公开(公告)号：KR1020110119032A

公开(公告)日：2011-11-02

申请号：KR1020100038498

申请日：2010-04-26

Applicant: 충남대학교산학협력단

Inventor： 김홍기 ,  최진수 ,  송정영 ,  임용표

IPC: C12Q1/24 ,  C12M1/12 ,  C12N1/00

CPC classification number: C12Q1/24 ,  C12N1/14 ,  C12N2509/10

Abstract: PURPOSE: A method for isolating hypnospores of Plasmodiophora brassicae and a method for proliferating using the same are provided to enable molecular biological study. CONSTITUTION: A method for isolating hypnospores from infected plants or soil comprises: a step of pretreating infected plants or soil to washed Plasmodiophora brassicae bacteria; a step of removing impurities from the plants or soil using a fabric made of cotton, silk, rayon, or synthetic fiber; a step of isolating prepared suspension with a cellulose filter; and a step of centrifuging the isolated suspension and centrifuging to prepare a pellet. The Plasmodiophora brassicae bacteria is Plasmodiophora brassicae.

Abstract translation: 目的：分离生物学研究提供了一种分离豌豆瘟菌孢子的方法和使用该方法进行增殖的方法。 构成：从受感染的植物或土壤中分离催乳素的方法包括：对感染的植物或土壤进行预处理以洗涤堇青霉菌的步骤; 使用由棉，丝，人造纤维或合成纤维制成的织物从植物或土壤中除去杂质的步骤; 用纤维素过滤器分离制备的悬浮液的步骤; 以及离心分离的悬浮液并离心以制备颗粒的步骤。 疟原虫细菌是Plasmodiophora brassicae。

公开(公告)号：KR1020110119038A

公开(公告)日：2011-11-02

申请号：KR1020100038505

申请日：2010-04-26

Applicant: 충남대학교산학협력단

Inventor： 김홍기 ,  최진수 ,  송정영 ,  임용표

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/686 ,  C12Q1/6895 ,  C12Q2600/13

Abstract: PURPOSE: A primer which specifically binds to clubroot and a method for diagnosing clubroot of Brassicaceae crops using the same are provided. CONSTITUTION: A method for diagnosing clubroot of Brassicaceae crops comprises: a step of performing PCR of DNA isolated from a spore of Brassicaceae crops from crops and soil under the presence of a primer set which specifically amplifies ITS(interual transcribed spacer) gene; and a step of analyzing gene amplification to determine club root of Brassicaceae crops. The primer set contains a forward primer of sequence number 1, 3, 5, or 7 and a reverse primer of sequence number 2, 4, 6, or 8.

Abstract translation: 目的：提供一种特异性结合棒状根的引物，并提供了一种用于诊断使用其的芥菜科作物的根茎的方法。 构成：用于诊断芸苔属植物的根茎的方法包括：在特异性扩增ITS（相互转录的间隔基）基因的引物组的存在下，从作物和土壤中进行从芥菜科作物的孢子分离的DNA的PCR步骤; 以及分析基因扩增以确定甘蓝科植物作物的根系的步骤。 引物组包含序列号1,3,5或7的正向引物和序列号为2,4,6或8的反向引物。