公开(公告)号：KR101701105B1

公开(公告)日：2017-02-01

申请号：KR1020150148539

申请日：2015-10-26

Applicant: 충남대학교산학협력단

Inventor： 이준헌 ,  서동원 ,  술타나 ,  최누리

IPC: C12Q1/68 ,  C12N9/90

Abstract: 본발명은한국토종오리의피모색을구분하기위한유전자마커및 이의용도에관한것으로, 본발명의한국토종오리의피모색을구분하기위하여개발된분자마커를통하여오리의피모색형질과연관된유전정보를제공함으로써오리의효율적인품종식별, 품종보호및 1대잡종의검정이가능하고, 피모색의변화로소비자의기호도를증진시킬수 있는품종개발이가능할뿐만아니라한국토종오리의피모색형질조절을통한종 개량에활용할수 있을것으로기대된다.

公开(公告)号：KR101474284B1

公开(公告)日：2014-12-24

申请号：KR1020120115246

申请日：2012-10-17

Applicant: 충남대학교산학협력단 ,  경상대학교산학협력단

Inventor： 이준헌 ,  임현태 ,  서동원 ,  최누리

IPC: C12N15/11 ,  C12Q1/68

Abstract: 본발명은마이크로새틀라이트마커를이용한재래닭의개체식별방법에관한것으로, 보다상세하게는마이크로새틀라이트마커인, LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, ADL0304로이루어진제1세트, LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, ADL0304, ADL0324, MCW0063, MCW0264로이루어진제2세트중 한세트이상을조합한마이크로새틀라이트마커를이용하여 PCR 증폭하는단계및 상기증폭단계에서증폭된산물을전기영동장치를이용해대립유전자를검출하고크기를분석하여유전자형을결정하는단계를포함하는재래닭의개체식별방법을제공한다. 본발명에따른방법은높은개체식별력을가지고있는마이크로새틀라이트마커를이용함으로재래닭의식별에형태학적인분류기준보다정확하게재래닭의개체식별과친자감별이가능하다. 따라서본 발명의방법을재래닭의개체식별에적용하여재래닭의보전및 신뢰도에긍정적인영향으로불법유통을제어하고, 재래닭산업의발전과농가의소득증대에기여할수 있다.

公开(公告)号：KR101997453B1

公开(公告)日：2019-07-09

申请号：KR1020180009264

申请日：2018-01-25

Applicant: 충남대학교산학협력단

Inventor： 이준헌 ,  조성현 ,  서동원 ,  최누리 ,  진실

IPC: C12Q1/6883

公开(公告)号：KR1020140049247A

公开(公告)日：2014-04-25

申请号：KR1020120115246

申请日：2012-10-17

Applicant: 충남대학교산학협력단 ,  경상대학교산학협력단

Inventor： 이준헌 ,  임현태 ,  서동원 ,  최누리

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q2525/151 ,  C12Q2600/158

Abstract: The present invention relates to a method for individually identifying Korean native chickens using a microsatellite marker, and more specifically, provides a method for individually identifying Korean native chickens, the method comprising: a step of performing PCR amplification using microsatellite markers obtained by combining one or more sets of a first set consisting of microsatellite markers LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, and ADL0304, and a second set consisting of microsatellite markers LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, ADL0304, ADL0324, MCW0063, and MCW0264; and determining the genotype by performing electrophoresis on the product amplified in the step of performing PCR amplification to thereby detect alleles and analyze sizes thereof. The method according to the present invention enables individual identification and paternity identification in a more accurate manner than the morphological classification, in terms of identification of Korean native chickens, by using the microsatellite markers having high individual identification. Therefore, the method of the present invention is applied to the individual identification of Korean native chickens to produce positive effects in the preservation of Korean native chickens and reliability thereof, thereby preventing illegal distribution and contributing to the development of native chicken industry and an increase in the farm income.

Abstract translation: 本发明涉及使用微卫星标记单独鉴定韩国本地鸡的方法，更具体地说，提供了一种用于单独鉴定韩国天然鸡的方法，所述方法包括：使用通过组合一个或多个的微卫星标记进行PCR扩增的步骤， 由微卫星标记LEI0251，ADL0259，ADL0021，MCW0104，MCW0261，MCW0087，ADL0292，GCT0016，MCW0029，MCW0145，LEI0107和ADL0304组成的第一组的更多组，以及由微卫星标记LEI0251，ADL0259，ADL0021，MCW0104组成的第二组 ，MCW0261，MCW0087，ADL0292，GCT0016，MCW0029，MCW0145，LEI0107，ADL0304，ADL0324，MCW0063和MCW0264; 以及通过对进行PCR扩增的步骤中扩增的产物进行电泳从而检测等位基因并分析其大小来确定基因型。 根据本发明的方法，通过使用具有高个体鉴定的微卫星标记，在韩国本地鸡的鉴定方面，以比形态分类更准确的方式进行个体鉴定和亲戚鉴定。 因此，本发明的方法适用于韩国本土鸡的个体鉴定，从而在保护韩国本国鸡中产生积极作用及其可靠性，从而防止非法分配和促进本地鸡业发展，并增加 农场收入。