公开(公告)号：KR100410784B1

公开(公告)日：2003-12-31

申请号：KR1020010016517

申请日：2001-03-29

Applicant: 학교법인연세대학교

Inventor： 최강열 ,  김선홍 ,  권형배 ,  김경섭 ,  안용호

IPC: C12N15/55

Abstract: PURPOSE: A dual specific phosphatase with anticancer activity is provided. It specifically inhibits ERK(extra-cellular regulated kinase) activity and proceeding of from G1 to S, therefore, it is useful for the treatment of cancer associated with MAP-kinase(mitogen-activated protein kinase) pathway. CONSTITUTION: The drosophila MAPK phosphatase gene specifically inhibiting the ERK has the nucleotide sequence of SEQ ID NO: 1, in which the ERK is derived from mammals or Drosophila. An expression vector containing the drosophila MAPK phosphatase gene is selected from the group consisting of pOT2-DMKP-3, pMT/V5-DMKP-3, pPacPL-DMKP-3, pcDNA3.1-DMKP-3 and pB42AD-DMKP-3. The drosophila MAPK phosphatase has the amino acid sequence of SEQ ID NO: 2.

Abstract translation: 目的：提供具有抗癌活性的双特异性磷酸酶。 它特异性地抑制ERK（细胞外调节激酶）活性和从G1到S的进程，因此它可用于治疗与MAP-激酶（促分裂原活化蛋白激酶）途径相关的癌症。 构成：特异性抑制ERK的果蝇MAPK磷酸酶基因具有SEQ ID NO：1的核苷酸序列，其中ERK来源于哺乳动物或果蝇。 含有果蝇MAPK磷酸酶基因的表达载体选自pOT2-DMKP-3，pMT / V5-DMKP-3，pPacPL-DMKP-3，pcDNA3.1-DMKP-3和pB42AD-DMKP-3。 果蝇MAPK磷酸酶具有SEQ ID NO：2的氨基酸序列。

公开(公告)号：KR1020020076562A

公开(公告)日：2002-10-11

申请号：KR1020010016517

申请日：2001-03-29

Applicant: 학교법인연세대학교

Inventor： 최강열 ,  김선홍 ,  권형배 ,  김경섭 ,  안용호

IPC: C12N15/55

CPC classification number: C12N9/16 ,  C12Y301/03048

Abstract: PURPOSE: A dual specific phosphatase with anticancer activity is provided. It specifically inhibits ERK(extra-cellular regulated kinase) activity and proceeding of from G1 to S, therefore, it is useful for the treatment of cancer associated with MAP-kinase(mitogen-activated protein kinase) pathway. CONSTITUTION: The drosophila MAPK phosphatase gene specifically inhibiting the ERK has the nucleotide sequence of SEQ ID NO: 1, in which the ERK is derived from mammals or Drosophila. An expression vector containing the drosophila MAPK phosphatase gene is selected from the group consisting of pOT2-DMKP-3, pMT/V5-DMKP-3, pPacPL-DMKP-3, pcDNA3.1-DMKP-3 and pB42AD-DMKP-3. The drosophila MAPK phosphatase has the amino acid sequence of SEQ ID NO: 2.

Abstract translation: 目的：提供具有抗癌活性的双重特异性磷酸酶。 它特异性地抑制ERK（细胞外调节激酶）活性和从G1到S的进行，因此，它可用于治疗与MAP-激酶（丝裂原活化蛋白激酶）途径相关的癌症。 构成：特异性抑制ERK的果蝇MAPK磷酸酶基因具有SEQ ID NO：1的核苷酸序列，其中ERK衍生自哺乳动物或果蝇。 含有果蝇MAPK磷酸酶基因的表达载体选自pOT2-DMKP-3，pMT / V5-DMKP-3，pPacPL-DMKP-3，pcDNA3.1-DMKP-3和pB42AD-DMKP-3。 果蝇MAPK磷酸酶具有SEQ ID NO：2的氨基酸序列。