公开(公告)号：KR101151239B1

公开(公告)日：2012-06-14

申请号：KR1020090035772

申请日：2009-04-24

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  훵휘

IPC: C12N15/29 ,  C12Q1/68 ,  C12N15/11

Abstract: PURPOSE: A molecular marker of genetic multiple allele male sterile gene of brassica campestris ssp. Pekinensis and genetic map of brassica campestris ssp. Pekinensis are provided to ensure effectiveness in map-based cloning. CONSTITUTION: A genetic map of brassica campestris ssp. Pekinensis comprises a genetic multiple-allele male sterile gene Ms and SSR marker to the same. The gene Ms is located in chromosome R07. The SSR marker is cnu_m273, cnu_m030, cnu_m295, or syau_m13. A primer pair to cnu_m273 has sequence numbers 3 and 4. A primer pair to cnu_m030 has sequence numbers 5 and 6. A primer pair to cnu_m295 has sequence numbers 7 and 8. A method for identifying gene Ms comprises: a step of isolating genome DNA from brassica campestris ssp. Pekinensis; a step of amplifying the genome DNA; and a step of detecting amplified product.

公开(公告)号：KR1020100117170A

公开(公告)日：2010-11-03

申请号：KR1020090035772

申请日：2009-04-24

Applicant: 충남대학교산학협력단

Inventor： 임용표 ,  훵휘

IPC: C12N15/29 ,  C12Q1/68 ,  C12N15/11

CPC classification number: C12N15/11 ,  C12Q1/6844 ,  C12Q1/6858 ,  C12Q2600/156

Abstract: PURPOSE: A molecular marker of genetic multiple allele male sterile gene of brassica campestris ssp. Pekinensis and genetic map of brassica campestris ssp. Pekinensis are provided to ensure effectiveness in map-based cloning. CONSTITUTION: A genetic map of brassica campestris ssp. Pekinensis comprises a genetic multiple-allele male sterile gene Ms and SSR marker to the same. The gene Ms is located in chromosome R07. The SSR marker is cnu_m273, cnu_m030, cnu_m295, or syau_m13. A primer pair to cnu_m273 has sequence numbers 3 and 4. A primer pair to cnu_m030 has sequence numbers 5 and 6. A primer pair to cnu_m295 has sequence numbers 7 and 8. A method for identifying gene Ms comprises: a step of isolating genome DNA from brassica campestris ssp. Pekinensis; a step of amplifying the genome DNA; and a step of detecting amplified product.

Abstract translation: 目的：芸苔属植物遗传多重等位基因雄性不育基因的分子标记。 北京菜和芸苔属植物的遗传图谱。 提供Pekinensis以确保基于地图的克隆的有效性。 构成：芸苔属植物的遗传图谱。 北美包含遗传多重等位基因雄性不育基因Ms和SSR标记。 基因Ms位于染色体R07中。 SSR标记为cnu_m273，cnu_m030，cnu_m295或syau_m13。 cnu_m273的引物对具有序列号3和4.与cnu_m030的引物对具有序列号5和6。cnu_m295的引物对具有序列号7和8.鉴定基因Ms的方法包括：分离基因组DNA的步骤 从芸苔菜 大白菜; 扩增基因组DNA的步骤; 以及检测扩增产物的步骤。