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    Ｍｙｏ－２ 펩타이드 중합체와 마이오스타틴의 융합단백질표면발현용 벡터 및 상기 벡터로 형질전환된 미생물
    7.
    发明授权
    Ｍｙｏ－２ 펩타이드 중합체와 마이오스타틴의 융합단백질표면발현용 벡터 및 상기 벡터로 형질전환된 미생물 有权
    MYO-2肽多聚体和肌醇六磷酸融合蛋白的表面表达载体及其转化的微生物

    公开(公告)号：KR100857861B1

    公开(公告)日：2008-09-11

    申请号：KR1020070103512

    申请日：2007-10-15

    Applicant: 주식회사 바이오리더스 ,  한국생명공학연구원 ,  충남대학교산학협력단

    Inventor： 성문희 ,  김철중 ,  부하령 ,  김지연 ,  김영숙 ,  허룡춘

    IPC: C07K16/00 ,  C07K19/00

    CPC classification number: C07K14/475

    Abstract: A surface expression vector for fusion protein of Myo-2 peptide multimer and myostatin is provided to express the myostatin fusion protein on the surface of bacteria, and increase the blood antibody production, body weight and muscle amount of animals by orally administering the bacteria. A myostatin derived peptide Myo-2 has the amino acid sequence of SEQ ID NO:1. A Myo-2 peptide multimer is prepared by polymerizing 2-8 Myo-2 peptides. A fusion protein is prepared by fusing the Myo-2 peptide multimer with matured myostatin. A surface expression vector for fusion protein contains a gene encoding polygammaglutamic acid synthetase complex selected from pgsB(polygammaglutamic acid synthetase), pgsC and pgsA, the nucleotide sequence encoding the Myo-2 peptide multimer and a gene encoding the matured myostatin. A method for producing a fusion protein-surface expressed microorganism comprises the steps of: (a) expressing the fusion protein of myostatin derived peptide multimer and matured myostatin on the surface of microorganism by culturing a microorganism transformed with the surface expression vector; and (b) recovering the microorganism with surface expressed matured myostatin, wherein the microorganism is lactic acid bacterium. Further, the matured myostatin is be derived from mammal or birds.

    Abstract translation: 提供Myo-2肽多聚体和肌生成抑制素融合蛋白的表达表达载体，以表达细菌表面的肌生成抑制素融合蛋白，并通过口服施用细菌增加动物的血液抗体产生，体重和肌肉量。 肌生长抑制素衍生肽Myo-2具有SEQ ID NO：1的氨基酸序列。 通过聚合2-8个Myo-2肽制备Myo-2肽多聚体。 通过将Myo-2肽多聚体与成熟的肌生成抑制素融合来制备融合蛋白。 用于融合蛋白的表面表达载体包含编码聚腺苷酸合成酶复合物的基因，其选自pgsB（polygammaglutamic acid synthetase），pgsC和pgsA，编码Myo-2肽多聚体的核苷酸序列和编码成熟肌肉生长抑制素的基因。 一种融合蛋白表面表达微生物的制备方法，包括以下步骤：（a）通过培养用表面表达载体转化的微生物，将肌生长抑制素衍生肽多聚体和成熟肌生成抑制素的融合蛋白表达在微生物表面上; 和（b）用表面表达成熟的肌生长抑制素回收微生物，其中微生物是乳酸菌。 此外，成熟的肌生成抑制素来源于哺乳动物或鸟类。

    암억제 단백질을 발현하는 알파바이러스계 발현 벡터, 이의 제조 방법 및 용도
    8.
    发明授权
    암억제 단백질을 발현하는 알파바이러스계 발현 벡터, 이의 제조 방법 및 용도 有权
    암억제단백질을발현하는알파바이러스계발현벡터，이의제조법법및및용도

    公开(公告)号：KR100457791B1

    公开(公告)日：2004-11-18

    申请号：KR1020010008201

    申请日：2001-02-19

    Applicant: 충남대학교산학협력단

    Inventor： 김철중 ,  이승훈 ,  홍석일 ,  부하령 ,  신광순 ,  이종수

    IPC: C12N15/86

    Abstract: PURPOSE: An Alphavirus expression vector capable of expressing a cancer-inhibiting protein, its producing method and its use are provided, thereby effectively inhibiting and preventing the growth of a cancer cell. CONSTITUTION: The Alphavirus expression vector capable of expressing a cancer-inhibiting protein contains a first cancer-inhibiting gene which is operably linked under operation of a first subgenomic promoter, a second cancer-inhibiting gene which is operably linked under operation of a second subgenomic promoter, and a third cancer-inhibiting gene which is operably linked under operation of a third subgenomic promoter, in which the cancer-inhibiting gene is selected from pRb/p110 or its homologue p107 and p120, p53 protein, p21 protein, p21 protein(WAF-1), bak, BRCA1, BRCA2, PTEN, APC, DCC, NF-1, NF-2, WT-1, MEN-I, MEN-II, zac1, p73, VHL, FCC and MCC; and the Alphavirus is Sindbis virus or Semliki Forest virus(SFV).

    Abstract translation: 目的：提供一种能够表达癌症抑制蛋白的甲病毒表达载体，其生产方法及其用途，由此有效地抑制和预防癌细胞的生长。 构成：能够表达癌症抑制蛋白的甲病毒表达载体含有第一种癌症抑制基因，该基因在第一亚基因组启动子的操作下可操作地连接，第二癌基因是在第二亚基因组启动子操作下可操作地连接的 和在第三亚基因组启动子的操作下可操作连接的第三癌抑制基因，其中所述癌抑制基因选自pRb / p110或其同源物p107和p120，p53蛋白，p21蛋白，p21蛋白（WAF -1），bak，BRCA1，BRCA2，PTEN，APC，DCC，NF-1，NF-2，WT-1，MEN-1，MEN-II，zac1，p73，VHL，FCC和MCC; 而α病毒是辛德毕斯病毒或塞姆利基森林病毒（SFV）。

    암억제 단백질을 발현하는 알파바이러스계 발현 벡터,이의 제조 방법 및 용도
    9.
    发明公开
    암억제 단백질을 발현하는 알파바이러스계 발현 벡터,이의 제조 방법 및 용도 有权
    基于ALPHAVIRUS的表达载体表达肿瘤抑制蛋白，其制备方法及其用途同时显示各种肿瘤抑制蛋白和有效抑制肿瘤的作用

    公开(公告)号：KR1020040077600A

    公开(公告)日：2004-09-04

    申请号：KR1020040060593

    申请日：2004-07-30

    Applicant: 충남대학교산학협력단

    Inventor： 김철중 ,  이승훈 ,  홍석일 ,  부하령 ,  신광순 ,  이종수

    IPC: A61K48/00 ,  C12N15/86

    CPC classification number: A61K48/00 ,  A61K2039/585 ,  C12N15/85

    Abstract: PURPOSE: Alphavirus-based expression vectors expressing tumor suppressor proteins, a method for preparing the same and the use thereof are provided, thereby simultaneously expressing various tumor suppressor proteins, and consequently suppressing tumor effectively. CONSTITUTION: The alphavirus-based expression vector expressing tumor suppressor proteins comprises a first tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a first subgenomic promoter; a second tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a second subgenomic promoter; and a third tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a third subgenomic promoter, wherein the first, second and third tumor suppressing genes or angiogenesis suppressing genes are the same or different; the tumor suppressing gene is p53 gene or PTEN gene; the antigiogenesis suppressing gene is angiostatin gene; and the alphavirus-based expression vector expresses tumor suppressor proteins Semliki forest virus(SFV) and Sindbis virus.

    Abstract translation: 目的：提供表达肿瘤抑制蛋白的基于甲病毒的表达载体，其制备方法及其用途，同时表达各种肿瘤抑制蛋白，从而有效抑制肿瘤。 构成：表达肿瘤抑制蛋白的基于甲病毒的表达载体包含与第一亚基因组启动子有效连接的第一肿瘤抑制基因或血管发生抑制基因; 与第二亚基因组启动子可操作地连接的第二肿瘤抑制基因或血管发生抑制基因; 和与第三亚基因组启动子可操作地连接的第三肿瘤抑制基因或血管发生抑制基因，其中第一，第二和第三肿瘤抑制基因或血管发生抑制基因相同或不同; 肿瘤抑制基因是p53基因或PTEN基因; 抗血管生成抑制基因是血管抑素基因; 并且基于甲病毒的表达载体表达肿瘤抑制蛋白Semliki森林病毒（SFV）和辛德毕斯病毒。

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