公开(公告)号：KR1020110022759A

公开(公告)日：2011-03-08

申请号：KR1020090080159

申请日：2009-08-28

Applicant: 충남대학교산학협력단

Inventor： 김민규 ,  최선아 ,  이지혜 ,  김근중

IPC: C12N5/07 ,  C12N5/02

Abstract: PURPOSE: A cell therapeutic agent containing amniotic fluid-derived mesenchymal stem cells is provided to ensure superior differentiation ability. CONSTITUTION: A method for preparing canine amniotic fluid-derived mesenchymal stem cells comprises: a step of collecting amniotic fluid from a pregnant dog using a syringe; a step of centrifuging amniotic fluid to collect pellet; a step of culturing the pellet in a medium containing bFGF(basic fibroblast growth factor) and EGF(epidermal growth factor) and collecting cultured cells; and a step of isolating and purifying the stem cells. The method for further comprises a step of inducing cell adhesion. The medium contains 0.5-20 ng of bFGF, 1-40 ng of EGF, 1-40% FBS(fetal bovine serum), and 0.5-4% penicillin-streptomycin. A composition for controlling differentiation of mesenchymal stem cells to adipocytes contains FBS, IBMX, dexamethasone, indomethacin, and insulin as active ingredient.

Abstract translation: 目的：提供含有羊水间充质干细胞的细胞治疗剂，以确保优异的分化能力。 构成：用于制备犬羊水间充质干细胞的方法包括：使用注射器从怀孕犬采集羊水的步骤; 离心羊水收集颗粒的步骤; 在含有bFGF（碱性成纤维细胞生长因子）和EGF（表皮生长因子）的培养基中培养该颗粒并收集培养的细胞的步骤; 以及分离和纯化干细胞的步骤。 该方法还包括诱导细胞粘附的步骤。 培养基含有0.5-20ng的bFGF，1-40ng的EGF，1-40％的FBS（胎牛血清）和0.5-4％的青霉素 - 链霉素。 用于控制间充质干细胞分化为脂肪细胞的组合物含有FBS，IBMX，地塞米松，吲哚美辛和胰岛素作为活性成分。