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公开(公告)号:KR100262769B1
公开(公告)日:2000-08-01
申请号:KR1019970074490
申请日:1997-12-26
Applicant: 한국과학기술연구원
IPC: C07K1/14
Abstract: PURPOSE: Provided is an aqueous and hetero phase separation segregate system consisting of a high molecular aqueous solution of polyethylene glycol and levan, to separate proteins in large quantity with low cost. And a protein separation system using the same is also provided. CONSTITUTION: An aqueous and hetero phase separation segregate system consisting of a high molecular aqueous solution of polyethylene glycol and levan is composed of the following steps of: (a) preparing polyethylene glycol-levan hetero phase segregation system; (b) searching phase diagram of polyethylene glycol-levan; and (c) searching distribution coefficient according to the change of concentration of salt and pH value.
Abstract translation: 目的:提供由高分子量聚乙二醇水溶液和levan组成的水相和异相分离分离系统,以低成本大量分离蛋白质。 还提供了使用其的蛋白质分离系统。 构成:由高分子量聚乙二醇水溶液和levan组成的水相和异相分离分离系统由以下步骤组成:(a)制备聚乙二醇 - 异戊二烯异相分离系统; (b)搜索聚乙二醇 - 叶子的相图; 和(c)根据盐浓度和pH值的变化搜索分布系数。