公开(公告)号：KR101962373B1

公开(公告)日：2019-03-26

申请号：KR1020170006198

申请日：2017-01-13

Applicant: 포항공과대학교 산학협력단 ,  가톨릭대학교 산학협력단

Inventor： 김현지 ,  고현선 ,  장진아 ,  신종철 ,  조동우 ,  하동헌

IPC: A61B17/42 ,  A61B17/00

公开(公告)号：KR1020110087674A

公开(公告)日：2011-08-03

申请号：KR1020100007205

申请日：2010-01-27

Applicant: 가톨릭대학교 산학협력단

Inventor： 조영애 ,  오은주 ,  안현영 ,  신종철

IPC: C12N5/071 ,  C12N5/078 ,  C12N5/02

Abstract: PURPOSE: An ex vivo method for proliferating endothelial progenitor cells(EPCs) is provided to effectively proliferate EPCs and prevent or treat anginogenesis and ischemic diseases. CONSTITUTION: A method for proliferating EPCs comprises a step of culturing CD34+ cel fraction in a serum-free medium containing SCF(stem cell factor), FLT-3L(FLT-3 ligand), IL-3, and bFGF(basic fibroblast growth factor). The culture is a non-adhesive ex vivo culture. The EPCs are derived from bone marrow, peripheral blood, or umbilical cord blood.

Abstract translation: 目的：提供增强内皮祖细胞（EPCs）的离体方法，有效增殖EPCs，预防或治疗血管发生和缺血性疾病。 构成：增殖EPCs的方法包括在含有SCF（干细胞因子），FLT-3L（FLT-3配体），IL-3和bFGF（碱性成纤维细胞生长因子）的无血清培养基中培养CD34 +细胞级分的步骤 ）。 培养物是一种非粘性离体培养物。 EPCs来源于骨髓，外周血或脐带血。

公开(公告)号：KR101132858B1

公开(公告)日：2012-04-03

申请号：KR1020100007205

申请日：2010-01-27

Applicant: 가톨릭대학교 산학협력단

Inventor： 조영애 ,  오은주 ,  안현영 ,  신종철

IPC: C12N5/071 ,  C12N5/078 ,  C12N5/02

Abstract: PURPOSE: An ex vivo method for proliferating endothelial progenitor cells(EPCs) is provided to effectively proliferate EPCs and prevent or treat anginogenesis and ischemic diseases. CONSTITUTION: A method for proliferating EPCs comprises a step of culturing CD34+ cel fraction in a serum-free medium containing SCF(stem cell factor), FLT-3L(FLT-3 ligand), IL-3, and bFGF(basic fibroblast growth factor). The culture is a non-adhesive ex vivo culture. The EPCs are derived from bone marrow, peripheral blood, or umbilical cord blood.