstract translation: 作为用于大规模生产药物治疗类风湿性关节炎的方法本发明涉及通过使用包括在猪的乳腺中特异表达和控制的碱基序列的表达载体来表达转化动物的乳腺基因来生产药物的方法 。 更具体地说，本发明是在制备重组蛋白质大规模生产TNF-R融合蛋白后，通过体细胞克隆生产转化动物，然后将重组蛋白转染到猪的体细胞中。