公开(公告)号：KR1020100047940A

公开(公告)日：2010-05-11

申请号：KR1020080106874

申请日：2008-10-30

Applicant: 충남대학교산학협력단

Inventor： 서상희 ,  김희만 ,  황선도 ,  김윤희 ,  신진수 ,  구근본

IPC: C12N7/01 ,  C12N7/00 ,  C12N15/33 ,  C12N15/09

Abstract: PURPOSE: A vaccine for preventing H5N1 serotype highly pathogenic avian influenza is provided to prevent recurrence of H5type and disease. CONSTITUTION: A method for manufacturing H5N1 serotype highly pathogenic avian influenza virus strain(KCTC 11405BP) comprises: a step of amplifying a highly pathogenic gene from A/Vietnam/1203/2004 (H5N1) strain; a step of transforming by recombination of the gene; a third step of amplifying the gene from A/PR/8/34 (H1N1); a step of culturing plasmid in Vero cells; and a step of inoculating culture supernatant to specific pathogen free(SPF) a hatching egg. The H5N1 serotype highly pathogenic avian influenza virus strain(KCTC 11405BP) contains the highly pathogen-inducing gene. The gene contains HA, NA, PB2, PB1, PA, NP, M and NS.

Abstract translation: 目的：提供一种预防H5N1血清型高致病性禽流感疫苗，以预防H5型和疾病的复发。 构成：制备H5N1血清型高致病性禽流感病毒株（KCTC 11405BP）的方法包括：从A / Vietnam / 1203/2004（H5N1）菌株扩增高致病基因的步骤; 通过基因重组转化的步骤; 扩增A / PR / 8/34（H1N1）基因的第三步; 在Vero细胞中培养质粒的步骤; 以及将培养上清液接种到特定无病原体（SPF）孵化蛋的步骤。 H5N1血清型高致病性禽流感病毒株（KCTC 11405BP）含有高致病性诱导基因。 该基因含有HA，NA，PB2，PB1，PA，NP，M和NS。